ABSTRACT
Cell-free protein synthesis (CFPS) has emerged as a powerful tool for the rapid synthesis and analysis of various structurally and functionally distinct proteins. These include 'difficult-to-express' membrane proteins such as large multipass ion channel receptors. Owing to their membrane localization, eukaryotic CFPS supplemented with endoplasmic reticulum (ER)-derived microsomal vesicles has proven to be an efficient system for the synthesis of functional membrane proteins. Here we demonstrate the applicability of the eukaryotic cell-free systems based on lysates from the mammalian Chinese Hamster Ovary (CHO) and insect Spodoptera frugiperda (Sf21) cells. We demonstrate the efficiency of the systems in the de novo cell-free synthesis of the human cardiac ion channels: ether-a-go-go potassium channel (hERG) KV11.1 and the voltage-gated sodium channel hNaV1.5.
Subject(s)
Ether-A-Go-Go Potassium Channels , Heart , Animals , Cricetinae , Humans , Ether-A-Go-Go Potassium Channels/genetics , CHO Cells , Cricetulus , Membrane ProteinsABSTRACT
Here, we demonstrate the utility of native membrane derived vesicles (nMVs) as tools for expeditious electrophysiological analysis of membrane proteins. We used a cell-free (CF) and a cell-based (CB) approach for preparing protein-enriched nMVs. We utilized the Chinese Hamster Ovary (CHO) lysate-based cell-free protein synthesis (CFPS) system to enrich ER-derived microsomes in the lysate with the primary human cardiac voltage-gated sodium channel 1.5 (hNaV1.5; SCN5A) in 3 h. Subsequently, CB-nMVs were isolated from fractions of nitrogen-cavitated CHO cells overexpressing the hNaV1.5. In an integrative approach, nMVs were micro-transplanted into Xenopus laevis oocytes. CB-nMVs expressed native lidocaine-sensitive hNaV1.5 currents within 24 h; CF-nMVs did not elicit any response. Both the CB- and CF-nMV preparations evoked single-channel activity on the planar lipid bilayer while retaining sensitivity to lidocaine application. Our findings suggest a high usability of the quick-synthesis CF-nMVs and maintenance-free CB-nMVs as ready-to-use tools for in-vitro analysis of electrogenic membrane proteins and large, voltage-gated ion channels.
Subject(s)
Voltage-Gated Sodium Channels , Cricetinae , Animals , Humans , Cricetulus , CHO Cells , Membrane Proteins , LidocaineABSTRACT
The aim of the present study was to evaluate the expression and localization of lymphangiogenic factors (VEGF-C and VEGF-D), their receptor (VEGFR3) and lymphatic endothelial marker (LYVE1) in buffalo placenta during early pregnancy [EP], and to investigate the functional role of lymphangiogenic growth factors in placental lymphangiogenesis. The mRNA and protein expression of VEGF-C, VEGF-D, their receptor VEGFR3 and LYVE1 showed significant expression in EP1 (29-42 days) and EP2 stages (51-82 days) both in caruncle (maternal part) and cotyledon (foetal part) of the buffalo placenta. Immunoreactivity of VEGF-C, VEGF-D and LYVE1 was observed around the endometrial gland, in lymphatics and trophoblast cells, whereas VEGFR3 mainly localized in lymphatics of the caruncle and cotyledons. Cultured trophoblast cells were treated with VEGF-C/VEGF-D (50, 100 and 150 ng/ml) and combined doses of VEGF-C and VEGF-D (150 ng/ml) each for different time durations (24, 48 and 72 h). The mRNA expression of LYVE1 and PCNA was significantly (p < .001) upregulated with VEGF-C and VEGF-D and combined treatment (@150 ng/ml), as well as significantly downregulating Caspase-3 at 48 and 72 h. Thus, the present study provides evidence that lymphangiogenic factors are expressed in buffalo placental compartments and they may play a significant role in the regulation of placental function in water buffaloes.
