Aging and lymphocyte subsets in the spleen and peripheral blood of the Sprague-Dawley rat.

This study was undertaken to determine the effects of aging on lymphocyte subsets in the peripheral blood and spleens of Sprague-Dawley rats. Rats aged 3,13 and 26 months were used in the study. Analyses of dual labeled lymphocytes from the 26 month animals show decreases in the numbers of lymphocytes due to decreased cellularity (spleen) or reduced lymphocyte percentages within the total white blood cell population (peripheral blood). In the spleens and blood of the oldest rats, there were reduced numbers of Total T, T helper/amplifier (Th/a), virgin Th and natural killer (NK) cells. Other changes were observed in the spleen but not peripheral blood. The numbers of T cytotoxic/suppressor cells (Tc/s) B cells, "autoimmune" B cells and NK cells were reduced in the spleen but remained within normal limits in peripheral blood. The data show aging exerts different effects on the peripheral blood and splenic compartments of the immune system. These differences may have teleological significance in relation to immune responses to xenobiotics and neoplastic cells.

The effect of continuous corticosterone administration on lymphocyte subpopulations in the peripheral blood of the Fischer 344 rat as determined by two color flow cytometric analyses.

This study was undertaken to determine the effects of continuous corticosterone administration on lymphocyte subsets in the peripheral blood of Fischer 344 rats. Pellets which released corticosterone over a 21 day period (0.07 mg/day, 0.48 mg/day and 4.8 mg/day) were implanted subcutaneously in male rats. Control rats received pellets containing only the excipient carrier. Rats in the test and control groups were sacrificed at 7, 14 and 21 days. Lymphocyte subsets were enumerated by dual color flow cytometry and the data expressed in absolute numbers/mm3. Effects were observed only in the animals treated with the highest dose which was 70,000 times the normal plasma level. The spleen, thymus and lymph nodes were examined for histopathological changes. At the seven day sacrifice there was a statistically significant decrease in total white blood cells and selective decrements in lymphocytes with reductions in the absolute numbers of the T helper/amplifier, T cytotoxic/suppressor and B cells. Only numbers of natural killer cells were within normal limits. Histopathological data from animals treated with the high dose corticosterone for seven days demonstrated decreased thymic weights and a loss of thymic lymphocytes. At 14 and 21 days, the numbers of lymphocytes returned to the normal range, but the numbers of total T cells remained decreased. Also, thymic weights were reduced but not histological abnormalities were observed in the thymus. The data suggest that corticosterone induced a persistent decrease in total T cells, but only a transient effect on total lymphocytes.

The effect of Lasso herbicide on human immune function as measured by in vitro assays.

Using in vitro assays, this study was undertaken to determine whether the components of Lasso herbicide formulation had an effect on the human immune system. Mononuclear cells from human peripheral blood were exposed to analytical alachlor, alachlor conjugated to human serum albumin or Lasso formulation over a concentration range from .01 microM-1.0 microM. The effects of the test materials on the following immunological functions were determined: lymphocyte proliferation induced by mitogen or antigen; antibody synthesis of IgG and IgM isotypes in pokeweed stimulated mononuclear cell cultures; cytotoxic T cell proliferation; lysis of target cells by natural killer cells and lymphokine activated killer cells. The data demonstrated that the test compounds had no significant, dose related effect on the function of immunocompetent cells. Hence, the data suggest that the components of the Lasso formulation have no effect on the human immune system.