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1.
Sensors (Basel) ; 21(11)2021 May 22.
Article in English | MEDLINE | ID: mdl-34067441

ABSTRACT

The development and application of a low-cost instrumentation system for seismic hazard assessment in urban areas are described in the present study. The system comprises a number of autonomous triaxial accelerographs, designed and manufactured in house and together with dedicated software for device configuration, data collection and further postprocessing. The main objective is to produce a detailed view of strong motion variability in urban areas, for at least light intensity strong motion events. The overall cost of the developed devices is at least ten times lower than the respective commercial units, hence their deployment as an ultra-dense network over the area of interest can be significantly cost-effective. This approach is considered an efficient complement to traditional microzonation procedures, which are typically based on relatively few actual recordings and the application of theoretical methodologies to assess the strong motion distribution. The manufactured devices adopt micro-electro-mechanical (MEMS) digital sensor technology for recording acceleration, whereas the accompanying software suite provides various configuration options, quick browsing, analyzing and exporting of the recorded events, as well as GIS type functionality for seamlessly producing explicit seismic hazard maps of the considered area. The evaluation of system performance was based on shaking table and real field comparisons against high accuracy commercial accelerographs. The study concludes with a real application of the proposed system in the form of an ultra-dense network installed at the city of Lefkada, an earthquake prone urban area in Greece, and the following compilation of explicit shakemaps.

2.
Neuropharmacology ; 117: 408-421, 2017 05 01.
Article in English | MEDLINE | ID: mdl-28219718

ABSTRACT

The Regulator of G protein Signalling 4 (RGS4) is a multitask protein that interacts with and negatively modulates opioid receptor signalling. Previously, we showed that the δ-opioid receptor (δ-OR) forms a multiprotein signalling complex consisting of Gi/Go proteins and the Signal Transducer and Activator of Transcription 5B (STAT5B) that leads to neuronal differentiation and neurite outgrowth upon δ-ΟR activation. Here, we investigated whether RGS4 could participate in signalling pathways to regulate neurotropic events. We demonstrate that RGS4 interacts directly with STAT5B independently of δ-ΟR presence both in vitro and in living cells. This interaction involves the N-terminal portion of RGS4 and the DNA-binding SH3 domain of STAT5B. Expression of RGS4 in HEK293 cells expressing δ-OR and/or erythropoietin receptor results in inhibition of [D-Ser2, Leu5, Thr6]-enkephalin (DSLET)-and erythropoietin-dependent STAT5B phosphorylation and subsequent transcriptional activation. DSLET-dependent neurite outgrowth of neuroblastoma cells is also blocked by RGS4 expression, whereas primary cortical cultures of RGS4 knockout mice (RGS4-/-) exhibit enhanced neuronal sprouting after δ-OR activation. Additional studies in adult brain extracts from RGS4-/- mice revealed increased levels of p-STAT5B. Finally, neuronal progenitor cultures from RGS4-/- mice exhibit enhanced proliferation with concomitant increases in the mRNA levels of the anti-apoptotic STAT5B target genes bcl2 and bcl-xl. These observations suggest that RGS4 is implicated in opioid dependent neuronal differentiation and neurite outgrowth via a "non-canonical" signaling pathway regulating STAT5B-directed responses.


Subject(s)
Neurogenesis/physiology , Neuronal Outgrowth/physiology , Neurons/metabolism , RGS Proteins/metabolism , STAT5 Transcription Factor/metabolism , Animals , Cell Line, Tumor , Cell Survival/physiology , Cerebral Cortex/metabolism , HEK293 Cells , Humans , Mice, Inbred C57BL , Mice, Knockout , Neural Stem Cells/metabolism , Phosphorylation/physiology , Proto-Oncogene Proteins c-bcl-2/metabolism , RGS Proteins/genetics , RNA, Messenger/metabolism , Rats , Receptors, Erythropoietin/metabolism , Receptors, Opioid, delta/metabolism , bcl-X Protein/metabolism
3.
Cell Oncol (Dordr) ; 38(2): 155-64, 2015 Apr.
Article in English | MEDLINE | ID: mdl-25539992

