Identification and prioritisation of potential vaccine candidates using subtractive proteomics and designing of a multi-epitope vaccine against Wuchereria bancrofti.

This study employed subtractive proteomics and immunoinformatics to analyze the Wuchereria bancrofti proteome and identify potential therapeutic targets, with a focus on designing a vaccine against the parasite species. A comprehensive bioinformatics analysis of the parasite's proteome identified 51 probable therapeutic targets, among which "Kunitz/bovine pancreatic trypsin inhibitor domain-containing protein" was identified as the most promising vaccine candidate. The candidate protein was used to design a multi-epitope vaccine, incorporating B-cell and T-cell epitopes identified through various tools. The vaccine construct underwent extensive analysis of its antigenic, physical, and chemical features, including the determination of secondary and tertiary structures. Docking and molecular dynamics simulations were performed with HLA alleles, Toll-like receptor 4 (TLR4), and TLR3 to assess its potential to elicit the human immune response. Immune simulation analysis confirmed the predicted vaccine's strong binding affinity with immunoglobulins, indicating its potential efficacy in generating an immune response. However, experimental validation and testing of this multi-epitope vaccine construct would be needed to assess its potential against W. bancrofti and even for a broader range of lymphatic filarial infections given the similarities between W. bancrofti and Brugia.

Larvicidal activity of selected essential oil in synergized combinations against Aedes aegypti.

This study was conducted to attain an alternate plant essential oil (EO) based mosquito larvicide against the dengue vector Aedes aegypti. Here three plants were selected based on their local availability, safety and insecticidal properties. EOs were extracted from Syzygium aromaticum (Myrtaceae) flower buds, fruits of Illicium verum (Schisandraceae) and Trachyspermum ammi (Apiaceae) by hydro-distillation and tested against Ae. aegypti larvae individually and in combinations to find synergistic interactions. Chemical constituent analysis of EOs was done by GC/MS/MS analysis and the main constituents in T. ammi were thymol (93.58%) and terpinen-4-ol (2.16%), in S. aromaticum eugenol 56.32% and caryophyllene 17.91% and in I. verum trans-anethole 53.05% and p-anisaldehyde 12.47%. The EOs from T. ammi, I. verum and S. aromaticum and their top components thymol, trans-anethole and eugenol exhibited larvicidal activity with LC50 values 39.48, 41.30, 66.90, 59.76, 50.19 and 60.89 mgL-1 respectively against Ae. aegypti larvae. The values for the co-toxicity factors for the binary combinations of the EOs were > 20 showing synergistic interactions among the binary mixtures. The respective LC50 values for the 1:1 binary combinations (S. aromaticum + I. verum), (S. aromaticum + T. ammi) and (I. verum + T. ammi) were 49.07, 48.54 and 27.67 mgL-1. ß-cyclodextrin inclusion complex made with I. verum + T. ammi combination showed an LC50 value of 23.93 mgL-1. On the whole the outcome of this study draw attention to the capability of synergistic EO combinations to emerge as a safe and environment friendly effective larvicide to control Aedes mosquitoes.