Effects of long-term exposure to elevated CO(2) conditions in slow-growing plants using a (12)C-enriched CO(2)-labelling technique.

Despite their relevancy, long-term studies analyzing elevated CO(2) effect in plant production and carbon (C) management on slow-growing plants are scarce. A special chamber was designed to perform whole-plant above-ground gas-exchange measurements in two slow-growing plants (Chamaerops humilis and Cycas revoluta) exposed to ambient (ca. 400 micromol mol(-1)) and elevated (ca. 800 micromol mol(-1)) CO(2) conditions over a long-term period (20 months). The ambient isotopic (13)C/(12)C composition (delta(13)C) of plants exposed to elevated CO(2) conditions was modified (from ca. -12.8 per thousand to ca. -19.2 per thousand) in order to study carbon allocation in leaf, shoot and root tissues. Elevated CO(2) increased plant growth by ca. 45% and 60% in Chamaerops and Cycas, respectively. The whole-plant above-ground gas-exchange determinations revealed that, in the case of Chamaerops, elevated CO(2) decreased the photosynthetic activity (determined on leaf area basis) as a consequence of the limited ability to increase C sink strength. On the other hand, the larger C sink strength (reflected by their larger CO(2) stimulatory effect on dry mass) in Cycas plants exposed to elevated CO(2) enabled the enhancement of their photosynthetic capacity. The delta(13)C values determined in the different plant tissues (leaf, shoot and root) suggest that Cycas plants grown under elevated CO(2) had a larger ability to export the excess leaf C, probably to the main root. The results obtained highlighted the different C management strategies of both plants and offered relevant information about the potential response of two slow-growing plants under global climate change conditions.

Assessing the stable carbon isotopic composition of intercellular CO2 in a CAM plant using gas chromatography-combustion-isotope ratio mass spectrometry.

Most of the literature focused on internal CO(2) (Ci) determinations in plants has used indirect methods based on gas-exchange estimations. We have developed a new method based on the capture of internal air gas samples and their analysis by gas chromatography-combustion-isotope ratio mass spectrometry (GC-C-IRMS). This method provided a direct measure of intercellular CO(2) concentrations combined with stable carbon isotopic composition in O. ficus-indica plants. Plants were grown at both ambient and elevated CO(2) concentration. During the day period, when the stomata are closed, the Ci was high and was very (13)C-enriched in both ambient and elevated CO(2)-grown plants, reflecting Rubisco's fractionation (this plant enzyme has been shown to discriminate by 29 per thousand, in vitro, against (13)CO(2)). Other enzyme fractionations involved in C metabolism in plants, such as carbonic anhydrase, could also be playing an important role in the diurnal delta(13)C enrichment of the Ci. During the night, when stomata are open, Ci concentrations were higher in elevated (and the corresponding delta(13)C values were more (13)C-depleted) than in ambient CO(2)-grown plants.

Respiratory carbon metabolism in the high mountain plant species Ranunculus glacialis.

Very little is known about the primary carbon metabolism of the high mountain plant Ranunculus glacialis. It is a species with C3 photosynthesis, but with exceptionally high malate content in its leaves, the biological significance of which remains unclear. 13C/12C-isotope ratio mass spectrometry (IRMS) and 13C-nuclear magnetic resonance (NMR) labelling were used to study the carbon metabolism of R. glacialis, paying special attention to respiration. Although leaf dark respiration was high, the temperature response had a Q10 of 2, and the respiratory quotient (CO2 produced divided by O2 consumed) was nearly 1, indicating that the respiratory pool is comprised of carbohydrates. Malate, which may be a large carbon substrate, was not respired. However, when CO2 fixed by photosynthesis was labelled, little labelling of the CO2 subsequently respired in the dark was detected, indicating that: (i) most of the carbon recently assimilated during photosynthesis is not respired in the dark; and (ii) the carbon used for respiration originates from (unlabelled) reserves. This is the first demonstration of such a low metabolic coupling of assimilated and respired carbon in leaves. The biological significance of the uncoupling between assimilation and respiration is discussed.