Impact of Grazing on Diversity of Semi-Arid Rangelands in Crete Island in the Context of Climatic Change.

The rangelands of Crete island (Greece) are typical Mediterranean habitats under high risk of degradation due to long-term grazing and harsh climatic conditions. We explored the effect of abiotic (climatic conditions, altitude) and biotic factors (long-term grazing by small ruminants) on the floristic composition and diversity of selected lowland (Pyrathi, Faistos) and highland (Vroulidia, Nida) rangelands. In each rangeland, the ground cover was measured, and the floristic composition was calculated in terms of five functional groups: grasses, legumes, forbs, phrygana, and shrubs. The aridity index, species turnover, species richness, Shannon entropy, and Gini-Simpson index (with the latter two converted to the effective number of species) were calculated. Our results reveal that highlands are characterized by the highest aridity index (wetter conditions). Lowland rangelands, compared to highland, exhibited a higher percentage contribution of grasses, legumes, and forbs, while species turnover decreased along the altitudinal gradient. The Shannon entropy index was correlated (a) positively with Gini-Simpson and mean annual temperature and (b) negatively with mean annual precipitation, aridity index, and altitude. Moreover, the Gini-Simpson index correlated positively with mean annual temperature and negatively with altitude. Our results could help to understand the effects of grazing on rangeland dynamics and sustainability in semi-arid regions in the context of climatic change.

Toxicity effects of olive-mill wastewater on growth, photosynthesis and pollen morphology of spinach plants.

Olive mill-wastewater (OMW), a by-product of the olive oil extraction process, represents a significant environmental problem in Mediterranean areas. We studied the impact of OMW dilutions (1:10 and 1:20) on growth, photosynthesis, proline and sugar accumulation as well as on pollen morphology of spinach (Spinacia oleracea L.) plants, to evaluate the application of OMW dilutions as pretreatment technique, prior to land disposal. Biomass, height, total chlorophyll and leaf area of spinach declined progressively with decreasing OMW dilution. Since fatty acids and phenolic compounds (present in the OMW) are considered precursors in the polymerization of sporopollenin, we suggest that under OMW treatment spinach plants seem to 'direct' the excess of these substances in the production and formation of increased pollen grains. Proline did not accumulate under OMW stress, but decreased possible due to transport to pollens in response to increased demand to over-production of pollens. Both OMW dilutions resulted in a decreased efficiency of PSII functioning and an increased excitation pressure (1-q(p)). It is concluded that, higher than 1:20 OMW dilutions should be used, and/or additional treatment should be applied before use of the OMW in the environment.