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1.
Mol Neurobiol ; 54(5): 3137-3148, 2017 07.
Article in English | MEDLINE | ID: mdl-27052954

ABSTRACT

The nucleoside guanosine (GUO) increases glutamate uptake by astrocytes and acts as antioxidant, thereby providing neuroprotection against glutamatergic excitotoxicity, as we have recently demonstrated in an animal model of chronic hepatic encephalopathy. Here, we investigated the neuroprotective effect of GUO in an acute ammonia intoxication model. Adult male Wistar rats received an intraperitoneal (i.p.) injection of vehicle or GUO 60 mg/kg, followed 20 min later by an i.p. injection of vehicle or 550 mg/kg of ammonium acetate. Afterwards, animals were observed for 45 min, being evaluated as normal, coma (i.e., absence of corneal reflex), or death status. In a second cohort of rats, video-electroencephalogram (EEG) recordings were performed. In a third cohort of rats, the following were measured: (i) plasma levels of glucose, transaminases, and urea; (ii) cerebrospinal fluid (CSF) levels of ammonia, glutamine, glutamate, and alanine; (iii) glutamate uptake in brain slices; and (iv) brain redox status and glutamine synthetase activity in cerebral cortex. GUO drastically reduced the lethality rate and the duration of coma. Animals treated with GUO had improved EEG traces, decreased CSF levels of glutamate and alanine, lowered oxidative stress in the cerebral cortex, and increased glutamate uptake by astrocytes in brain slices compared with animals that received vehicle prior to ammonium acetate administration. This study provides new evidence on mechanisms of guanine-derived purines in their potential modulation of glutamatergic system, contributing to GUO neuroprotective effects in a rodent model of by acute ammonia intoxication.


Subject(s)
Ammonia/toxicity , Guanosine/pharmacology , Neuroprotective Agents/pharmacology , Animals , Biomarkers/blood , Biomarkers/cerebrospinal fluid , Brain/metabolism , Coma/blood , Coma/cerebrospinal fluid , Coma/chemically induced , Coma/drug therapy , Disease Models, Animal , Electroencephalography , Guanosine/therapeutic use , Male , Neuroprotective Agents/therapeutic use , Oxidation-Reduction , Oxidative Stress/drug effects , Rats, Wistar
2.
Metab Brain Dis ; 29(3): 645-54, 2014 Sep.
Article in English | MEDLINE | ID: mdl-24788896

ABSTRACT

It is well known that glutamatergic excitotoxicity and oxidative stress are implicated in the pathogenesis of hepatic encephalopathy (HE). The nucleoside guanosine exerts neuroprotective effects through the antagonism against glutamate neurotoxicity and antioxidant properties. In this study, we evaluated the neuroprotective effect of guanosine in an animal model of chronic HE. Rats underwent bile duct ligation (BDL) and 2 weeks later they were treated with i.p. injection of guanosine 7.5 mg/kg once a day for 1-week. We evaluated the effects of guanosine in HE studying several aspects: a) animal behavior using open field and Y-maze tasks; b) brain rhythm changes in electroencephalogram (EEG) recordings; c) purines and glutamate levels in the cerebral spinal fluid (CSF); and d) oxidative stress parameters in the brain. BDL rats presented increased levels of glutamate, purines and metabolites in the CSF, as well as increased oxidative damage. Guanosine was able not only to prevent these effects but also to attenuate the behavioral and EEG impairment induced by BDL. Our study shows the neuroprotective effects of systemic administration of guanosine in a rat model of HE and highlights the involvement of purinergic system in the physiopathology of this disease.


Subject(s)
Behavior, Animal/drug effects , Brain/drug effects , Guanosine/therapeutic use , Hepatic Encephalopathy/drug therapy , Neuroprotective Agents/therapeutic use , Oxidative Stress/drug effects , Animals , Brain/metabolism , Electroencephalography , Guanosine/pharmacology , Hepatic Encephalopathy/metabolism , Male , Neuroprotective Agents/pharmacology , Oxidation-Reduction , Rats , Rats, Wistar , Reactive Oxygen Species/metabolism , Thiobarbituric Acid Reactive Substances/metabolism
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