Lead Isotope Evidence for Enhanced Anthropogenic Particle Transport to the Himalayas during Summer Months.

The Indo-Gangetic Plain (IGP) is one of the most highly polluted regions of the world, yet the temporal pattern of transport of anthropogenic aerosols from this region to the Himalayas is poorly constrained. On the basis of the seasonal variation of planetary boundary layer heights, air mass back trajectory analysis, and year-long time-series data for 208Pb/204Pb, 207Pb/204Pb, 206Pb/204Pb, and 143Nd/144Nd from aerosols collected over a high-altitude station, we demonstrate that anthropogenic Pb transport to the glacierized catchment has a seasonal pattern. The Pb isotope data reveal that during winter, the thinned boundary layer traps up to 10 ± 7% more coal-derived Pb in the IGP. In contrast, in nonwinter months, a thicker boundary layer and enhanced subtropical westerly winds result in efficient Pb transport to the Himalayas. As Pb isotope ratios are robust conservative chemical tracers and Pb is predominantly derived from anthropogenic sources, these observations suggest that enhanced transport of anthropogenic aerosols to the glacierized catchment of the Himalayas coincides with higher near-surface temperatures in the summer, creating positive feedback that enhances melting. Our results further suggest that >50% of Pb in the Himalayan aerosols originates from the resuspension of historic Pb derived from phased out leaded gasoline, highlighting the importance of legacy Pb stored in the Indo-Gangetic Plains.

Biomass-Derived Provenance Dominates Glacial Surface Organic Carbon in the Western Himalaya.

The origin, transport pathway, and spatial variability of total organic carbon (OC) in the western Himalayan glaciers are poorly understood compared to those of black carbon (BC) and dust, but it is critically important to evaluate the climatic role of OC in the region. By applying the distribution of OC activation energy; 14C activity; and radiogenic isotopes of 208Pb/204Pb, 207Pb/204Pb, and 206Pb/204Pb in glacial debris and atmospheric particulate matter (PM10 size fraction), we demonstrate that 98.3 ± 1.6 and 1.7 ± 1.6% of OC in western Himalayan glaciers are derived from biomass and petrogenic sources, respectively. The δ13C and N/C composition indicates that the biomass is a complex mixture of C3 vegetation and autochthonous photoautotrophic input modified by heterotrophic microbial activity. The data set reveals that the studied western Himalayan glacier has negligible contributions from fossil-fuel-derived particles, which contrasts to the central and eastern Himalayan glaciers that have significant contributions from fossil fuel sources. We show that this spatial variability of OC sources relates to regional differences in air mass transport pathways and precipitation regimes over the Himalaya. Moreover, our observation suggests that biomass-derived carbon could be the only primary driver of carbon-induced glacier melting in the western Himalaya.

Rv1273c, an ABC transporter of Mycobacterium tuberculosis promotes mycobacterial intracellular survival within macrophages via modulating the host cell immune response.

Mycobacterium proteins, especially cell wall associated proteins, interact with host macrophage to regulate the functions and cytokine production. So, identification and characterization of such proteins is essential for understanding tuberculosis pathogenesis. The role of the ABC transporter proteins in the pathophysiology and virulence of Mycobacterium tuberculosis is not clearly understood. In the present study, Rv1273c, an ABC transporter, has been expressed in a non-pathogenic and fast growing Mycobacterium smegmatis strain to explore its role in host pathogen interactions. Over expression of Rv1273c resulted in enhanced intracellular survival in macrophage as well as modified cell wall architecture. We found altered colony morphology and cell surface properties that might be linked with remodelling of bacterial cell wall which may help in the intracellular survival of mycobacterium. However, the enhanced intracellular survival was not found to be the consequence of an increased resistance to intracellular stresses. The activation of macrophage by Rv1273c was associated with perturbed cytokine production. Pharmacological inhibition experiment and western immunoblotting suggested that this altered cytokine profile was mediated possibly by NF-kB and p38 pathway in macrophage. Overall, the present findings indicated that Rv1273c enhanced mycobacterium persistence and mediated the evasion of immune responses during infection.

Declining pre-monsoon dust loading over South Asia: Signature of a changing regional climate.

Desert dust over the Indian region during pre-monsoon season is known to strengthen monsoon circulation, by modulating rainfall through the elevated heat pump (EHP) mechanism. In this context, an insight into long term trends of dust loading over this region is of significant importance in understanding monsoon variability. In this study, using long term (2000 to 2015) aerosol measurements from multiple satellites, ground stations and model based reanalysis, we show that dust loading in the atmosphere has decreased by 10 to 20% during the pre-monsoon season with respect to start of this century. Our analysis reveals that this decrease is a result of increasing pre-monsoon rainfall that in turn increases (decreases) wet scavenging (dust emissions) and slowing circulation pattern over the Northwestern part of the sub-continent.