Subject(s)
Lymphangiogenesis , Vascular Endothelial Growth Factor C , Female , Pregnancy , Animals , Lymphangiogenesis/genetics , Vascular Endothelial Growth Factor C/genetics , Vascular Endothelial Growth Factor C/metabolism , Placenta/metabolism , Vascular Endothelial Growth Factor D/genetics , Vascular Endothelial Growth Factor D/metabolism , Buffaloes/genetics , RNA, Messenger/genetics , RNA, Messenger/metabolismABSTRACT
The adsorption of thymine, a key pyrimidine base of deoxyribonucleic acid (DNA) on montmorillonite clay (Mnt) exchanged with metal ions (Mnt-M2+, M2+ = Fe2+, Co2+, Cu2+, Ca2+, and Mg2+) over a range of concentration (7.0 × 10-5 M to 12.0 × 10-5 M) and pH (4.0 - 9.0) at ambient temperature has been investigated in aqueous environment spectrophotometrically (UV, FTIR, XRD, SEM-EDX). The effectiveness of various adsorbents was determined in terms of percent (%) binding and Langmuir constants (KL and Xm) using Langmuir adsorption isotherm at their respective pH of maximum adsorption. Transition metal ions incorporated Mnt, particularly Fe2+ have shown better adsorption ability than alkaline earth metal ions. The present study reveals the significant role of divalent metal cation exchanged Mnt clay in the chemical evolution of biomolecules of genetic continuity and self-replication which might have occurred through the adsorption of thymine on and between their silicate layers to protect and achieve biocompatibility.
Subject(s)
Bentonite , Evolution, Chemical , Clay/chemistry , Bentonite/chemistry , Adsorption , Thymine , Prebiotics , Cations/chemistry , Cations, Divalent , Metals , Hydrogen-Ion Concentration , KineticsABSTRACT
Periparturient dairy cows and their newborn calves are highly prone to health complications. Enhancing the innate immune system of these animals is essential to mitigate the transition period stress and promote their health. Macrophage activating factor (MAF) possess immunomodulatory properties and is believed to enhance immune response. In the present study, the impact of different concentrations (10, 50, 100 ng) of MAF on the phagocytic activity (PA) of murine and bovine phagocytoses was explored. MAF synthesized from IgA of cow colostrum was studied for its effect on the phagocytic index (PI) of cow colostrum macrophages (Mφ) and blood neutrophils (sick and healthy calves) under in vitro conditions. Besides, the impact of MAF on the PI of peritoneal Mφ of healthy and immunocompromised mice was studied. PI of healthy Mφ (mice peritoneal and cow colostrum) and healthy neutrophils (blood calf) increased significantly (P < 0.05) after MAF supplementation. MAF also significantly (P < 0.05) increased the PI of neutrophils and Mφ obtained from sick calf and immunocompromised mice, respectively. Results indicate that colostrum MAF can be used as a potential immune modulator to promote immunity and fight infections in dairy animals.
Subject(s)
Colostrum , Phagocytes , Animals , Cattle , Female , Immunoglobulin A , Macrophage-Activating Factors , Macrophages , Mice , PregnancyABSTRACT
Cancer is amongst the leading public health problems globally with continuously increasing prevalence rate that demands for extensive and expensive treatment. Despite availability of number of potential cancer therapies, inadequate success has been achieved due to complexity and heterogeneity of tumors. Moreover, late/ terminal stage cancer leads to multidrug resistance, excruciating side effects, recurrence, etc. This is because of low penetrability and deleterious effects of drug on non-target cells/ tissues. This requires for cost effective, efficacious, alternative/ adjunct, complementary medicines with targeted drug delivery approach. A potential strategy to resolve this difficulty is to use theranostics i.e., formulations having both a therapeutic element and an imaging agent. Phytotherapeutics have been extensively used since times immemorial, having wide acceptability, easy availability, minimal side effects and comparatively inexpensive. These herbal formulations are mostly orally administered and thus subjected to adverse pH, enzymatic degradation, poor gut absorption, low bioavailability and non-targeted delivery that ultimately lead to their poor effectiveness. Constraints associated with conventional phyto-pharmaceuticals can be improved by designing and using "Nano Delivery Systems" (NDS). The foremost aim of metal based NDS is to provide sustained drug release, site-specific action, improved patient's compliance and enhanced efficacy. Metal Nanocarriers carrying herbal drugs will avoid these obstructions, so the drug can circulate into the blood for a longer period of time and provide optimal amount of the drug to the site of action. Besides, herbal drugs with NDS thus would be efficacious as alternative/ complementary cancer theranostics. Present review describes novel theranostic systems employing metal nanocarriers with diagnostic and therapeutic properties as an effective strategy for cancer treatment. These systems when conjugated with herbal drugs provide an efficient management strategy for cancer.