ABSTRACT

PURPOSE: Tamoxifen is a major treatment modality for estrogen receptor positive breast cancer, but the occurrence of resistance remains a problem. Recently, obesity-related leptin has been found to interfere with tamoxifen in breast cancer MCF-7 cells. In the present study we investigated the effect of leptin on three tamoxifen-treated breast cancer cell types (i.e., MDA-MB-231, MCF-7 and MCF-7/HER2). METHODS: The effect of tamoxifen/leptin treatment was evaluated using a MTT cell viability assay. mRNA expression was assessed by real time PCR and protein expression by Western blotting. WWOX, Survivin and BCL2 gene promoter activities were evaluated by chromatin immunoprecipitation. RESULTS: Cell viability assays revealed that estrogen receptor negative MDA-MB-231 cells were resistant, that estrogen receptor positive MCF-7 cells were sensitive and that MCF-7/HER2 cells were relatively resistant to tamoxifen, while leptin co-administration 'rescued' MCF-7 and, especially, MCF-7/HER2 cells from the anti-proliferative effect of tamoxifen. The cell lines also exhibited a different phosphorylation status of STAT3, a transcription factor that is activated by the obesity related leptin receptor b (Ob-Rb). Most importantly, chromatin immunoprecipitation assays revealed differential STAT3 binding to the anti-apoptotic BCL2 and pro-apoptotic WWOX gene promoters in MCF-7 and MCF-7/HER2 cells, leading to concomitant modifications of its mRNA/protein expression levels, thus providing a selective advantage to HER2 over-expressing MCF-7/HER2 cells after treatment with tamoxifen and tamoxifen plus leptin. CONCLUSIONS: Our study provides novel evidence indicating that synergy between the leptin/Ob-Rb/STAT3 signalling pathway and the HER2 receptor protects tamoxifen-treated HER2 over-expressing cells from the inhibitory effect of tamoxifen through differential regulation of apoptosis-related genes.


Subject(s)
Apoptosis/drug effects , Breast Neoplasms/metabolism , Drug Resistance, Neoplasm/drug effects , Leptin/metabolism , Receptor, ErbB-2/metabolism , Apoptosis/physiology , Blotting, Western , Cell Line, Tumor , Chromatin Immunoprecipitation , Drug Resistance, Neoplasm/physiology , Female , Gene Expression Regulation, Neoplastic/drug effects , Humans , Leptin/pharmacology , Real-Time Polymerase Chain Reaction , STAT3 Transcription Factor/metabolism , Selective Estrogen Receptor Modulators/pharmacology , Signal Transduction/drug effects , Signal Transduction/physiology , Tamoxifen/pharmacology
4.
Anticancer Res ; 33(5): 1977-81, 2013 May.
Article in English | MEDLINE | ID: mdl-23645746

ABSTRACT

BACKGROUND: Cell adhesion proteins that connect each cell to neighboring cells and the extracellular matrix play a fundamental role in metastasis. Mitogen-inducible gene-2 (MIG2), is a cell-matrix adhesion protein, which through migfilin, interacts with filamin-A, being linked to actin cytoskeleton. AIM: Recent studies have implicated both MIG2 and migfilin in cancer, but little is known regarding their expression in breast cancer. In this study, we investigated this topic. MATERIALS AND METHODS: mRNA and protein expression was examined in 30 breast cancer samples and compared to that of normal adjacent tissue using real time-polymerase chain reaction (PCR) and western blotting. RESULTS: Our results showed that expression of MIG2 and migfilin was significantly reduced in the majority of the breast cancer tissues compared to normal tissues regardless of metastatic status and disease stage. CONCLUSION: Both MIG2 and migfilin are down-regulated in breast cancer.


