ABSTRACT
In this study, we constructed a high effective fusion expression-vector in E. coli. This vector, pTO-T7, was characterized as: (1) an enhancer from tobacco mosaic virus (TMV), omega sequence, was ligated in front of a T7 promoter in the regulatory sequence; (2) the multi-cloning sites include eight restriction enzyme sites. It can facilitate fusion or nonfusion expression; (3) the N terminal of a fusion protein starts with the first 12 amino acids of T7 gene 10, and the C terminal is the hexahistidine tag; (4) kanmycin resistance gene was used as a selective marker. EGFP gene was inserted into pTO-T7 vector as a reporter gene. Expression data showed that fused-EGFP accounted to more than 50% of the total E. coli protein, and more than 90% of which was soluble. The fluorescence characters of fused-EGFP were also studied. The expression yield of target gene from plasmid pTO-T7 compared with that from pT-T7 without omega sequence suggested that omega sequence in pTO-T7 can improve the expression of target gene significantly.
Subject(s)
Enhancer Elements, Genetic , Escherichia coli/genetics , Genetic Vectors , Base Sequence , Green Fluorescent Proteins , Luminescent Proteins/genetics , Molecular Sequence DataABSTRACT
Based on etiological analysis of 246 cases of oculomotor palsy, the causes of the disease were firstly aneurysm of the skull base and intracranial inflammation, followed by intracranial tumor and skull injury. Aneurysm of the skull base with oculomotor palsy often manifested pupil dilation. Syndromes associated with oculomotor palsy were discussed.
Subject(s)
Oculomotor Nerve Diseases/etiology , Adolescent , Adult , Aged , Arachnoiditis/complications , Child , Child, Preschool , Female , Humans , Intracranial Aneurysm/complications , Male , Meningeal Neoplasms/complications , Meningioma/complications , Meningitis/complications , Middle AgedABSTRACT
With gas chromatography, we established a method of determining plasma very long-chain fatty acids (VLCFA). Eight cases with adrenoleukodystrophy (ALD) were confirmed by using plasma VLCFA assay as the diagnostic method. The phenotypes of the 8 patients are as follows: 1. Childhood ALD; 2. Adolescent ALD; 3. Adult cerebral ALD: 4. Adrenomyeloneuropathy; 5. Addison's disease without neurologic involvement; 6. Asymptomatic ALD. We do not agree with Moser's classification of ALD as presymptomatic and asymptomatic, but would suggest to have a subclinical type of ALD.
Subject(s)
Adrenoleukodystrophy/classification , Fatty Acids/blood , Adolescent , Adrenoleukodystrophy/diagnosis , Adult , Child , Child, Preschool , Chromatography, Gas , Female , Humans , MaleABSTRACT
Several erythrocyte biophysical properties (erythrocyte deformability, erythrocyte aggregation and erythrocyte electrophoretic mobility) were investigated in patients with cerebral infarction and in individuals with risk factors of stroke (RFS population; RFSP). Blood viscosity, plasma viscosity, hematocrit, plasma fibrinogen level and yield shear stress (YSS) were also tested. In comparison with the results in a control group, erythrocyte deformability (erythrocyte length under a certain constant shear stress and erythrocyte filterability) was less in both the patient group and the group of RFSP and there is an accompanying increase of erythrocyte aggregation. Blood viscosity and fibrinogen level were higher in the patient groups. There was no correlation between erythrocyte deformability and other hemorheological parameters. Our results suggest that significant alteration of erythrocyte biophysical properties may be implicated in the pathogenesis of ischemic stroke. These abnormalities are associated with some of the risk factors of stroke such as hypertension, atherosclerosis and cardiovascular disorders.
