ABSTRACT
Florfenicol (FLO) has been shown to elicit diverse toxic effects in plants, insects, and mammals. Previously, our investigations revealed that FLO induced abnormal cardiac development and early embryonic mortality in chicken embryos. However, the effect of FLO on mitochondrial responses in stem cells remains unclear. In this study, we show that FLO significantly diminishes proliferation viability and obstructs the directed differentiation of P19 stem cells (P19SCs) into cardiomyocytes. Proteomic analysis revealed 148 differentially expressed proteins in response to FLO. Functional analysis has pinpointed FLO interference with biological processes associated with oxidative phosphorylation within the mitochondria. In alignment with the results of proteomic analysis, we confirmed that FLO inhibits the expression of both nuclear DNA-encoded and mitochondrial DNA-encoded subunits of the electron transport chain. Subsequent experiments demonstrated that FLO disrupts mitochondrial dynamics and induces the mitochondrial unfolded protein response to maintain mitochondrial homeostasis. These findings collectively highlight the significance of mitochondrial dynamics and the mitochondrial unfolded protein response to mediate the decreased proliferation viability and directed differentiation potential in P19SCs treated with FLO. In conclusion, this study provides a comprehensive overview of mitochondrial responses to FLO-induced cytotoxicity and enhances our understandings of the molecular mechanisms underlying FLO-induced embryonic toxicity.
ABSTRACT
In the context of predicting pedestrian trajectories for indoor mobile robots, it is crucial to accurately measure the distance between indoor pedestrians and robots. This study aims to address this requirement by extracting pedestrians as regions of interest and mitigating issues related to inaccurate depth camera distance measurements and illumination conditions. To tackle these challenges, we focus on an improved version of the H-GrabCut image segmentation algorithm, which involves four steps for segmenting indoor pedestrians. Firstly, we leverage the YOLO-V5 object recognition algorithm to construct detection nodes. Next, we propose an enhanced BIL-MSRCR algorithm to enhance the edge details of pedestrians. Finally, we optimize the clustering features of the GrabCut algorithm by incorporating two-dimensional entropy, UV component distance, and LBP texture feature values. The experimental results demonstrate that our algorithm achieves a segmentation accuracy of 97.13% in both the INRIA dataset and real-world tests, outperforming alternative methods in terms of sensitivity, missegmentation rate, and intersection-over-union metrics. These experiments confirm the feasibility and practicality of our approach. The aforementioned findings will be utilized in the preliminary processing of indoor mobile robot pedestrian trajectory prediction and enable path planning based on the predicted results.
ABSTRACT
INTRODUCTION: In this article, we utilized Ingenuity® Pathway Analysis (IPA®) bioinformatics analysis software and Metascape® bioinformatics analysis website tools to analyse the possible mechanism of ERH affecting tumourigenesis (proliferation and apoptosis) in bladder cancer (BC) T24 cells. METHODS: The ERH gene was knocked down, and BC T24 cells were divided into ERH normal and knockdown groups. Affymetrix® gene expression microarrays were performed to obtain a differentially expressed gene list (DEGL) between the 2 groups. IPA® data analyses contain five modules: disease and function analysis, upstream analysis, regulator effects analysis, canonical pathway analysis and molecular network analysis. The results of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were analysed by Metascape®. RESULTS: The results of the gene expression profiling chip and the DEGL showed that 344 genes were upregulated and 254 genes were downregulated. The IPA® and Metascape® pathway analyses showed that the ERH gene may affect proliferation and apoptosis by affecting the apoptosis, cell cycle, Toll-like receptor (TLR), NF-κB or TGF-beta signalling pathways. Upstream analysis determined that the ERH gene may regulate TNF and NK-κB in the BC T24 cell lines. The ERH gene may be involved in the "cell death and survival" molecular network in BC T24 cells. ERH may be a regulator of KITLG through TNF. CONCLUSIONS: The ERH gene may affect apoptosis through the TLR, NF-κB, TNF or TGF-beta signalling pathways in BC T24 cells, and may be a regulator of KITLG to ultimately activate the growth of malignant tumours.
Subject(s)
Cell Cycle Proteins/genetics , Transcription Factors/genetics , Urinary Bladder Neoplasms/genetics , Apoptosis/genetics , Carcinogenesis/genetics , Cell Cycle , Cell Cycle Proteins/metabolism , Cell Division , Cell Line, Tumor , Cell Proliferation/genetics , Cell Transformation, Neoplastic/genetics , Computational Biology/methods , Gene Expression/genetics , Gene Expression Profiling/methods , Gene Expression Regulation, Neoplastic/genetics , Gene Knockdown Techniques/methods , Gene Ontology , Humans , Oligonucleotide Array Sequence Analysis , Software , Transcription Factors/metabolism , Urinary Bladder/metabolismABSTRACT
OBJECTIVE: To test the clinical curative effect of Jiutengzhuyu tablets, a patented Chinese vine-derived drug used for promoting blood circulation, on women with oviducal obstruction. METHODS: Patients with tubal infertility were divided randomly into two groups: 58 patients in the treatment group and 57 in a control group. The treated patients took Jiutengzhuyu tablets orally for 25 days. The control group received an intrauterine infusion of 5 mg dexamethasone sodium phosphate, 4000 units of chymotrypsin, 80 000 units of gentamicin sulfate dissolved in 20 mL of normal saline at 1 mL/min. After 3 months of treatment, the curative effect on tubal patency was assessed and a 1-year follow-up visit was used to document any pregnancies. RESULTS: The total effective rate was 78% in the treatment group and 32% in the control group with a statistically significant difference (chi2 = 24.57, P < 0.01). CONCLUSION: Jiutengzhuyu was effective in treating infertility caused by tubal infertility with a Traditional Chinese Medicine diagnosis of blood stasis.
Subject(s)
Blood Circulation/drug effects , Drugs, Chinese Herbal/administration & dosage , Fallopian Tube Diseases/drug therapy , Infertility, Female/drug therapy , Adult , Fallopian Tube Diseases/physiopathology , Female , Humans , Infertility, Female/physiopathology , Young AdultABSTRACT
We report angular and polarization dependent transmission properties of Fano resonance optical filters with transferred silicon nanomembrane on glass substrate. The transmission spectra of the filters can have either weak or strong polarization and angular dependence, depending on properties of individual Fano resonance modal dispersion. Measurement results agree very well with simulations based on a rigorous coupled-wave analysis for the transmission spectra, on planewave expansion wave-vector technique for the dispersion property analysis, and on a three-dimensional finite-difference time-domain technique for the propagating modal study. These results will provide importance guidance for the design of a new class of ultra-compact surface-normal frequency selective components with preferred polarization and angular properties. These components are highly desirable for silicon photonic integration.
