ABSTRACT
In this work, we prepared silver nanoparticles (AgNPs)-loaded poly(2,5-dimethoxyaniline;PDMA) nanocomposite via a simple chemical oxidative method to develop new effective biocidal and anticancer agents systems. In situ UV-vis and fluorescence spectroscopy has been used to monitor the formation of PDMA/Ag is strongly dependent on the concentration of PDMA in the present system. The FTIR and XRD studies exhibit that the rate of polymer formation and oxidation state of PDMA/Ag nanocomposite, which confirmed the AgNPs is strongly binding with the PDMA matrix. SEM and EDAX analysis revealed the presence of uniform size and the highest percentage of AgNPs (42.1%) in the PDMA/Ag nanocomposite. TEM and size distribution analysis revealed the presence of spherical AgNPs with an average diameter of 25 nm in the PDMA/Ag nanocomposite. In addition, the PDMA polymer with embedded AgNPs exhibited excellent antibacterial performance against Escherichia coli and Staphylococcus aureus. Interestingly, the prepared PDMA/Ag nanocomposite exhibited enhanced bactericidal performance compared with the PDMA polymer, presumably because of the antibacterial properties of the AgNPs substrate. The anticancer effects of combined treatment with PDMA polymer and AgNPs were evaluated using a series of cellular and biochemical assays. The findings from this study revealed that the PDMA/Ag nanocomposite treatment significantly inhibited cell viability and proliferation of the human cervical cancer cell line (HeLa). The PDMA/Ag nanocomposite exhibits better antibacterial and anticancer activities compare to pristine PDMA.
Subject(s)
Antineoplastic Agents , Metal Nanoparticles , Nanocomposites , Anti-Bacterial Agents/pharmacology , Antineoplastic Agents/pharmacology , Humans , Microbial Sensitivity Tests , Polymers , SilverABSTRACT
Seaweed capped zinc oxide nanoparticles (SW-ZnO NPs) were prepared by simple chemical precipitation method of zinc nitrate in the presence of seaweed. The synthesized SW-ZnO NPs were characterised by FT-IR, UV-vis spectroscopy, XRD and BET analysis. These metal oxides were coated on cotton fabric using pad dry cure technique. The SW-ZnO NPs coated cotton fabrics, the surface morphology and chemical composition was analysed by HR-SEM with EDAX. The antibacterial activity of uncoated cotton, seaweed extract and SW-ZnO NPs coated cotton fabrics were investigated against Gram positive (S. aureus and S. pyogenes) and Gram negative (E. coli and K. aerogens). It showed that SW-ZnO NPs coated cotton fabric has higher antibacterial activity than other test samples against both bacteria's. Finally, a UV-protection property of the coated cotton fabric was also tested.