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1.
Epidemiol Infect ; 145(2): 326-328, 2017 01.
Article in English | MEDLINE | ID: mdl-27751198

ABSTRACT

The results of the study reported here are part of an ongoing integrated research programme aimed at producing additional, robust, evidence on the genetic resistance to classical scrapie in goats, with particular reference to codon 146. The study targeted animals aged ⩾6 years, which were born and raised in infected herds and were being culled for management reasons. A total of 556 animals were tested, and all positive animals (n = 117) were of the susceptible NN genotype. A total of 246 goats heterozygous or homozygous for putatively resistant alleles (S146 and D146) were screened with no positive results. The outcome of this study supports the hypothesis that the D146 and S146 alleles could be used as the basis for a nationwide strategy for breeding for resistance in the Cypriot goat population.


Subject(s)
Disease Resistance , Goat Diseases/genetics , Mutant Proteins/genetics , Prion Proteins/genetics , Scrapie/genetics , Amino Acid Substitution , Animals , Aspartic Acid/genetics , Cyprus , Goats , Mutation, Missense , Serine/genetics
3.
Epidemiol Infect ; 143(6): 1304-10, 2015 Apr.
Article in English | MEDLINE | ID: mdl-25140573

ABSTRACT

Previous studies have shown the association between the polymorphisms serine (S) or aspartic acid (D) at codon 146 of the PRNP gene and resistance to scrapie. All goats aged >12 months (a total of 1075 animals) from four herds with the highest prevalence of scrapie in the country were culled and tested, of which 234 (21·7%) were positive by either the rapid test or immunohistochemistry (IHC) for any of the tissues tested. The odds of scrapie infection occurring in NN146 goats was 101 [95% credible interval (CrI) 19-2938] times higher than for non-NN146 or unknown genotypes. IHC applied to lymphoreticular tissue produced the highest sensitivity (94%, 95% CrI 90-97). The presence of putatively resistant non-NN146 alleles in the Cypriot goat population, severely affected by scrapie, provides a potential tool to reduce/eradicate scrapie provided that coordinated nationwide breeding programmes are implemented and maintained over time.


Subject(s)
Goat Diseases/epidemiology , PrPSc Proteins/genetics , Scrapie/epidemiology , Animals , Codon/genetics , Cyprus/epidemiology , Disease Susceptibility/veterinary , Female , Goats , Male , Polymorphism, Genetic/genetics , Prevalence
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