Subject(s)
Synovial Fluid/cytology , Synovitis/diagnosis , Adult , Cell Count , Diagnosis, Differential , Female , Humans , Knee Joint , Male , Middle Aged , Rheumatic Diseases/complications , Rheumatic Diseases/diagnosis , Synovial Fluid/chemistry , Synovial Fluid/immunology , Synovial Fluid/metabolism , Synovitis/etiologyABSTRACT
Synovial fluid (SF) contained by the knee joint from 102 patients (80 with rheumatoid arthritis, 12 with osteoarthrosis, 10 with posttraumatic arthritis) and controls was studied for cytosis, activity of acid phosphatase, N-acetyl-beta-D-glucosaminidase, and cAMP and cGMP content. In 18% of the cases, the local corticosteroid treatment of patients with RA produced no beneficial effect. To treat RA and OA patients, dimethylsulfoxide (DMSO, 5 ml of 20% solution) and polyvinylpyrrolidone (PVP, 5 ml of 15% solution) were administered intra-articularly. In OA patients, injection of DMSO and PVP provided a good therapeutic effect. In RA patients, injection of DMSO produced a beneficial antiinflammatory action but it appeared short-term. The use of PVP in RA patients with pronounced local process enhanced synovitis symptoms, entailed an increase of the SF amount and cytosis, did not reduce the activity of lysosomal enzymes, and produced a negligible effect on the level of cyclic nucleotides. A mixture of DMSO (1 ml of 100% solution) and PVP (5 ml of 15% solution) applied to the treatment of RA patients exerted a favourable action on the local process in the joint, namely reduced all the parameters mirroring the intensity of inflammation.
Subject(s)
Adrenal Cortex Hormones/administration & dosage , Arthritis, Rheumatoid/drug therapy , Dimethyl Sulfoxide/administration & dosage , Povidone/administration & dosage , Synovial Fluid/drug effects , Adult , Arthritis, Rheumatoid/metabolism , Chronic Disease , Drug Evaluation , Female , Humans , Injections, Intra-Articular , Male , Middle Aged , Osteoarthritis/drug therapy , Osteoarthritis/metabolism , Synovial Fluid/analysis , Synovitis/drug therapy , Synovitis/metabolismSubject(s)
B-Lymphocytes/metabolism , Palatine Tonsil/metabolism , Receptors, Immunologic/metabolism , T-Lymphocytes/metabolism , Tonsillitis/metabolism , Adult , Aging , B-Lymphocytes/immunology , Chronic Disease , Humans , Palatine Tonsil/immunology , Receptors, Immunologic/immunology , Rosette Formation , T-Lymphocytes/immunology , Tonsillitis/immunologyABSTRACT
The adsorption of human, sheep and human red blood cells and human lymphocytes on the colonies of streptococci in the L-form has been studied. Th electron-microscopic study of the interaction between human lymphocytes and streptococcal L-forms is suspensions has also been carried out. The red blood cells of all species under study, as well as human lymphocytes, have been found capable of being adsorbed on the colonies of streptococci in the L-form, this adsorption occurring only on the periphery of the colony. The study of electron-microscopic preparations has revealed that streptococcal L-forms adhere to the surface (on 1 or 2 sites) and to the processes of human lymphocytes.