Subject(s)
Neoplasms , Precision Medicine , Drug Delivery Systems , Drug Liberation , Humans , Nanotechnology , Neoplasms/drug therapyABSTRACT
BACKGROUND: Periparturient period induces stress in cows which fluctuates hormonal and metabolic function and causes immune suppression. Apart from impairing the health, production, and reproduction of cows, it also influences the well-being of newborn calves by decreasing the colostrum quality. Micronutrients are known for optimal health and production and their effects on parturition stress, immune response in both cow and its calf need to be explored. AIM: The aim of this study was to see the effect of oral supplementation of micronutrients during the prepartum period on the health status of crossbred dairy cows and subsequently on their newborn calves. METHODS: A total of 42â¯healthy multiparous cows were selected and randomly divided into five groups with seven cows in each group, i.e. control (Basal Diet, BD), VA group (BDâ¯+â¯vitamin A, 105 IU), Zn group (BDâ¯+â¯zinc sulphate, 60â¯ppm), VE group (BDâ¯+â¯vitamin E, 2500 IU), and combined supplementation (CS) group (BDâ¯+â¯combination of VA, Zn, and VE). The supplements were offered in compounded concentrate DM (100â¯g) to individual cows once daily before the morning feeding and the remaining portion was incorporated in the TMR. Feeding was started one month before the expected days of calving till calving. Blood samples were collected from cows at days -15, -7, -3, 0, +3, +7, and +15 relative to the day of calving. Blood samples from newborn calves and milk samples of cows were collected at days 0, +3, +7, and +15. Milk somatic cell counts (SCC) were estimated using a cell counter. Cortisol was estimated by ELISA kit in blood and milk plasma of cows and in the blood plasma of their calves. Total immunoglobulins (Ig) were estimated in milk of cows and serum of calves using zinc sulphate turbidity method. Blood neutrophils from cows and calves were studied for phagocytic activity (PA) using nitro blue tetrazolium (NBT) assay.Data were analysed by repeated-measures two-way ANOVA using the mixed procedure of SAS, and the pairwise comparison was performed using a multiple comparison test (Tukey). RESULTS: Combined supplementation of micronutrients decreased (Pâ¯<â¯0.05) maternal blood plasma (control vs. CS group, 5.98⯱â¯0.20 vs. 3.86⯱â¯0.23â¯ng/mL) and milk plasma (3.96⯱â¯0.13 vs. 2.71⯱â¯0.10â¯ng/mL) cortisol, milk SCC (3.05⯱â¯0.11 vs. 2.12⯱â¯0.10â¯×â¯105 cells/mL) and increased (Pâ¯<â¯0.05) total milk Ig concentration (18.80⯱â¯0.11 vs. 23.04⯱â¯0.57â¯mg/mL) and the PA of blood neutrophils (0.84⯱â¯0.03 vs. 1.07⯱â¯0.03). Similarly, lower blood cortisol concentration (9.69⯱â¯0.35 vs. 6.02⯱â¯0.18â¯ng/mL) and higher (Pâ¯<â¯0.05) total Ig (23.26⯱â¯0.11 vs. 30.34⯱â¯0.70â¯mg/mL) and PA of blood neutrophils (0.37⯱â¯0.02 vs. 0.52⯱â¯0.02) were observed in the calves born to CS group of cows as compared to the control. Highest (Pâ¯<â¯0.05) positive effects (lower stress levels and higher immune response) of treatment were noticed in CS group followed by VE group and then Zn group. However, VA group didn't differ from the control group. CONCLUSION: Our results indicate that micronutrient interventions during the prepartum period can improve the health status of dairy calves and subsequently the well-being of their calves.
