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J Chromatogr B Biomed Sci Appl ; 697(1-2): 263-8, 1997 Sep 12.
Article in English | MEDLINE | ID: mdl-9342679

ABSTRACT

Capillary zone electrophoresis with UV absorbance detection was used to separate tryptophan and ten of its metabolites. Run buffers of pH 4.0-10.0 were evaluated for their effect on resolution; a pH 9.6 buffer was found to give optimum separation of all components. Ethylenediaminetetraacetic acid (EDTA), which prevents complexation of some analytes with polyvalent cations, was included in the run buffer to insure good peak shape and reproducible mobilities. The resulting method was used to detect the presence of quinolinic acid in a urine sample.


Subject(s)
Quinolinic Acid/urine , Tryptophan/isolation & purification , Buffers , Edetic Acid , Electrophoresis, Capillary , Humans , Hydrogen-Ion Concentration , Reproducibility of Results , Spectrophotometry, Ultraviolet , Tryptophan/metabolism
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