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Biosensors (Basel) ; 12(2)2022 Jan 26.
Article in English | MEDLINE | ID: mdl-35200330

ABSTRACT

The differentiation of cultivars is carried out by means of morphological descriptors, in addition to molecular markers. In this work, near-infrared spectroscopy (NIR) and chemometric techniques were used to develop classification models for two different commercial sesame cultivars (Sesamum indicum) and 3 different strains. The diffuse reflectance spectra were recorded in the region of 700 to 2500 nm. Based on the application of chemometric techniques: principal component analysis-PCA, hierarchical cluster analysis-HCA, k-nearest neighbor-KNN and the flexible independent modeling of class analogy-SIMCA, from the infrared spectra in the near region, it was possible to perform the genotyping of two sesame cultivars (BRS Seda and BRS Anahí), and to classify these cultivars with 3 different sesame strains, obtaining 100% accurate results. Due to the good results obtained with the implemented models, the potential of the methods for a possible realization of forensic, fast and non-destructive authentication, in intact sesame seeds was evident.


Subject(s)
Sesamum , Spectroscopy, Near-Infrared , Chemometrics , Genotype , Principal Component Analysis , Spectroscopy, Near-Infrared/methods
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