Subject(s)
Biomarkers, Tumor/metabolism , Breast Neoplasms/metabolism , Breast/metabolism , Cell Adhesion Molecules/metabolism , Cytoskeletal Proteins/metabolism , Membrane Proteins/metabolism , Neoplasm Proteins/metabolism , Biomarkers, Tumor/genetics , Blotting, Western , Breast/pathology , Breast Neoplasms/genetics , Breast Neoplasms/pathology , Carcinoma, Ductal, Breast/genetics , Carcinoma, Ductal, Breast/metabolism , Carcinoma, Ductal, Breast/secondary , Carcinoma, Intraductal, Noninfiltrating/genetics , Carcinoma, Intraductal, Noninfiltrating/metabolism , Carcinoma, Intraductal, Noninfiltrating/secondary , Carcinoma, Lobular/genetics , Carcinoma, Lobular/metabolism , Carcinoma, Lobular/secondary , Cell Adhesion Molecules/genetics , Cytoskeletal Proteins/genetics , Female , Humans , Membrane Proteins/genetics , Neoplasm Invasiveness , Neoplasm Proteins/genetics , Prognosis , RNA, Messenger/genetics , Real-Time Polymerase Chain Reaction , Reverse Transcriptase Polymerase Chain Reaction
5.
Biochem Biophys Res Commun ; 430(2): 494-9, 2013 Jan 11.
Article in English | MEDLINE | ID: mdl-23237804

ABSTRACT

Osteoarthritis (OA) is a debilitating disease of the joints characterized by cartilage degradation but to date there is no available pharmacological treatment to inhibit disease progression neither is there any available biomarker to predict its development. In the present study, we examined the expression level and possible involvement of novel cell-ECM adhesion-related molecules such as Iintegrin Linked Kinase (ILK), PINCH, parvin, Mig-2 and Migfilin in OA pathogenesis using primary human articular chondrocytes from healthy individuals and OA patients. Our findings show that only ILK and Migfilin were upregulated in OA compared to the normal chondrocytes. Interestingly, Migfilin silencing in OA chondrocytes rather exacerbated than ameliorated the osteoarthritic phenotype, as it resulted in even higher levels of catabolic and hypertrophic markers while at the same time induced reduction in ECM molecules such as aggrecan. Furthermore, we also provide a link between Migfilin and ß-catenin activation in OA chondrocytes, showing Migfilin to be inversely correlated with ß-catenin. Thus, the present study emphasizes for the first time to our knowledge the role of Migfilin in OA and highlights the importance of cell-ECM adhesion proteins in OA pathogenesis.


Subject(s)
Cell Adhesion Molecules/metabolism , Chondrocytes/metabolism , Cytoskeletal Proteins/metabolism , Osteoarthritis/metabolism , beta Catenin/biosynthesis , Cell Adhesion Molecules/genetics , Cells, Cultured , Chondrocytes/pathology , Cytoskeletal Proteins/genetics , Disease Progression , Extracellular Matrix/metabolism , Gene Silencing , Humans , Matrix Metalloproteinase 13/biosynthesis , Matrix Metalloproteinase 13/genetics , Osteoarthritis/pathology , Phenotype , RNA, Messenger/biosynthesis , Up-Regulation
6.
Int J Radiat Biol ; 87(6): 609-21, 2011 Jun.
Article in English | MEDLINE | ID: mdl-21627565

ABSTRACT

PURPOSE: Telomerase activity (TA), frequently observed in cancer, compensates for telomere shortening thus preventing cell senescence and conferring resistance to therapy. In the present study, we investigated the expression of human telomerase reverse transcriptase (hTERT) and TA and their regulation, as well as apoptotic rates and correlation with the presence of human epidermal growth factor receptor 2 (HER2), in irradiated tumour-derived breast cancer cells. MATERIALS AND METHODS: In 50 breast cancer tissue samples hTERT mRNA expression and TA were correlated with cell features (HER2, Estrogen and Progesterone Receptor status). Cells from six samples were then irradiated with 10 and 20 Gy; apoptotic rates were measured by flow cytometry, hTERT mRNA expression by real-time polymerase chain reaction and TA by telomeric repeat amplification protocol assay, at 24-144 h post-irradiation. Chromatin immunoprecipitation was performed to investigate hTERT and cellular-myelocytomatosis (c-myc) promoters' activity. HER2 gene knockdown was performed using small interfering RNA technology. RESULTS: hTERT/TA were found increased only in irradiated HER2-positive cells, which were found to be more radioresistant, while HER2 knockdown led to hTERT/TA downregulation. HER2 was found to mediate hTERT expression through activation of Nuclear Factor-kappa B (NF-κB) and c-myc. CONCLUSIONS: The present study suggests that following irradiation, HER2 receptor activates hTERT/telomerase, increasing the breast cancer cells' survival potential, through sequential induction of transcription factors NF-κΒ and c-myc.


