3-Polythiophene Acetic Acid Nanosphere Anchored Few-Layer Graphene Nanocomposites for Label-Free Electrochemical Immunosensing of Liver Cancer Biomarker.

This study devised a label-free electrochemical immunosensor for the quantitative detection of alpha-fetoprotein (AFP). 3-Polythiophene acetic acid (3-PTAA) nanoparticles were anchored onto a few-layer graphene (FLG) nanosheet, and the resulting nanocomposite was utilized as the immunosensor platform. The AFP antibody (anti-AFP) was immobilized on 3-PTAA@FLG via a covalent interaction between the amine group of anti-AFP and the carboxylic group of 3-PTAA via ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC)/N-hydroxysuccinimide (NHS) coupling. FLG is largely responsible for providing electrochemical signals, whereas 3-PTAA nanoparticles are well-known for their ability to be compatible with biological molecules in neutral aqueous solutions. Moreover, the carboxyl group present in 3-PTAA effectively binds anti-AFP through EDC/NHS conjugation. Owing to good dispersibility and higher surface area of 3-PTAA, it is very convenient for casting the polymer directly on the electrode substrate followed by immobilization of anti-AFP. Thus, it is feasible to regulate the activity of AFP proteins and control the spatial distribution of the immobilized anti-AFP proteins. The electrochemical sensing performance was assessed via cyclic voltammetry and electrochemical impedance spectroscopy. For an increase in the bioconjugate concentration, the results demonstrated a surge in charge-transfer resistance and a consequent decline in the current response. This approach effectively detected AFP at an extended dynamic range of 0.0001-250 ng/mL with a detection limit of 0.047 pg/mL. Furthermore, the sensing capacity of the immunosensor for AFP detection has been demonstrated to be steady in real human serum cultures. Our approach exhibits good electrochemical performance in terms of reproducibility, selectivity, and stability, which would surely impart budding applications in the clinical diagnosis of several other tumor markers.

Using a Self-Assembled Two-Dimensional MXene-Based Catalyst (2D-Ni@Ti3C2) to Enhance Hydrogen Storage Properties of MgH2.

In this work, we report the remarkable catalytic effects of a novel Ti3C2 MXene-based catalyst (Ni@Ti-MX), which was prepared via self-assembling of Ni nanoparticles onto the surface of exfoliated Ti3C2 nanosheets. The resultant Ni@Ti-MX catalyst, characterized by ultradispersed Ni nanoparticles being anchored on the monolayer Ti3C2 flakes, was introduced into MgH2 through ball milling. In situ transmission electron microscopy (TEM) analysis revealed that a synergetic catalytic effect of multiphase components (Mg2Ni, TiO2, metallic Ti, etc.) derived in the MgH2 + Ni@Ti-MX composite exhibits remarkable improvements in the hydrogen sorption kinetics of MgH2. In particular, the MgH2 + Ni@Ti-MX composite can absorb 5.4 wt % H2 in 25 s at 125 °C and release 5.2 wt % H2 in 15 min at 250 °C. Interestingly, it can uptake 4 wt % H2 in 5 h even at room temperature. Furthermore, the dehydrogenation peak temperature of the MgH2 + Ni@Ti-MX composite is about 221 °C, which is 50 and 122 °C lower than that of MgH2 + Ti-MX and MgH2, respectively. The excellent hydrogen sorption properties of the MgH2 + Ni@Ti-MX composite are primarily attributed to the peculiar core-shell nanostructured MgH2@Mg2NiH4 hybrid materials and the interfacial coupling effects from different catalyst-matrix interfaces. The results obtained in this study demonstrate that using self-assembling of transition-metal elements on two-dimensional (2D) materials as a catalyst is a promising approach to enhance the hydrogen storage properties of MgH2.

Experimental and numerical investigation of a polypropylene orthotic device for assistance in level ground walking.

This study investigates the use of an orthotic device for improving pathologic gait lacking a heel-strike and its effect on the joint loads. The orthosis is fabricated from 10-mm thick polypropylene sheets joined together using a bolted joint. The gait trials are recorded using a Qualisys motion capture system and Kistler's force platform. The data recorded in this study comprise five male and five female participants, executing level ground gait under barefoot, shod and orthotic conditions. Computed tomography reconstructed foot bone-tissue model and computer-aided design model of the orthosis are used to predict the mechanical behaviour with and without orthosis under static loading. A one-way analysis of variance is conducted to compare the peak gait parameters in the early and late stance phase between the three walking conditions. The experimental results show that the orthosis reduces the peak joint forces and the rate of change of moment at the hip, knee and ankle joints. The finite element analysis results present a decrease in foot plantar pressure from 0.74 to 0.32 MPa with orthotic usage. The results of this study indicate that the orthosis can eliminate the heel-ground gap while retaining sufficient ankle motion and providing peak joint force reduction.

Effect of high heel gait on hip and knee-ankle-foot rollover characteristics while walking over inclined surfaces - A pilot study.

Given the massive number of individuals wearing high-heeled shoes, understanding the gait biomechanics associated with their use could provide insight into clinically preventable abnormalities. The effects of inclined surfaces on the high-heeled gait have been investigated in the present pilot study, as most walking surfaces encountered in routine life are rarely perfectly level grounded. The rollover shapes of the high-heel shod gait are calculated to obtain the desired results. An adjustable inclined walkway setup was fabricated and comprising fixed slots permitting discrete and variable angle of inclinations (≤30°). The gait trials were recorded for the heel shod walking of ten healthy female volunteers using the three-dimensional motion analysis system by varying the inclination of the fabricated walkway. From the calculated rollover shapes, the necessary radii of the hip and the knee-ankle- foot rollover shapes were obtained and a repeated measures analysis of variance was carried out to establish the existence of correlation between the angle of inclination and rollover radii. The results of the present pilot study show that for high heel-shod walking there exist variable radii of curvature for early and late stance phases and that the same may vary depending upon the inclination. The same information can be used to modify the design of high-heel shoes to improve the stability while retaining their aesthetics.

Effect of Strain Heterogeneities on Microstructure, Texture, Hardness, and H-Activation of High-Pressure Torsion Mg Consolidated from Different Powders.

Severe plastic deformation techniques, such as high-pressure torsion (HPT), have been increasingly applied on powder materials to consolidate bulk nanostructured materials. In this context, the aim of the present study is to compare the plastic deformation characteristics during HPT of two distinct Mg-based powder precursors: (i) atomized micro-sized powder and (ii) condensed and passivated nanopowder. Dynamic recrystallization could take place during HPT consolidation of the atomized powder particles while the oxide pinning of the grain boundaries restricted it for the condensed powder. Consequently, there have been substantial differences in the development of the microstructure, texture, local strain heterogeneities, and hardness in the two types of consolidated products. Different types of local strain heterogeneities were also revealed in the consolidated products. The associated diversity in microstructure within the same consolidated product has been demonstrated to have an effect on the hydrogen activation kinetics to form hydrides for these Mg-based materials that could be suitable for solid state H-storage applications.