Latitudinal variation in survival and immature development of Ceratitis capitata populations reared in two key overwintering hosts.

Ceratitis capitata, a major agricultural pest, is currently expanding its geographic distribution to northern, temperate areas of Europe. Its seasonal biology and invasion success depend on temperature, humidity and host availability. In coastal warmer Mediterranean regions and cooler temperature areas, bitter oranges and apples serve as overwintering hosts during the larval stage. We assessed the overwintering capacity of C. capitata populations obtained from different areas of the northern hemisphere by studying the survival and development rates of immature stages in both fruits under laboratory conditions. Eggs from each population were artificially inserted in the flesh of the two hosts and kept at 15, 20, or 25 °C until pupation and adult emergence. Climatic analysis of the area of the population origin showed combined effects of latitude, host and macroclimatic variables on immature survival and development rates. Egg to adult survival rates and developmental duration were longer in apples than in bitter oranges. For populations originated from southern-warmer areas, egg to adult developmental duration was prolonged and adult emergence reduced at 15 °C compared to those populations obtained from northern regions. Our findings reveal varying plastic responses of medfly populations to different overwintering hosts and temperatures highlighting the differential overwintering potential as larvae within fruits. This study contributes towards better understanding the medfly invasion dynamics in temperate areas of Northern Europe and other parts of the globe with similar climates.

Differential Cold Tolerance on Immature Stages of Geographically Divergent Ceratitis capitata Populations.

Cold tolerance of adult medflies has been extensively studied but the effect of subfreezing temperatures on the immature stages remains poorly investigated, especially as far as different populations are regarded. In this study, we estimated the acute cold stress response of three geographically divergent Mediterranean fruit fly populations originating from Greece (Crete, Volos) and Croatia (Dubrovnik) by exposing immature stages (eggs, larvae, pupae) to subfreezing temperatures. We first determined the LT50 for each immature stage following one hour of exposure to different temperatures. Then eggs, larvae and pupae of the different populations were exposed to their respective LT50 for one hour (LT50 = -11 °C, LT50 = -4.4 °C, LT50 = -5 °C for eggs, larvae and pupae, respectively). Our results demonstrate that populations responded differently depending on their developmental stage. The population of Dubrovnik was the most cold-susceptible at the egg stage, whereas in that of Crete it was at the larval and pupal stage. The population of Volos was the most cold-tolerant at all developmental stages. The egg stage was the most cold-tolerant, followed by pupae and finally the 3rd instar wandering larvae. This study contributes towards understanding the cold stress response of this serious pest and provides data for important parameters that determine its successful establishment to unfavorable environments with an emphasis on range expansion to the northern, more temperate regions of Europe.

Controlling the wettability of stainless steel from highly-hydrophilic to super-hydrophobic by femtosecond laser-induced ripples and nanospikes.

Results on the manipulation of the wetting properties of stainless steel alloy surface by ultrashort pulse laser texturing are presented. The wide range of water droplet contact angles from highly-hydrophilic to super-hydrophobic was achieved by generation of laser-induced periodic surface structures (LIPSS) and nanospikes. In particular, the wetting state was controlled by accumulated laser fluence, which determines the carbon/oxygen content and nano-texture type of the surface after laser treatment. A super-hydrophobic water-repelling surface was generated. The simple, single-step laser processing technology was demonstrated as a promising tool for the large-scale industrial production of self-cleaning stainless steel.

Biomimetic Omnidirectional Antireflective Glass via Direct Ultrafast Laser Nanostructuring.

Here, a single-step, biomimetic approach for the realization of omnidirectional transparent antireflective glass is reported. In particular, it is shown that circularly polarized ultrashort laser pulses produce self-organized nanopillar structures on fused silica (SiO2 ). The laser-induced nanostructures are selectively textured on the glass surface in order to mimic the spatial randomness, pillar-like morphology, as well as the remarkable antireflection properties found on the wings of the glasswing butterfly, Greta oto, and various Cicada species. The artificial structures exhibit impressive antireflective properties, both in the visible and infrared frequency ranges, which are remarkably stable over time. Accordingly, the laser-processed glass surfaces show reflectivity smaller than 1% for various angles of incidence in the visible spectrum for s-p linearly polarized configurations. However, in the near-infrared spectrum, the laser-textured glass shows higher transmittance compared to the pristine. It is envisaged that the current results will revolutionize the technology of antireflective transparent surfaces and impact numerous applications from glass displays to optoelectronic devices.

Weed mapping in cotton using ground-based sensors and GIS.