Subject(s)
Breast Neoplasms/metabolism , Gene Expression Regulation, Neoplastic , NF-kappa B/metabolism , Proto-Oncogene Proteins c-myc/metabolism , Receptor, ErbB-2/metabolism , Telomerase/metabolism , Apoptosis , Chromatin Immunoprecipitation , Female , Humans , Phenotype , RNA, Messenger/metabolism , Receptors, Estrogen/metabolism , Receptors, Progesterone/metabolism , Telomere/metabolism , Time Factors
7.
Exp Biol Med (Maywood) ; 236(1): 70-6, 2011 Jan.
Article in English | MEDLINE | ID: mdl-21239736

ABSTRACT

Although leptin has been found to be implicated in obesity-related breast carcinogenesis in postmenopausal women, the molecular mechanisms involved are yet to be defined. Recently, the antiapoptotic gene survivin has been recognized as a target gene for leptin in breast cancer. The aim of this study was to investigate the effect of leptin on the expression of survivin and on the transcriptional activity of its promoter in MCF-7 breast cancer cells. We also studied the potential involvement of SOCS-3 (a negative regulator of leptin's main signaling pathway JAK2/STAT3) in the expression of leptin-mediated survivin. Our results showed a significant increase in the mRNA (dose-dependent increase of 40-70%) and protein expression levels of survivin 24 h post-leptin treatment, which was followed by a significant decrease at 48 and 72 h (of 60-70%). In accordance, a chromatin immunoprecipitation assay revealed an initial strong binding of STAT3 to the survivin promoter, which was no longer detected after 24 h. Myc/mad/max network proteins and histone H3 acetylation status were not found to contribute to the expression of leptin-mediated survivin. Furthermore, a protein immunoprecipitation assay detected an enhanced SOCS-3 binding to the long isoform of leptin's receptor (Ob-Rb) 48 and 72 h after leptin administration, thus conferring inhibition to leptin signaling. In conclusion, our findings suggest, for the first time to our knowledge, that the effect of leptin on the antiapoptotic gene survivin is limited by the inhibitory role of SOCS-3 in the leptin-activated JAK2/STAT3 signaling pathway in MCF-7 breast cancer cells.


Subject(s)
Breast Neoplasms/metabolism , Leptin/pharmacology , Microtubule-Associated Proteins/metabolism , Suppressor of Cytokine Signaling Proteins/physiology , Apoptosis/drug effects , Apoptosis/physiology , Blotting, Western , Breast Neoplasms/physiopathology , Cell Line, Tumor , Chromatin Immunoprecipitation , Dose-Response Relationship, Drug , Female , Humans , Inhibitor of Apoptosis Proteins , Janus Kinase 2/metabolism , Janus Kinase 2/physiology , Microtubule-Associated Proteins/drug effects , Microtubule-Associated Proteins/physiology , Promoter Regions, Genetic/physiology , Reverse Transcriptase Polymerase Chain Reaction , STAT3 Transcription Factor/metabolism , STAT3 Transcription Factor/physiology , Signal Transduction/drug effects , Signal Transduction/physiology , Suppressor of Cytokine Signaling 3 Protein , Suppressor of Cytokine Signaling Proteins/drug effects , Survivin
8.
J Cell Mol Med ; 15(7): 1542-50, 2011 Jul.
Article in English | MEDLINE | ID: mdl-20716114

ABSTRACT

Radiotherapy is an important treatment modality against cancer resulting in apoptosis and inhibition of cell growth. Survivin is an important cancer biomarker conferring to tumour cells increased survival potential by inhibiting apoptosis. In the present study, we investigated the implication of breast cancer cells features, as hormone receptors and p53 status, in the radio-resistance of breast cancer cells and in the regulation of survivin's expression by nuclear factor (NF)-κB and c-myc. Six breast cancer cell lines Michigan Cancer Foundation (MCF-7), MCF-7/Human Epidermal Growth Factor Receptor (HER)2, M. D. Anderson - Metastatic Breast (MDA-MB-231), SK-BR-3, BT-474 and Human Breast Lactating (HBL-100) were irradiated and cell viability as well as cell cycle distribution were evaluated by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and flow cytometry, respectively. Survivin mRNA and protein levels were evaluated by real time PCR and Western blot analysis. Survivin and HER2 gene knockdown was performed with siRNA technology and investigation of transcription factors binding to survivin and c-myc gene promoters was assessed by chromatin immunoprecipitation. Student's t-test and F-statistics were used for statistical evaluation. Our results demonstrated that only HER2(+) breast cancer cells up-regulated survivin upon irradiation, whereas HER2 knockdown in HER2(+) cells led to survivin's down-regulation. Survivin and especially HER2 knockdown abolished the observed G2/M cell cycle checkpoint and reduced the radio-resistance of HER2 overexpressing breast cancer cells. Additionally, HER2 was found to regulate survivin's expression through NF-κB and c-myc transcription factors. This study revealed the significance of HER2 in the radio-resistance of HER2(+) breast cancer cells through induction of transcription factors NF-κB and c-myc, leading to activation of survivin, a downstream target oncogene preventing apoptosis.


