[Evaluation of the effectiveness of antibacterial substances in treating an experimental form of bubonic plague in monkeys]. / Otsenka éffektivnosti antibakterial'nykh preparatov pri lechenii éksperimental'noi bubonnoi formy chumy u obez'ian.

The modelling of glandular plague and selection of the conditions for estimating the efficacy of new antibacterials for the treatment of the infection were performed on hamadryads (baboons). The experiments showed that the average LD50 of the culture of a highly virulent strain of Yersinia pestis on its subcutaneous administration to the animals was 2089 viable microbes. In 18 per cent of the episodes the experimental glandular plague in the animals was complicated by secondary plague pneumonia. Subcutaneous administration of 2 x 10(7) viable microbial cell of the plague pathogen caused acute sepsis and the animal death. The treatment of the experimental glandular plague in the hamadryads demonstrated that new antibacterials such as amikacin, netilmicin, ceftriaxone, cefotaxime, ceftizoxime, doxycycline, rifampicin, ofloxacin and ciprofloxacin were not inferior in their efficacy to streptomycin and tetracycline successfully used in the therapy of patients with plague.

[Surgical treatment of pyo-necrotic forms of diabetic foot]. / Khirurgicheskoe lechenie gnoino-nekroticheskoi formy diabeticheskoi stopy.

The authors present the experience of combined surgical treatment of 94 patients with complicated "diabetic foot". In the majority of patients (92.8%) there was ischemic form of diabetic foot. Wide usage of staged necrectomies as treatment modalities, preferable use of economical amputations and exarticulations, continuous intensive intraarterial infusion (heparine, desaggregants, antibiotics, insulin suspension) contributed to preservation of the limbs in 82.6% of cases.

[Standardization of conditions for the evaluation of effectiveness of antibacterial drugs in pneumonic plague in sacred baboons]. / Standartizatsiia uslovii otsenki éffektivnosti antibakterial'nykh preparatov pri lechenii legochnoi chumy u obez'ian pavianov gamadrilov.

The signs of pneumonic plague in sacred baboons infected by aerosol are: fever, hurried breathing, depression and constantly increasing bacteremia. Some infected animals isolate the plague microbes while coughing and thus could be a source of the infection. By the clinical and pathomorphological signs, pneumonic plague in sacred baboons is similar to that in humans which makes it possible to use the animals in the development of schemes for special prophylaxis and treatment of the disease. In efficacy estimation of antibacterial drugs sacred baboons should be infected by aerosol by highly virulent strains of Y. pestis in doses of 1.10(4)-1.10(5) live microbes. The treatment of the animals should be started from the moment of the rectal temperature increase to 39.5 degrees C or higher after collecting the blood specimens for the bacteriological tests. It was shown that a two-day course of the treatment with antibacterial drugs was not efficient in the animals with pneumonic plague. The use of streptomycin, gentamicin, netilmicin or ciprofloxacin for 7 days cured all the infected animals. The use of streptomycin in the therapeutic doses was not efficient in the animals whose blood specimens of 1 cm3 contained 4.10(4) or more plague microbes by the moment when the treatment was started.

[Assessment of the effectiveness of different methoods of immunization with live plague vaccine EB in aerosol infections]. / Otsenka éffektivnosti razlichnykh metodov immunizatsii chumnoi zhivoi vaktsinoi EB pri aérozol'nom zarazhenii.

The work deals with the results of the comparative evaluation of the effectiveness of vaccines developed at the Sanitary Research Institute (Zagorsk) and the Mechnikov Research Institute for Vaccines and Sera (Moscow), as well as two methods of immunization against plague, by inhalation and subcutaneous injection, under the conditions of aerosol infection. The immunogenic effectiveness of both vaccines, when evaluated in terms of LD50, was shown to be approximately the same, but the animals immunized by the inhalation method with the vaccine developed at the Sanitary Research Institute proved to be less susceptible to infection than those immunized with the vaccine developed at the Mechnikov Research Institute for Vaccines and Sera in Moscow. After immunization by the inhalation method the vaccine developed at the Sanitary Research Institute rendered more effective protection (3- to 4-fold) against aerosol infection than after immunization by subcutaneous injection. The animals immunized by the inhalation method proved to be capable of surviving plague in the primary pneumonic form.