ABSTRACT
OBJECTIVE: To investigate the aminoacid composition in the stromal fibroblasts of bone marrow and indexes of hemogram in children with acute lymphoblastic leukemias, which exposed to radiation from the Chornobyl accident, for evaluation of the median survival. MATERIALS AND METHODS: In the study 54 patients with acute lymphoblastic leukemia, residents of Kyiv, Zhytomyr and Chernihiv regions of Ukraine were involved. Effectiveness of stromal fibroblasts colony forming units of bone marrow and aminoacid composition of fibroblasts were studied. Bone marrow examination was conducted on day33 and after stop of chemotherapy, the median survival of patients and exposure dose were evaluated. RESULTS: In children with acute leukemias effectiveness of stromal fibroblasts of bone marrow was 2-2,3 times lower than normative. Correlation (rs = +0,83) between the number of erythroid progenitor cells in the bone marrow and proline content in stromal fibroblasts established . The correlation between patients median survival and number of granulocytes in myelogram (rs = + 0,82) was proven. Mean exposure doses in patients was (5,86 ± 1,11) mSv and it did not correlate with myelogram results, effectiveness of stromal fibroblasts of bone marrow and median survival. CONCLUSIONS: The median survival of patients with acute leukemias depends on the content of amino acids in stro mal fibroblasts of bone marrow and recover of hematopoiesis after chemotherapy.
ABSTRACT
The authors present an assessment of bone structure condition in children with acute leukemia. The changes in the collagen molecule, amino acid composition of urine and proteins reparation processes were revealed. Calcium phosphate excretion in the patients urine were increased. The serum osteocalcin level and colony formation efficiency of bone marrow fibroblasts in acute leukemia patients are lower than in control group. In the initial period of the disease 32% of patients have disturbancies in their endocrine status. The bone structure violation is combined with unfavorable disease outcome. The effectiveness of the treatment and prevention steps in acute leukemia patients depends on the leukemic process stage.
Subject(s)
Amino Acids , Bone and Bones/pathology , Collagen/chemistry , Gamma Rays/adverse effects , Leukemia , Acute Disease , Adolescent , Amino Acids/blood , Amino Acids/urine , Bone Marrow/metabolism , Bone Marrow/pathology , Bone and Bones/metabolism , Bone and Bones/radiation effects , Calcium Phosphates/urine , Case-Control Studies , Chernobyl Nuclear Accident , Child , Child, Preschool , Collagen/metabolism , Colony-Forming Units Assay , Female , Fibroblasts/metabolism , Fibroblasts/pathology , Humans , Infant , Leukemia/blood , Leukemia/etiology , Leukemia/pathology , Leukemia/urine , Male , Neoplasm Staging , Osteocalcin/urine , Radiation Dosage , UkraineABSTRACT
On the basis of complex approach to bone and haematopoetic tissue interaction the authors studied the influence of low weight metabolites on stromal fibroblasts and components of extacellular matrix of bone and skin (collagen and glycosaminoglycans). Specificity of different metabolites action on physico-chemical abilities of type I collagen, amino acid composition changes, surface charge, ratio of alpha- and beta-compounds, BrCN-fragments of alpha-1 component cross-links was shown. The dose dependence of formiate effect on processes of proteins glycosilation, cross-linking in bone and cartilage connective tissue and serum glycoproteins was established. The results obtained showed sensitivity of the bone tissue exstacellular matrix to influence of low-weight metabolites action at the level of post-synthetic modification of its components, their intermolecular interaction and process of osteogenesis.
Subject(s)
Amino Acids/metabolism , Connective Tissue/metabolism , Extracellular Matrix/metabolism , Stromal Cells/metabolism , Animals , Bone Marrow Cells/metabolism , Bone and Bones/cytology , Bone and Bones/drug effects , Bone and Bones/metabolism , Cells, Cultured , Collagen Type I/metabolism , Connective Tissue/drug effects , Fibroblasts/metabolism , Formates/pharmacology , Glycoproteins/blood , Glycosaminoglycans/metabolism , Humans , Leukemia/metabolism , Male , Mice , Mice, Inbred Strains , Osteogenesis/physiology , Rats , Rats, Wistar , Skin/cytology , Skin/drug effects , Skin/metabolism , Stromal Cells/cytology , Stromal Cells/drug effectsABSTRACT
The artificial mountain air influence on immunity indices of orthopedic and traumatologic patients with immunodepression was studied. The positive effect of interrupted normobaric hypoxitherapy on the immune state of patients was shown by means of increase of stromal elements of bone marrow, developing microsurroundings for maturation of immunocompetent cells. It was suggested to apply orotherapy in the preoperative preparation complex of orthopedic and traumatologic patients with immunodepression.
Subject(s)
Air/analysis , Bone Diseases/immunology , Hypoxia/therapy , Wounds and Injuries/immunology , Atmospheric Pressure , B-Lymphocytes/immunology , Bone Diseases/pathology , Bone Marrow/immunology , Humans , Hypoxia/physiopathology , Immune Tolerance , Immunocompromised Host , Male , T-Lymphocytes/immunology , Wounds and Injuries/pathologyABSTRACT
Basing on cloning in vitro, colony-forming stromal cells (CFSC) of the bone marrow were studied in 66 children with a congenital dislocation of the hip. Degenerative processes in the bones of the hip joint were followed by a significant decrease of CFSC cloning. A direct correlation between the results of surgical treatment and cloning efficiency is established. Thus, the cloning efficiency may be used as a criterion for determining feasibility, time of surgery and its prognosis.