Subject(s)
Breast Neoplasms/pathology , Breast Neoplasms/radiotherapy , Gene Expression Regulation, Neoplastic , Inhibitor of Apoptosis Proteins/metabolism , NF-kappa B/metabolism , Proto-Oncogene Proteins c-myc/metabolism , Receptor, ErbB-2/metabolism , Cell Line, Tumor/radiation effects , Female , Gene Knockdown Techniques , Humans , Inhibitor of Apoptosis Proteins/genetics , NF-kappa B/genetics , Promoter Regions, Genetic , Proto-Oncogene Proteins c-myc/genetics , Receptor, ErbB-2/genetics , Survivin , Transcription Factors/metabolism
9.
BMC Cancer ; 10: 442, 2010 Aug 19.
Article in English | MEDLINE | ID: mdl-20723213

ABSTRACT

BACKGROUND: Numerous epidemiological studies have documented that obesity is associated with hepatocellular carcinoma (HCC). The aim of this study was to investigate the biological actions regulated by leptin, the obesity biomarker molecule, and its receptors in HCC and the correlation between leptin and human telomerase reverse transcriptase (hTERT), a known mediator of cellular immortalization. METHODS: We investigated the relationship between leptin, leptin receptors and hTERT mRNA expression in HCC and healthy liver tissue samples. In HepG2 cells, chromatin immunoprecipitation assay was used to study signal transducer and activator of transcription-3 (STAT3) and myc/mad/max transcription factors downstream of leptin which could be responsible for hTERT regulation. Flow cytometry was used for evaluation of cell cycle modifications and MMP1, 9 and 13 expression after treatment of HepG2 cells with leptin. Blocking of leptin's expression was achieved using siRNA against leptin and transfection with liposomes. RESULTS: We showed, for the first time, that leptin's expression is highly correlated with hTERT expression levels in HCC liver tissues. We also demonstrated in HepG2 cells that leptin-induced up-regulation of hTERT and TA was mediated through binding of STAT3 and Myc/Max/Mad network proteins on hTERT promoter. We also found that leptin could affect hepatocellular carcinoma progression and invasion through its interaction with cytokines and matrix mettaloproteinases (MMPs) in the tumorigenic microenvironment. Furthermore, we showed that histone modification contributes to leptin's gene regulation in HCC. CONCLUSIONS: We propose that leptin is a key regulator of the malignant properties of hepatocellular carcinoma cells through modulation of hTERT, a critical player of oncogenesis.


Subject(s)
Carcinoma, Hepatocellular/pathology , Leptin/pharmacology , Liver Neoplasms/pathology , Liver/pathology , Telomerase/metabolism , Adult , Aged , Aged, 80 and over , Apoptosis/drug effects , Blotting, Western , Carcinoma, Hepatocellular/drug therapy , Carcinoma, Hepatocellular/enzymology , Cell Cycle , Cell Line, Tumor , Cell Proliferation/drug effects , Chromatin Immunoprecipitation , Female , Flow Cytometry , Gene Expression Regulation, Neoplastic , Humans , Immunoenzyme Techniques , Liver/metabolism , Liver Neoplasms/drug therapy , Liver Neoplasms/enzymology , Male , Middle Aged , Promoter Regions, Genetic/genetics , Proto-Oncogene Proteins c-myc/genetics , Proto-Oncogene Proteins c-myc/metabolism , RNA, Messenger/genetics , RNA, Small Interfering/pharmacology , Receptors, Leptin/genetics , Receptors, Leptin/metabolism , Reverse Transcriptase Polymerase Chain Reaction , STAT3 Transcription Factor/genetics , STAT3 Transcription Factor/metabolism , Telomerase/antagonists & inhibitors , Telomerase/genetics , Up-Regulation
10.
Int J Oncol ; 35(5): 1141-9, 2009 Nov.
Article in English | MEDLINE | ID: mdl-19787269

