[Analgesic effect of DICLORAL on radiation-induced oral mucositis].

PURPOSE: To assess the effects of DICLORAL's alleviation of pain caused by radiation-induced oral mucositis. METHODS: With compound borax solution as a comparative drugs' the solution of DICLORAL was adopted for patients with radiation-induced oral mucositis due to radiotherapy for nasal NK/T cell lymphoma. All 59 patients were randomly divided into 2 groups: experimental group (using DICLORAL) and control group (using Compound Borax Solution). During the treatment, we observed drugs' ability of alleviate oral pain or pain on swallowing, when it worked and how long it could sustain in controlling pain. Statistical analysis was performed using SPSS 16.0 software package. RESULTS: Drugs' ability of alleviate oral pain or pain on swallowing and the onset time was not significantly different between the 2 groups (P>0.05). Effective duration in experimental group was longer than that in control group (P<0.05). CONCLUSIONS: DICORAL can alleviate and cease pain caused by radiation-induced oral mucositis effectively.

Construction of the seed-coat cDNA microarray and screening of differentially expressed genes in barley.

Some barley mutants can synthesize neither anthocyanins nor proanthocyanidins in the seed coat, which is related to several genes in locus Ant13, but the exact model of action remains unknown. We used the cDNA microarray technology with barley transcription-deficient mutant (ant13-152) that does not synthesize proanthocyanidins as the tester, and its wild type genotype (Triumph) as the driver, to study this question. Six-thousand and forty-eight clones from the wild type Morex testa+pericarp cDNA library were amplified using PCR, and the DNA fragments were spotted on commercial amino-modified glass slide as microarray. The mRNAs from the developing seed coat (8-15 days) of both the mutant and the wild-type barley plants were isolated, and labeled respectively with Cy3-dUTP and Cy5-dUTP when reversely transcribed to cDNAs. The labeled cDNAs were used as probes, mixed at the same molar concentration, and hybridized with the DNA fragments on the slide. Seventy clones exhibiting marked differential expression (ratio>4) were identified from the microarray. All the 25 cDNA clones that showed an over-expression in wild type in comparison to the mutant ant13-152 were sequenced. It was found that most of these overexpressing clones were transcription/translation and hordein-associated genes. These results have laid a solid material basis for further elucidation of the metabolic pathway in proanthocyanidin synthesis in barley and likely other plants.