[MORPHOLOGICAL AND IMMUNOPHENOTYPE OF DESCRIPTION OF LIPOASPIRATE CELLS].

In an experimental investigation immunophenotype and morphological characteristics of cells isolated from adipose tissue were studied. It is establised that cells derived from lipoaspirate, namely the third subpopulation cells, characterized by the features inherent to mesenchymal stem cells (SC) and SC similar to those of bone marrow. The presence of cells isolated from lipoaspirate, features that characterize them as the SC, is the perspective of their use in clinical practice.

[Peculiarities of heterotopic transplantation of adipose graft enriched by allogeneic cryopreserved stem cells from adipose lipoaspirate].

Possibilities of application of the donor cryoconserved cellular material (mesenchymal stem cells, obtained from adipose tissue) for defense of transplanted adipose graft from the tissue resorption were studied. The adipose graft enrichment, using cryoconserved allogenic mesenchymal multipotent stem cells, obtained from adipose tissue, caused activation of destructive-inflammatory processes in adipose graft, the adipocytes survival worsening, and in further--the transplant mass deficiency. We suggest inexpedient to apply in clinical practice the donor cryoconserved stem cells for defense of transplanted adipose tissue from the tissue resorption.

[Transplantation of adipose tissue multipotent stromal cells in the treatment of chronic ischemia of the lower extremities].

The expediency of the stromal cells application, obtained from adipose tissue, was determined in a frame of preclinical investigations conduction, concerning experimental works on laboratory rats, in whom the extremity ischemia was simulated. Histologic and immunohistochemical changes were studied in muscular tissue after transplantation of multipotent stromal cells of own adipose tissue in patients, suffering ischemia of the lower extremity. Reduction of severity of the myofibrills ischemic damage, rapid activation of the muscles regenerative power, accurate stimulation of the angiogenesis processes in 3 mo after transplantation of the cells were demonstrated.

[Assurance of intestinal transplant quality].

Issues of preservation of function and the intestinal transplant quality, security for a recipient, prophylaxis of rejection reaction, immunosuppressive therapy were enlighted.

[Ultrasonic cleavage of DNA in complexes with Ag(I), Cu(II), Hg(II)].

We investigated a phenomenon of ultrasonic cleavage of DNA complexed with transition metal cations Ag(I), Cu(II) and Hg(II). We found the statistically significant dependence of relative intensity of cleavage on cation type and concentration. Each cation may cause two different types of distortion in the DNA double-helix depending on whether it binds to major or minor DNA groove. The intensity of ultrasonic cleavage decreases if cation binds to the major DNA groove; the intensity of cleavage increases if cation binds to the minor DNA groove and disturbs the hydrogen bonds of complementary base pairs or it intercalates between bases. Both types of DNA distortion can affect the intensity of N-S interconversion of deoxyribose.

[Heterogeneity of double-stranded DNA local structure and dynamics: ultrasound studies].

A method for studying the local inhomogeneities of DNA and its dynamics is proposed. The method is based on the combination of two procedures, splitting the DNA molecules by ultrasound and analysis of DNA fragments obtained by gel electrophoresis. The frequency of cleavage of internucleotide bonds was found to depend on the type of nucleotides forming the bond and on their nearest neighbors. Estimates of cleavage frequencies in each of 16 dinucleotides showed that, in the 5'-d(CpG)-3', 5'-d(CpA)-3', and 5'-d(CpT)-3' dimers, the cleavage occurs considerably more frequently than in the rest, and the frequency of cleavage depends on the nearest neighbors. It was shown that the double-helix cleavage can occur with shifts by several nucleotides. Physical prerequisites were considered that can lead to this pattern of sequence - specific cleavage.

[Effect of geomagnetic activity on the rhythm of resistance of rats to hypoxia]. / Vliianie geomagnitnoi aktivnosti na ritmy ustoichivosti krys k gipoksii.

The resistance of male Wistar rats to acute hypoxia was estimated from the lifetime at an "altitude" of 11.5 km above sea level from 13 to 21 p.m. and in different seasons of a year. Identical types of rhythms (circaseptan, circasemiseptan, infradian, circadian, and ultradian) of geomagnetic activity and lifetime were revealed. It was found that the periods of basal rhythms either coincide or are multiple. It was shown that the rhythms of geomagnetic activity (including nonbasal rhythms) affect lifetime rhythms (especially ultradian rhythms). As the periods of the rhythms decrease, the number of rhythms for both parameters increase (ultradian rhythms are most numerous), and relative differences in the values of periods (they are minimal for ultradian rhythms) and the amplitudes of rhythms decrease.

Seasonal variations in rat resistance to hypoxia.

Resistance to acute hypoxia was evaluated by the life span after elevation to a simulated altitude of 11,500 m at 13.00-21.00 in different seasons of the same year. The period of investigation was divided into 3 parts: beginning (13.00-15.00), middle (16.00-18.00), and end (19.00-21.00) of the day. Animal life span decreased by the end of the day (the decrease was more pronounced in summer and autumn) and by the end of the year, i. e. in autumn (more pronounced in the middle and end of the day). The life span was longer in the middle of the day in spring in low- and medium-resistant rats and by the end of the year in medium-resistant rats (at the beginning of the day). Fluctuations in the life span during the day and year were more expressed in low- and medium-resistant rats. Differences in the life span of highly and low-resistant rats were the most pronounced in winter.

