1.
Bioconjug Chem
; 4(3): 212-8, 1993.
Article
in English
| MEDLINE
| ID: mdl-8324011
ABSTRACT
p-Maleimidophenyl isocyanate (PMPI, 1) is a heterobifunctional cross-linking agent useful for thiol to hydroxyl coupling. Several maleimide-activated compounds were prepared and characterized and then shown to be reactive with thiol-containing proteins. Examples include activation of vitamin B12, digoxigenin, digitoxigenin, estradiol, progesterone, and some serine-containing peptides.
Subject(s)
Cross-Linking Reagents/chemistry , Cyanates/chemistry , Maleimides/chemistry , Sulfhydryl Compounds/chemistry , Alkaline Phosphatase/chemistry , Amino Acid Sequence , Cross-Linking Reagents/chemical synthesis , Cyanates/chemical synthesis , Digoxigenin/chemistry , Drug Stability , Estradiol/chemistry , Haptens/chemistry , Maleimides/chemical synthesis , Molecular Sequence Data , Peptides/chemistry , Vitamin B 12/chemistry
2.
