Removal and monitoring acetaminophen-contaminated hospital wastewater by vertical flow constructed wetland and peroxidase enzymes.

Hospital wastewater contains acetaminophen (ACT) and nutrient, which need adequate removal and monitoring to prevent impact to environment and community. This study developed a pilot scale vertical flow constructed wetland (CW) to (1) remove high-dose ACT and pollutants in hospital wastewater and (2) identify the correlation of peroxidase enzyme extruded by Scirpus validus and pollutants removal efficiency. By that correlation, a low-cost method to monitor pollutants removal was drawn. Plants, such as Scirpus validus, generated peroxidase enzymes to alleviate pollutants' stress. Results showed that the CW removed 3.5 to 6 logs of initial concentration 10 mg ACT/L to a recommended level for drinking water. The CW eliminated COD, TKN and TP efficiently, meeting the wastewater discharged standards of Thailand and Vietnam. By various multivariable regression models, concentrations of ACT in CW effluent and enzymes in S. validus exhibited a significant correlation (p < 0.01, R2 = 68.3%). These findings suggested that (i) vertical flow CW could remove high-dose ACT and nutrient and (ii) peroxidase enzymes generated in S. validus, such as soluble and covalent ones, could track ACT removal efficiency. This would help to reduce facilities and analytical cost of micro-pollutants.

Hydraulic behaviour of vertical-flow constructed wetland under different operating conditions.

Five vertical-flow constructed wetland (VFCW) units planted with cattail (Typha augustifolia) were used to study the effects of feeding (continuous and batch), hydraulic loading rates (HLR) and drainage patterns (free drainage and percolate impounding) on hydraulic behaviour. The tracer studies were divided into two parts: (i) continuous feeding at an HLR of 0.005, 0.025, 0.05, 0.075, 0.1 and 0.3 m3 m(-2) d(-1) operating at different drainage patterns (i.e. free drainage and percolate impounding) and (ii) batch feeding at different water levels of percolate impounding (20, 30 and 40 cm). The results revealed that although the hydraulic behaviour of VFCW systems was strongly dependent on the operating pattern (feeding and drainage), it was not significantly affected by the hydraulic loading rate. The results of continuous feeding study concluded that (a) percolate impounding achieved an increase in HRT that was 1.6 times the HRT with free drainage, (b) the dispersion in both drainage patterns were moderate, and (c) the results from the tank-in-series (TIS) model correlated more closely with the data observed than with the dispersion plug flow (DPF) model. For batch feeding and percolate impounding, a uniform flow distribution of the tracer occurred in the water column after 2.1, 3.1 and 4.4 days for 20, 30 and 40 cm, respectively.

Influence of sand layer depth and percolate impounding regime on nitrogen transformation in vertical-flow constructed wetlands treating faecal sludge.

Four laboratory-scale units of vertical-flow constructed wetlands (VFCW) were fed once a week with faecal sludge (FS) at a constant solids loading rate (SLR) of 250 kg TS/(m2.year) (equivalent to 260-300 gN/(m2.week)) for a period of 12 weeks to study: i) the nitrification and denitrification potential of the sand layer of VFCWs and ii) the effect of percolate impounding regime (permanent or batch-impounding) on nitrogen transformation. The TN content of raw FS was characterised by 65% org-N, 34% NH4-N and 1% NOx-N. After FS application and a six-day impounding period, 8-13% TN were recovered in the percolate exhibiting the following composition: 70-80% NH4-N, 25-30% org-N and <1% NOx-N. A large fraction of the influent organic N (55%) was filtered in the bed and 24-29% of initial NH4-N were lost due to nitrification and volatilisation. In permanent impounding systems, 8-11% TN were recovered in the percolate versus 13% in batch-operated beds. N loss was increased with sand layer depth (20-40 cm) under permanent impounding regimes.