ABSTRACT
We have studied the influence of dietary ω-3 polyunsaturated fatty acids (ω-3 PUFA) on the functioning of subsarcolemmal and interfibrillar mitochondrial fractions of rat myocardium, changes in expression of cytochrome P450 (CYP2E1) and the activity of enzymes of prooxidant-antioxidant system after isoproterenol-induced myocardial injury. It has been found that in vivo administration of ω-3 PUFA (Epadol 0.1 ml/100 gr of weight for 4 weeks) significantly reduced the swelling of subsarcolemmal and interfibrillar mitochondrial fractions by 65.52% 54.84% respectively, pointing for a decrease of damage of the mitochondrial function evoked by in vivo administration of isoproterenol. In vivo administration of ω-3 PUFAs prevents a decrease in the activity of antioxidant enzymes catalase and superoxide dismutase (2.65 and 7.1- fold, respectively) after isoproterenol-induced myocardial injury. We suggest that the development of oxidative stress after isoproterenol-induced myocardial injury can be caused by a significant increase in the expression of cytochrome P450 2E1 (73.3%), and administration of ω-3 PUFAs prevents such changes.
Subject(s)
Cardiomyopathies/diet therapy , Cardiotonic Agents/pharmacology , Cytochrome P-450 CYP2E1 Inhibitors/pharmacology , Cytochrome P-450 CYP2E1/genetics , Fatty Acids, Omega-3/pharmacology , Mitochondria, Heart/drug effects , Administration, Oral , Animals , Cardiomyopathies/chemically induced , Cardiomyopathies/metabolism , Cardiomyopathies/pathology , Catalase/genetics , Catalase/metabolism , Cytochrome P-450 CYP2E1/metabolism , Gene Expression Regulation , Isoproterenol/administration & dosage , Isoproterenol/antagonists & inhibitors , Male , Mitochondria, Heart/metabolism , Myocardium/metabolism , Myocardium/pathology , Myocytes, Cardiac/drug effects , Myocytes, Cardiac/metabolism , Myocytes, Cardiac/pathology , Oxidative Stress , Rats , Rats, Wistar , Sarcolemma/drug effects , Sarcolemma/metabolism , Superoxide Dismutase/genetics , Superoxide Dismutase/metabolismABSTRACT
Patients with permanent atrial fibrillation in comparison with cardiovascular patients without arrhythmia were found to have higher level of serum uric acid irrespectively of background of arterial hypertension and/or ischemic heart disease. Serum uric acid was shown to be an independent predictor of chronic heart failure exacerbation in atrial fibrillation patients.
ABSTRACT
We have studied the functional parameters of mitochondria from hearts after isopreterenol-induced injury (two subcutaneous injections of isopreterenol at the dose 60 mg/kg/day). We investigated the influence of dietary omega-3 polyunsaturated fatty acids (omega-3 PUFAs) administered to rats (Epadol, 0.1 mg/100 gr of weight for 4 weeks) on these parameters. Isoproterenol-induced heart injury leads to a decreased parameters of respiration of isolated mitochondria in the presence of succinate. Administration of omega-3 PUFAs significantly restored the respiration rate of mitochondria: the state 3 respiration was increased by 70.12%, the state 4 by 39.87% and the respiratory control ratio by 45.19% compared to the corresponding parameters of experimental group. Also, it was shown the ability of omega-3 PUFAs to decrease mitochondria swelling (by 60%) in nominally free calcium solution. The results of the study indicate that omega-3 PUFAs improve the altered functions of the heart mitochondria evoked by isoproterenol-induced injury.