Subject(s)
Antioxidants/pharmacology , Immunoglobulins/immunology , Micronutrients/pharmacology , Zinc/immunology , Administration, Oral , Animals , Animals, Newborn , Antioxidants/administration & dosage , Cattle , Dietary Supplements , Immunoglobulins/blood , Micronutrients/administration & dosage , Oxidative Stress/drug effects , Zinc/bloodABSTRACT
AIMS AND OBJECTIVES: An efficient and facile DBU catalysed synthesis of highly significant motif 5,7-disubstituted-1,2,4-triazolo[1,5-a]pyrimidines under solvent-free condition has been reported. MATERIALS AND METHODS: To a round bottom flask, 1.0 mmol of chalcone (1), 1.5 mmol of 3-amino-1,2,4- triazole (2) and 30 mol% of DBU were added at 70 °C and stirred in solvent-free condition. After the completion of the reaction (monitored by TLC), water (10 ml) was added. The aqueous layer was extracted with ethyl acetate (3 ×10 ml). The combined organic layers were dried over anhydrous Na2SO4. The combined organic layers were evaporated under reduced pressure and the resulting crude product was purified by column chromatography by using ethyl acetate and hexane as eluent. RESULTS: Reaction using chalcone and 3-amino-1,2,4-triazole as model substrates were carried out under different reaction conditions and it was observed that 30 mol% of DBU under the solvent-free condition at 70 °C was the optimum temperature for the proposed synthesis. CONCLUSION: Use of DBU (an organocatalyst) as a base, operational simplicity, high yield of products and short reaction time are some of the significant advantages associated with the proposed strategy.
ABSTRACT
The objective of this study was to document the expression and functional role of BMPs in the placental (caruncle; CAR, cotyledon; COT) during different stages of pregnancy in water buffalo. Samples collected from Early pregnancy 1 (EP1); Early pregnancy 2 (EP2), Mid pregnancy (MP), Late pregnancy (LP) while the third stage of oestrus cycle (NP) was taken as control. Also, the synergistic role of BMP4/BMP7 or combination on mRNA expression of vWF, PCNA, StAR, CYP11A1, 3ßHSD, and BAX were studied in trophoblast cells cultured (TCC) during an early stage. The qPCR and immunoblotting studies revealed that BMP2, BMPR1A, BMPR1B, and BMPR2 mRNA level was significantly (pâ¯<â¯0.05) upregulated during early pregnancy in COTs while in CARs it was significantly upregulated (pâ¯<â¯0.05) during all the stages of pregnancy.BMP4 mRNA level was significantly upregulated (pâ¯<â¯0.05) during early pregnancy in COTs as well as in CARs. BMP6 expression was significantly upregulated (pâ¯<â¯0.05) during early and late stages of pregnancy. BMP7 mRNA level was upregulated (pâ¯<â¯0.05) during the late stage of pregnancy in COTs. At 100â¯ng/ml, the BMP4 maximally stimulated the transcripts of StAR, CYP11A1, and 3ßHSD while BMP7 maximally stimulated the transcripts of 3ßHSD that paralleled with P4 accretion in the media (Pâ¯<â¯0.05). BMP4 as well as BMP7 upregulated the transcripts of PCNA, vWF, and downregulated BAX in the TCC (Pâ¯<â¯0.05). In conclusion, BMPs are expressed in a regulated manner with stage-specific differences in the placenta and promotes the angiogenesis, proliferation, cell survivability, and steroidogenesis thereby regulating placental function in an autocrine/paracrine manner in water buffalo.
Subject(s)
Bone Morphogenetic Proteins/genetics , Buffaloes/genetics , Gene Expression Regulation, Developmental , Placenta/metabolism , Animals , Bone Morphogenetic Proteins/metabolism , Cell Survival , Female , Neovascularization, Physiologic/genetics , Placenta/blood supply , Pregnancy , RNA, Messenger/genetics , RNA, Messenger/metabolism , Time Factors , Trophoblasts/cytologyABSTRACT
Cellular calcium elevation is an important signal used by plants for recognition and signaling of environmental stress. Perception of the generalist insect, Spodoptera litura, by Arabidopsis (Arabidopsis thaliana) activates cytosolic Ca2+ elevation, which triggers downstream defense. However, not all the Ca2+ channels generating the signal have been identified, nor are their modes of action known. We report on a rapidly activated, leaf vasculature- and plasma membrane-localized, CYCLIC NUCLEOTIDE GATED CHANNEL19 (CNGC19), which activates herbivory-induced Ca2+ flux and plant defense. Loss of CNGC19 function results in decreased herbivory defense. The cngc19 mutant shows aberrant and attenuated intravascular Ca2+ fluxes. CNGC19 is a Ca2+-permeable channel, as hyperpolarization of CNGC19-expressing Xenopus oocytes in the presence of both cyclic adenosine monophosphate and Ca2+ results in Ca2+ influx. Breakdown of Ca2+-based defense in cngc19 mutants leads to a decrease in herbivory-induced jasmonoyl-l-isoleucine biosynthesis and expression of JA responsive genes. The cngc19 mutants are deficient in aliphatic glucosinolate accumulation and hyperaccumulate its precursor, methionine. CNGC19 modulates aliphatic glucosinolate biosynthesis in tandem with BRANCHED-CHAIN AMINO ACID TRANSAMINASE4, which is involved in the chain elongation pathway of Met-derived glucosinolates. Furthermore, CNGC19 interacts with herbivory-induced CALMODULIN2 in planta. Together, our work reveals a key mechanistic role for the Ca2+ channel CNGC19 in the recognition of herbivory and the activation of defense signaling.