ABSTRACT

Cancer cell characteristics may play a pivotal role in the response to therapy by activating or deactivating different molecular pathways. In the present study, we investigated the implication of breast cancer cell features, such as HER2 and p53 in the activation of telomerase upon exposure to ionizing radiation. Telomerase is among the most important cancer biomarkers, conferring to tumor cells unlimited proliferative capacity, increased survival potential and resistance to several types of cellular stress. We investigated possible mechanisms regulating telomerase in six irradiated breast cancer cell lines (MCF-7, MCF-7/HER2, MDA-MB-231, SK-BR-3, BT-474 and HBL-100) differing in their HER2, p53 and ERalpha status. hTERT mRNA expression was evaluated by real-time PCR and telomerase activity by the TRAP assay. HER2, c-myc, p53 and p21 protein levels were evaluated by Western blotting. Silencing of hTERT and HER2 was achieved by small interfering RNA technology. Chromatin immunoprecipitation was used to evaluate H3 histone acetylation status, as well as myc/mad/max and p53 transcription factors interaction with the hTERT promoter. Our results showed for the first time, that only HER2-positive cells, independently of their p53 status, upregulated hTERT/telomerase, while knockdown of hTERT increased radio-sensitivity. Knockdown of HER2 also led to increased radio-sensitivity and downregulation of hTERT/telomerase. We also demonstrated that c-myc and mad1 regulate hTERT expression in all irradiated breast cancer cells. We conclude, for the first time, that HER2 phenotype upregulates hTERT through c-myc activation and confers radio-resistance to breast cancer cells.


Subject(s)
Breast Neoplasms/genetics , Gene Expression/radiation effects , Radiation Tolerance/genetics , Receptor, ErbB-2/genetics , Telomerase/biosynthesis , Tumor Suppressor Protein p53/genetics , Biomarkers, Tumor/radiation effects , Blotting, Western , Breast Neoplasms/metabolism , Cell Cycle Proteins/genetics , Cell Cycle Proteins/metabolism , Cell Cycle Proteins/radiation effects , Cell Line, Tumor , Chromatin Immunoprecipitation , Female , Gene Expression Regulation, Neoplastic/genetics , Gene Expression Regulation, Neoplastic/radiation effects , Humans , Nuclear Proteins/genetics , Nuclear Proteins/metabolism , Nuclear Proteins/radiation effects , Proto-Oncogene Proteins c-myc/genetics , Proto-Oncogene Proteins c-myc/metabolism , Proto-Oncogene Proteins c-myc/radiation effects , RNA, Small Interfering , Receptor, ErbB-2/metabolism , Receptor, ErbB-2/radiation effects , Reverse Transcriptase Polymerase Chain Reaction , Telomerase/genetics , Telomerase/radiation effects , Tumor Suppressor Protein p53/metabolism , Tumor Suppressor Protein p53/radiation effects , Up-Regulation
11.
J Clin Pediatr Dent ; 29(2): 175-8, 2005.
Article in English | MEDLINE | ID: mdl-15719925

ABSTRACT

Pediatric oral pathology encompasses a wide clinical spectrum of local and systemic diseases. The purpose of this study was to evaluate the clinical characteristics of oral soft tissue lesions in Greek children and adolescents up to 18 years old. Data available through a 32 year old period revealed that among the 1040 cases analyzed, benign lesions, mainly cysts, inflammatory lesions and reactive hyperplasias, were the most common causes for seeking dental advice during childhood.


Subject(s)
Mouth Diseases/epidemiology , Patient Acceptance of Health Care/statistics & numerical data , Soft Tissue Neoplasms/epidemiology , Adolescent , Child , Child, Preschool , Female , Greece/epidemiology , Humans , Infant , Male , Retrospective Studies
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