ABSTRACT
Stromal cells, such as myofibroblasts and fibroblasts, represent a significant fraction of MHC class II-positive cells in the normal human colonic lamina propria, suggesting they may play an important role in CD4(+) T cell regulation in a tolerogenic environment. The aim of this study was to examine whether human colonic myofibroblasts (CMFs) phenotypically and functionally resemble conventional antigen-presenting cells (APCs). Our results support the hypothesis that intestinal myofibroblasts are a novel, nonprofessional APC phenotype important in modulating mucosal T cell responses. Given their strategic location, we propose that intestinal myofibroblasts play a critical role in mediating tolerance to luminal antigens.
Subject(s)
Fibroblasts/immunology , HLA-D Antigens/immunology , Immune Tolerance , Immunity, Mucosal , Intestines/immunology , Muscle, Smooth/immunology , Cells, Cultured , Dendritic Cells/immunology , Humans , Immunity, Mucosal/immunologyABSTRACT
A model is presented describing the thermoelastic photoacoustic interaction in a layered medium within a transparent fluid, where a polymer transducer is used for the detection of ultrasonic pulses. By taking the optical absorption coefficient and finite layer thickness into account, the amplitude and shape of photoacoustic transients are calculated for both forward and backward travelling directions. Additionally, photoacoustic transient interaction with the PVDF transducer has been characterised using a discrete-time algorithm for the transducer response. Good agreement with experimental waveforms are demonstrated, so that this may form the basis of system characterisation when miniature laser-ultrasound probes are used in various applications.