Effect of geomagnetic activity on rat resistance to hypoxia.

Resistance to acute hypoxia in rats was evaluated by the life span after elevation to an altitude of 11,500 m at 13.00-21.00 (local time) in different seasons of one year. Geomagnetic activity was evaluated using local K index for Moscow and planetary Kp index. Total 24-h geomagnetic activity had a great impact on the life span of rats. The effects of local and planetary geomagnetic activities coincided in about 70% cases. An increase in geomagnetic activity was responsible for prolongation, decrease (2-3 times more often than prolongation), and phase changes in the life span of rats, which were the most pronounced in the case of medium geomagnetic activity, during the second half of the year (in summer and particularly in autumn), in the middle and end of the day, in rats with low resistance to hypoxia.

[Integral equations of the theory of liquid in the study of hydration of macromolecules]. / Integral'nye uravneniia teorii zhidkostei v issledovanii gidratatsii makromolekul.

An efficient numerical algorithm for solving integral equations of the theory of liquid in the RISM approximation for infinitely diluted solution of macromolecules with a large number of atoms is proposed. The algorithm is based on applying the non-stationary interactive methods for solving systems of linear algebraic equations. Using this technique we have calculated the solvent-solute atom-atom correlation functions for short fragment of DNA duplex of varying length in aqueous solution.

[Hydration of a fragment of the B-form of DNA duplex: d(GGGGG). A method of integral equations of the theory of liquid]. / Gidratatsiia fragmenta dupleksa B-formy DNK: d(GGGGG). Metod integral'nykh uravnenii teorii zhidkosti.

Based on the numerical algorithm proposed by us in [1] for solving integral equations of the theory of liquids in the RISM approximation we calculate all of the solvent-solute atom-atom correlation functions for a fragment of the DNA duplex d(GGGGG) in infinitely diluted aqueous solution. The obtained results are compared with available experimental data and results from computer simulations.

[The use of the fluorescent probe method for the diagnosis of acute coronavirus intestinal infections in people]. / Primenenie metoda fluorestsentnykh zondov dlia diagnostiki koronavirusnykh ostrykh kishechnykh infektsii u liudei.

The scientific investigations conducted first allow using a method of fluorescent probes for the diagnosis of human coronaviral intestinal infection. The method is characterised by sensitivity, specificity, reproductivity and proximity. It is comparable, according to these parameters, with the methods of the third generation and may be recommended for putting into practical work of virological laboratories.

[Optimizing conditions of cell culture use for virus indications by means of fluorescent probes]. / Optimizatsiia uslovii ispol'zovaniia kletochnykh kul'tur dlia indikatsii virusov s pomoshch'iu metoda fliuorestsentnykh zondov.

Cell cultures in a suspension on medium 199 with 1 x 10(6) cells per ml are recommended for virus indication by means of fluorescent probe method. When stored at 4 degrees C, the cells are fit for work for 5 days. The proposed conditions for the use of cell cultures are necessary for effective introduction of the fluorescent probe method in practical virological laboratories.

[The structure and immunobiological properties of coronavirus proteins]. / Struktura i immunobiologicheskie svoistva belkov koronavirusov.

The survey includes data concerning structural proteins of coronaviruses obtained for the last ten years. It elucidates such problems as the proteins organization in the virion, heterogeneity of their composition, structure and biological properties, antigenic and immunobiological properties, prospects of creation of specific immunogenic anticoronavirus, prophylactic and diagnostic preparations.

[Monitoring the health status in workers of chemical plants]. / Podkhody k monitoringu sostoianiia zdorovía rabochikh khimicheskikh proizvodstv.

A morbidity level of workers in chemical industry depends on labour conditions, anthropometric dates and harmful habits. As informative signs of health state are 2-6 indices of humoral and cellular immunity. The age, social state and life duration in this town are less informative for health state.

[The variability of the scabies mite Sarcoptes scabiei de Geer (Acariformes, Sarcoptidae) in relation to the epidemiology of scabies. 4. The variability of the mite population on a patient and its focal variability]. / Izmenchivost' chesotochnogo kleshcha Sarcoptes scabiei de Geer (Acariformes, Sarcoptidae) v sviazi s épidemiologiei chesotki. Soobshchenie 4. Izmenchivost' populiatsii kleshchei na bol'nom i ochagovaia izmenchivost'.

The authors analyze the individual variability of the agent of scabies in populations on individual patients and in foci of the disease. They define an elementary micropopulation and a focal population notions. A total of 763 females, collected from the hands of 100 patients with scabies were examined. The findings evidence that the degree of the chetoid covering joining in the mite females, assessed in grades, may be regarded as an indicator of the population status in the pathologic process. Grades II and III females predominate in the mite population on the patients during the initial period of the disease (up to a month), whereas in the course of a full-bloom process (over a month) Grades I and 0 females prevail. The attendant exo- and endogenous factors shift the grade composition of the females towards the higher grades whatever the length of the disease. A great variety of the females in focal populations evidences reinfection and continuity of the process.