ABSTRACT
Sequence analysis has proved that decamer nucleotides, used as primers of RAPD (random amplified polymorphic DNA), differ with each other greatly in number of annealing sites in the Arabidopsis thaliana genome. It is called the 'primer bias' by the authors. The biased primers produce a highly variable number of amplicons by polymerase chain reaction (PCR). The number of amplicons is proved to correlate with the number of annealing sites. Therefore, a statistical method is proposed for selecting efficient primers based on the primer bias in the genomic sequence. The method was tested by experiment in A. thaliana genome, and the results demonstrate that the method outperforms routine methods and can substantially increase the efficiency of RAPD methodologies. We also proved that the expressed sequence tags (ESTs) show a highly coincident bias pattern with that of the whole genomic sequence, and can therefore be used to assess efficiencies of primers for species whose genomic sequence data are currently unknown.
Subject(s)
Base Sequence , DNA Primers , Random Amplified Polymorphic DNA Technique , Arabidopsis/genetics , Expressed Sequence Tags , Genome, Plant , Polymerase Chain ReactionABSTRACT
To assess the roles of suspected etiological factors in the pathogenic course of primary hepatocellular carcinoma (PHC) in Guangzhou city, a case control study including 100 cases and 100 controls were matched on sex, age, occupation, education degree of was carried out. The results from single factor analysis showed that factors as HBV infection, HCV infection, psychosocial factor, lack of separate kitchen and exhausting hood, habit of eating raw fish meat were related to PHC. Logistic regression analysis showed that HBV, HCV infection, psychic trauma, personality, separate kitchen, habit of eating raw fish meat might be regarded as risk factors for PHC in Guangzhou city. It is noticeable that a significant synergic effect was observed when some factors worked jointly.
