ABSTRACT
The expression of S100B and S100A6 mRNA in CA1 region of rat hippocampal sections was studied after tetanizing stimulation. The level of S100B expression increased 2-4-fold in comparison with the control after 30 min and gradually returned to the basal level 120 min after tetanization. The level of S100A6 mRNA was very low and did not change after tetanization.
Subject(s)
Cell Cycle Proteins/genetics , Gene Expression Regulation , Hippocampus/metabolism , Long-Term Potentiation/genetics , Nerve Growth Factors/genetics , S100 Proteins/genetics , Animals , Male , Polymerase Chain Reaction , Rats , Rats, Wistar , S100 Calcium Binding Protein A6 , S100 Calcium Binding Protein beta SubunitABSTRACT
We studied the effect of potentiated antibodies to morphine (10(-100) wt %) on self-stimulation of the lateral hypothalamus and behavioral reactions reflecting the severity of withdrawal syndrome in rats with morphine dependence. Repeated treatment with potentiated antibodies to morphine increased the rate of self-stimulation, suppressed active avoidance response, promoted freezing behavior after acoustic stimulation, and decreased tail-flick latency in rats after morphine withdrawal. Distilled water did not produce these changes.
Subject(s)
Antibodies/pharmacology , Behavior, Animal/drug effects , Morphine Dependence , Morphine/antagonists & inhibitors , Animals , Brain/drug effects , Brain/metabolism , Male , Morphine/immunology , Rats , Self Stimulation/drug effectsABSTRACT
Antibodies against S100 protein in ultralow doses specifically affected catecholamine metabolism in rats withdrawn from chronic ethanol exposure. The contents of tryptophan, tyrosine, and norepinephrine in brain structures returned to normal. The concentrations of dopamine, epinephrine, and norepinephrine in the peripheral blood decreased. Modulation of monoamine content in the peripheral blood suggests that antibodies against S100 protein possess stress-protective activity during ethanol withdrawal.
Subject(s)
Antibodies/pharmacology , Biogenic Amines/metabolism , Brain/drug effects , Brain/metabolism , Ethanol/toxicity , Lipid Peroxidation/drug effects , S100 Proteins/immunology , Animals , Dopamine/metabolism , Epinephrine/metabolism , Male , Norepinephrine/blood , Norepinephrine/metabolism , Rats , Substance Withdrawal Syndrome/drug therapy , Tryptophan/blood , Tyrosine/bloodABSTRACT
We compared the effects of neurotropic and neurospecific substances and their antibodies on conditioned activity of rats. Single treatment produced the positive effect on the latency and number of conditioned responses. Repeated treatment with test compounds in the same dose improved conditioned activity of animals.
Subject(s)
Antibodies/pharmacology , Behavior, Animal/drug effects , Ethanol/pharmacology , Morphine/pharmacology , S100 Proteins/pharmacology , Animals , Antibodies/immunology , Male , Morphine/immunology , Rats , Receptors, Opioid, mu/immunology , S100 Proteins/immunologyABSTRACT
We studied the effects of ethanol, morphine, S100 protein, and antibodies to morphine, S100 protein, and opiate -receptors in ultralow doses on self-stimulation of the lateral hypothalamus. The reaction underwent similar changes after single administration of test preparations. Tenfold treatment produced the stimulatory and stabilizing effect, which was related to ambivalent properties of preparations in ultralow doses. Tenfold administration of water did not produce changes in control animals.
Subject(s)
Antibodies/pharmacology , Brain/drug effects , Brain/metabolism , Ethanol/pharmacology , Morphine/pharmacology , Animals , Antibodies/immunology , Behavior, Animal/drug effects , Hypothalamic Area, Lateral/drug effects , Hypothalamic Area, Lateral/metabolism , Male , Morphine/immunology , Rats , Receptors, Opioid, mu/immunology , S100 Proteins/immunology , S100 Proteins/pharmacology , Self Stimulation/drug effectsABSTRACT
The efficiency of potentiated antibodies against morphine was studied on the model of chronic morphine intoxication. Test antibodies stimulated catecholamine metabolism in the hypothalamus (i.e., prevented initiation of catecholamine- and histaminergic peripheral reactions) and normalized lipid peroxidation.