Site-specific weed management presupposes the careful monitoring and mapping of weed infestation areas. Cut-edge sensor technologies coupled with geographical information systems (GIS) provide the means for reliable decision-making concerning weed management even in sub-field level. In present research, two different spectral sensing systems were engaged in order to digitally map weed patches as grown in four different cotton fields in Central Greece. The systems used were a set of two Crop Circle multispectral sensors ACS-430 and a digital camera Nikon D300S. The spaces between cotton rows were scanned and photographed with the two systems accordingly. Raw recorded data were stored and analyzed in GIS environment producing spatially interpolated maps of red-edge normalized difference vegetation index (NDVI) and weed cover percentage values. Both mapping approaches were satisfactorily related to weed distribution as occurred in the fields; however, the photographic method tended to underestimate weed populations. Correlation of red-edge NDVI and weed cover values, at the points where photographs were taken, as revealed by Pearson's correlation coefficient was high (r > 0.83) and statistically significant at the 0.01 level. A first-degree linear equation adequately modeled (R2 > 0.7) the between value pair relations, strengthening the validity of the two methodologies in spatially monitoring weed patches. The methodologies and the technologies used in the study can be used for yearly mapping weed flora in cotton cultivation and potentially constitute a means of rationalizing herbicide application in terms of doses and spatio-temporal decision-making.

Spatio-temporal monitoring of cotton cultivation using ground-based and airborne multispectral sensors in GIS environment.

Multispectral sensor capability of capturing reflectance data at several spectral channels, together with the inherent reflectance responses of various soils and especially plant surfaces, has gained major interest in crop production. In present study, two multispectral sensing systems, a ground-based and an aerial-based, were applied for the multispatial and temporal monitoring of two cotton fields in central Greece. The ground-based system was Crop Circle ACS-430, while the aerial consisted of a consumer-level quadcopter (Phantom 2) and a modified Hero3+ Black digital camera. The purpose of the research was to monitor crop growth with the two systems and investigate possible interrelations between the derived well-known normalized difference vegetation index (NDVI). Five data collection campaigns were conducted during the cultivation period and concerned scanning soil and plants with the ground-based sensor and taking aerial photographs of the fields with the unmanned aerial system. According to the results, both systems successfully monitored cotton growth stages in terms of space and time. The mean values of NDVI changes through time as retrieved by the ground-based system were satisfactorily modelled by a second-order polynomial equation (R 2 0.96 in Field 1 and 0.99 in Field 2). Further, they were highly correlated (r 0.90 in Field 1 and 0.74 in Field 2) with the according values calculated via the aerial-based system. The unmanned aerial system (UAS) can potentially substitute crop scouting as it concerns a time-effective, non-destructive and reliable way of soil and plant monitoring.

Revisiting Greek Propolis: Chromatographic Analysis and Antioxidant Activity Study.

Propolis is a bee product that has been extensively used in alternative medicine and recently has gained interest on a global scale as an essential ingredient of healthy foods and cosmetics. Propolis is also considered to improve human health and to prevent diseases such as inflammation, heart disease, diabetes and even cancer. However, the claimed effects are anticipated to be correlated to its chemical composition. Since propolis is a natural product, its composition is consequently expected to be variable depending on the local flora alignment. In this work, we present the development of a novel HPLC-PDA-ESI/MS targeted method, used to identify and quantify 59 phenolic compounds in Greek propolis hydroalcoholic extracts. Amongst them, nine phenolic compounds are herein reported for the first time in Greek propolis. Alongside GC-MS complementary analysis was employed, unveiling eight additional newly reported compounds. The antioxidant activity study of the propolis samples verified the potential of these extracts to effectively scavenge radicals, with the extract of Imathia region exhibiting comparable antioxidant activity to that of quercetin.

Organic pollution and its effects in the marine mussel Mytilus galloprovincialis in Eastern Mediterranean coasts.

Persistent chemicals and emerging pollutants are continuously detected in marine waters and biota. Out of these, polycyclic aromatic hydrocarbons (PAHs) and organochlorine pesticides (OCs) are significant contaminants with decades of presence in the marine environment. The Mediterranean Sea is an ecosystem directly affected by a variety of anthropogenic activities including industry, municipal, touristic, commercial and agricultural. The Mediterranean mussel (Mytilus galloprovincialis) is a filter feeder, which presents wide distribution. In this regard, the specific organism was used as a biological indicator for the monitoring and evaluation of pollution in the studied areas with focus on the mentioned chemical groups. Pristine Turkish sites with minimum effect from anthropogenic activities, in contrast with Greek sites which were subjected to heavy industrial and shipping activity, were selected. A gas chromatographic tandem mass spectrometric method (GC-MS/MS) was developed and validated to monitor 34 compounds (16 EPA priority PAHs and 18 OCs). Analyses of mussel samples in 2011 from sites with the limited anthropogenic pollution shores have shown the occurrence of 11 pollutants (6 PAHs, 5 OCs), while in the samples from sites with intensive activity and expected pollution, 12 PAHs and 6 OCs were detected. Biochemical and biological responses studied only in mussels samples from the sites with the highest contamination showed a situation that was under strong seasonal influence. The intensity of the response was also influenced by deployment duration. Noteworthy correlations were detected among biochemical/biological effects and between mussel body burden and these effects. Continuous monitoring of priority pollutants of East Mediterranean Sea is vital both for ecological and human risk assessment purposes.