Subject(s)
Fatty Acids, Omega-3/therapeutic use , Mitochondria, Heart/drug effects , Mitochondrial Swelling/drug effects , Myocardial Infarction/prevention & control , Myocardium/pathology , Administration, Oral , Animals , Calcium/pharmacology , Disease Models, Animal , Fatty Acids, Omega-3/administration & dosage , Fatty Acids, Omega-3/pharmacology , Isoproterenol/pharmacology , Male , Mitochondria, Heart/metabolism , Mitochondria, Heart/pathology , Myocardial Infarction/chemically induced , Myocardial Infarction/metabolism , Myocardial Infarction/pathology , Myocardium/metabolism , Oxygen Consumption/drug effects , Phosphorylation , Rats , Succinic Acid/metabolismABSTRACT
Flurenizide, a new home drug preparation, endowed with antituberculosis activity, was administered to 20 patients with freshly detected pulmonary tuberculosis, in combination with etambutole or rifampycin in a daily dose of 0.6 g. After the above chemotherapy, closure of the cavities, diminution in size and consolidation of the foci as well as infiltrates, dispelling of the symptoms of intoxication, and discontinuance of bacteria discharge were observable in 70.0 percent of the cases. The drug is well tolerated by patients and is not associated with side effects. The results obtained permit recommending flurenizide for use in phthisiopulmonological practice.
Subject(s)
Antitubercular Agents/therapeutic use , Heterocyclic Compounds/therapeutic use , Tuberculosis, Pulmonary/drug therapy , Adolescent , Adult , Capsules , Drug Therapy, Combination , Ethambutol/therapeutic use , Female , Humans , Male , Middle Aged , Rifampin/therapeutic use , Time FactorsABSTRACT
Biopsy of the gastric and duodenal mucosa was carried out during diagnostic esophagogastroduodenofibroscopy. Histological examination was realized using the light polarization microscope. Amyloid was found in 48.8% with pathological changes in the urine. In patients without pathological changes of the urine amyloidosis was diagnosed bioptically in 8.6% of duodenal and 10.3% of gastric biopsies.
Subject(s)
Amyloidosis/pathology , Duodenum/pathology , Gastric Mucosa/pathology , Intestinal Mucosa/pathology , Lung Diseases, Obstructive/pathology , Tuberculosis, Pulmonary/pathology , Adult , Aged , Biopsy, Needle , Endoscopy, Digestive System , Female , Fiber Optic Technology , Humans , Male , Middle AgedABSTRACT
The authors developed a table differential-diagnostic algorithm designed for improvement of the differential diagnosis of pulmonary tuberculosis and cancer. This algorithm allows to establish precise diagnosis of cancer or tuberculosis of the lungs at district and regional health service levels, enhances the time of diagnosis and favours rational treatment.
Subject(s)
Algorithms , Lung Neoplasms/diagnosis , Tuberculosis, Pulmonary/diagnosis , Adolescent , Adult , Aged , Aged, 80 and over , Diagnosis, Differential , Humans , Male , Mathematics , Middle AgedABSTRACT
The allelopathic interaction between sorghum [Sorghum bicolor (L.) Moench] and 10 species of grass and broadleaf weeds was investigated. Germination of weed seeds was slightly inhibited or stimulated, depending on species, when incubated in closed Petri dishes with germinating sorghum. Subsequent radicle and hypocotyl or coleoptile elongation of weeds was significantly inhibited by the germinating sorghum. For weeds interplanted with sorghum and grown under greenhouse conditions. The inhibitory effect on some weed species was still evident after 2 months of growth. Significant differences were found in the dry matter per weed plant grown in pots in proximity to sorghum vs. weeds grown in monoculture. Aqueous leachates from pots planted with sorghum alone or from a system in which sorghum roots protruded into water had strong allelopathic activity. These results indicate that water-soluble allelochemicals are produced by germinating sorghum seeds and that production of these substances continues during seedling growth.
ABSTRACT
The responses of Colorado potato beetle,Leptinotarsa decemlineata (Say), to volatile components of tansy,Tanacetum vulgare L., were investigated in order to establish a chemical basis for observed reduction in beetle populations when potatoes,Solanum tuberosum L., were interplanted with tansy. Colorado potato beetles exhibited avoidance behavior to tansy oil, volatiles from intact tansy plants, a "hydrocarbon fraction" of tansy oil, obtained by fractionation on alumina, and five of the 13 known components of tansy oil that were tested. One constituent of tansy oil, α-pinene, attracted beetles.