Subject(s)
Arabidopsis Proteins/metabolism , Arabidopsis/metabolism , Arabidopsis/parasitology , Calcium Channels/metabolism , Cyclic Nucleotide-Gated Cation Channels/metabolism , Herbivory/physiology , Spodoptera/physiology , Animals , Arabidopsis/drug effects , Arabidopsis/genetics , Arabidopsis Proteins/genetics , Calcium/metabolism , Calcium Channels/genetics , Calcium Signaling/drug effects , Cell Membrane/drug effects , Cell Membrane/metabolism , Cell Membrane Permeability/drug effects , Cyclic Nucleotide-Gated Cation Channels/genetics , Cyclopentanes/pharmacology , Cytosol/drug effects , Cytosol/metabolism , Down-Regulation/drug effects , Gene Expression Regulation, Plant/drug effects , Glucosinolates/metabolism , Herbivory/drug effects , Methionine/metabolism , Models, Biological , Mutation/genetics , Oxylipins/pharmacology , Plant Leaves/drug effects , Plant Leaves/parasitology , Plant Vascular Bundle/drug effects , Plant Vascular Bundle/genetics , Protein Binding/drug effects , RNA, Messenger/genetics , RNA, Messenger/metabolism , Signal Transduction/drug effects , Spodoptera/drug effects , XenopusABSTRACT
INTRODUCTION: Cervical spondylosis is a general term for age-related wear and tear affecting the spinal disks in the neck. Keeping in view the increasing incidence of this problem in modern society a study was conducted with following. AIMS AND OBJECTIVES: The aim of the following study is to assess the efficacy of Griva Vasti in the management of cervical spondylosis. MATERIALS AND METHODS: The study was conducted on 10 clinically, pathologically, and radiologically diagnosed patients of cervical spondylosis, selected from outpatient department/In -patient department wing of P.G. Department of Kaya Chikitsa, N.I.A., Jaipur, satisfying the inclusion criteria. Patients were treated with Griva Vasti with Dasamula Tailam for 35-45 min duration, for 14 days. Assessments were performed with regard to neck disability index before and after treatment. STATISTICAL ANALYSIS: Statistical analysis was done with the help of Instat GraphPad 3 using Wilcoxon matched-pairs signed ranks test. RESULT: Relief in neck disability index was 65.70%, which was statistically significant (P < 0.01). CONCLUSION: Result indicates that Griva Vasti is an effective treatment in cervical spondylosis.
ABSTRACT
Indians undergoing socioeconomic and lifestyle transitions will be maximally affected by epidemic of type 2 diabetes (T2D). We conducted a two-stage genome-wide association study of T2D in 12,535 Indians, a less explored but high-risk group. We identified a new type 2 diabetes-associated locus at 2q21, with the lead signal being rs6723108 (odds ratio 1.31; P = 3.32 × 10â»9). Imputation analysis refined the signal to rs998451 (odds ratio 1.56; P = 6.3 × 10⻹²) within TMEM163 that encodes a probable vesicular transporter in nerve terminals. TMEM163 variants also showed association with decreased fasting plasma insulin and homeostatic model assessment of insulin resistance, indicating a plausible effect through impaired insulin secretion. The 2q21 region also harbors RAB3GAP1 and ACMSD; those are involved in neurologic disorders. Forty-nine of 56 previously reported signals showed consistency in direction with similar effect sizes in Indians and previous studies, and 25 of them were also associated (P < 0.05). Known loci and the newly identified 2q21 locus altogether explained 7.65% variance in the risk of T2D in Indians. Our study suggests that common susceptibility variants for T2D are largely the same across populations, but also reveals a population-specific locus and provides further insights into genetic architecture and etiology of T2D.