ABSTRACT
The effects of cis-diamminedichloroplatinum (II) (cisplatin), a drug used in cancer chemotherapy, on the oxidative metabolism, endocytosis, chemotaxis and exocytosis of guinea-pig polymorphonuclear leukocytes were studied. All these functions were negatively influenced, but the same effect (50% inhibition) was observed at different drug concentrations (3 X 10(-5) M for chemotaxis, 10(-4) M for O2 consumption by FMLP and beta-glucuronidase release, 10(-3) M for O2 consumption by PMA and for zymosan engulfment). The effects of the drug can be explained by its ability to bind to membrane proteins, essentially to -SH groups.
Subject(s)
Cisplatin/pharmacology , Neutrophils/drug effects , Phagocytosis/drug effects , Animals , Chemotaxis/drug effects , Cytochalasin B/pharmacology , Dose-Response Relationship, Drug , Endocytosis/drug effects , Exocytosis/drug effects , Glucuronidase/metabolism , Guinea Pigs , In Vitro Techniques , Neutrophils/physiology , Oxygen Consumption/drug effects , Peritoneum/cytologyABSTRACT
The authors have investigated the chemotactic ability of C. albicans toward macrophages and polymorphonuclears. Experiments, carried out by modified Boyden chamber, lead to isolate a chemotactic agent, considered either a moderate cytotaxin, or an excellent cytotaxigen.
Subject(s)
Candidiasis/immunology , Immunity, Cellular , Animals , Cell Movement , Chemotaxis, Leukocyte , Humans , In Vitro Techniques , Macrophages/immunology , Monocytes/immunology , Neutrophils/immunology , RatsABSTRACT
Macrophage chemotactic activity was studied as a marker of the anti-inflammatory effect accompanying tumour growth. An inhibiting effect of the tumour on the macrophage mobility was observed by comparing the macrophages collected from normal rats and those from animals bearing the IRE reticulosarcoma. To explain these results, it is postulated that a MIF, or a similar inhibiting factor is released by the tumour. Both the the nature and function of such a factor(s) remain to be clarified.
Subject(s)
Chemotaxis , Lymphoma, Non-Hodgkin/immunology , Macrophages/immunology , Animals , Lymphoma, Non-Hodgkin/metabolism , Macrophage Migration-Inhibitory Factors/metabolism , Neoplasm Transplantation , Rats , Sarcoma, Experimental/immunology , Sarcoma, Experimental/metabolism , Transplantation, HomologousABSTRACT
Leukocyte inhibiting factor (LIF) release from lymphocytes cultures stimulated by bacterial lipopolysaccharides (LPS) has been investigated. Human lymphocytes cultured in the presence of E, coli or S. enteritidis LPS were able to release in the supernatants a substance with the following properties: a) same elution pattern of Bovine Serum Albumin (MW 69000) when filtered on Sephadex G-100; b) it migrates on disc electrophoresis a an albumin; c) not dialyzable; d) not cytotoxic; e) inhibition of polymorphonuclear cells migration in agarose plates; f) heat stability at 56 degrees C for 30'. This substance could be likely identified as LIF.
