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1.
Sci Total Environ ; 901: 165920, 2023 Nov 25.
Article in English | MEDLINE | ID: mdl-37527721

ABSTRACT

There is growing evidence that poly and perfluoroalkyl substances (PFAS) exposure leads to the disruption of thyroid hormones including thyroxine (T4) and triiodothyronine (T3), and may affect telomeres, repetitive nucleotide sequences which protect chromosome ends. Many seabird species are long-lived top predators thus exhibit high contaminant levels, and PFAS-disrupting effects on their physiology have been documented especially in relation to the endocrine system in adults. On the contrary, studies on the developmental period (i.e., chicks), during which exposure to environmental contaminants may have a greater impact on physiological traits, remain scarce to this date. We carried out a multi-species study with the aim to assess whether and to which extent chicks of four gull species (herring gull, great and lesser black-backed gull, yellow-legged gull) in South Western France are contaminated by PFAS, and to bring further evidence about their potential physiological consequences. Linear PFOS showed concentrations of concern as it was generally >10 times higher than the other PFAS, and exceeded a threshold toxicity level (calculated from previous studies in birds) in almost all sampled chicks. Nonetheless, in herring gull male chicks, total T3 levels were significantly and negatively associated with perfluorodecanoate (PFDA) and perfluorododecanoate (PFDoDA) and positively associated with perfluorotetradecanoate (PFTeDA) in female chicks. Total T3 levels were also positively associated with PFDoDA in great black backed gull male chicks and with perfluorotridecanoate (PFTrDA) in lesser black backed gull chicks. In lesser and great black-backed gulls, both females and males showed significant negative associations between several PFAS and their body condition, and a positive association between telomere length and L-PFOS in the yellow-legged gull was also found. These results corroborate previous findings and need to be further explored as they suggest that PFAS may interfere with the physiological status of chicks during the developmental period, potentially inducing long-lasting consequences.

2.
Ecotoxicol Environ Saf ; 225: 112720, 2021 Dec 01.
Article in English | MEDLINE | ID: mdl-34509163

ABSTRACT

The last remaining population of European sturgeon (Acipenser sturio) lives in the Gironde-Garonne-Dordogne (France) catchment (GGD). Captive young individuals are released into the GGD hydrosystem each year, as part of a restocking programme. This study aims to assess the health status of juveniles A. sturio to current conditions in the GGD hydrosystem, to evaluate their capacity to survive and grow in a moderately anthropized ecosystems. 3-month-old farmed sturgeons were exposed for one month in experimental conditions that mimic the environmental conditions in the Garonne and Dordogne rivers, followed by five months of depuration. After one month of exposure, fish exposed to Dordogne and Garonne waters bioaccumulated higher levels of metals and persistent organic pollutants, displayed a reduced hepato-somatic index, and had depleted levels of lipids and glycogen content in their liver, when compared with the Reference group. However, metabolic and swimming performance, as well as the costs of swimming were not impaired. After the 5 months depuration, a significant decrease of K was observed for all exposure conditions. HSI also decreased with time. The overall health status and adaptive capacity of juvenile A. sturio appeared to be maintained over the experimental 6 months' period. Juveniles of A. sturio seem to have the adaptive capacity to survive and grow in the GGD hydrosystem, after being released as part of a restocking programme.


Subject(s)
Persistent Organic Pollutants , Rivers , Animals , Ecosystem , Fishes , Humans , Infant , Metals
3.
Sci Total Environ ; 765: 144611, 2021 Apr 15.
Article in English | MEDLINE | ID: mdl-33385816

ABSTRACT

Per- and poly-fluoroalkyl substances (PFAS) raised increasing concerns over the past years due to their persistence and global distribution. Understanding their occurrence in the environment and their disruptive effect on the physiology of humans and wildlife remains a major challenge in ecotoxicological studies. Here, we investigate the occurrence of several carboxylic and sulfonic PFAS in 105 individuals of three seabird species (27 great black-backed gull Larus marinus; 44 lesser black-backed gull Larus fuscus graellsii; and 34 European herring gull Larus argentatus) from South western France. We further estimated the relationship between plasma concentrations of PFAS and i) the body condition of the birds and ii) plasma concentrations of thyroid hormone triiodothyronine (TT3). We found that great and lesser black-backed gulls from South Western France are exposed to PFAS levels comparable to highly contaminated species from other geographical areas, although major emission sources (i.e. related to industrial activities) are absent in the region. We additionally found that PFAS are negatively associated with the body condition of the birds in two of the studied species, and that these results are sex-dependent. Finally, we found positive associations between exposure to PFAS and TT3 in the great black-backed gull, suggesting a potential disrupting mechanism of PFAS exposure. Although only three years of data have been collected, we investigated PFAS trend over the study period, and found that great black-backed gulls document an increasing trend of plasma PFAS concentration from 2016 to 2018. Because PFAS might have detrimental effects on birds, French seabird populations should be monitored since an increase of PFAS exposure may impact on population viability both in the short- and long-term.


Subject(s)
Charadriiformes , Animals , Birds , France , Humans , Thyroid Hormones
4.
Environ Pollut ; 253: 1117-1125, 2019 Oct.
Article in English | MEDLINE | ID: mdl-31434189

ABSTRACT

Bed sediments and a dated sediment core were collected upstream and downstream from the city of Lyon (France) to assess the spatial and temporal trends of contamination by per- and polyfluoroalkyl substances (PFASs) in this section of the Rhône River. Upstream from Lyon, concentrations of total PFASs (ΣPFASs) in sediments are low (between 0.19 and 2.6 ng g-1 dry weight - dw), being characterized by a high proportion of perfluorooctane sulfonate (PFOS). Downstream from Lyon, and also from a fluoropolymer manufacturing plant, ΣPFASs concentrations reach 48.7 ng g-1 dw. A gradual decrease of concentrations is reported at the coring site further downstream (38 km). Based on a dated sediment core, the temporal evolution of PFASs is reconstructed from 1984 to 2013. Prior to 1987, ΣPFASs concentrations were low (≤2 ng g-1 dw), increasing to a maximum of 51 ng g-1 dw in the 1990s and then decreasing from 2002 to the present day (∼10 ng g-1 dw). In terms of the PFAS pattern, the proportion of perfluoroalkyl sulfonic acids (PFSAs) has remained stable since the 1980s (∼10%), whereas large variations are reported for carboxylic acids (PFCAs). Long chain- (C > 8) PFCAs characterized by an even number of perfluorinated carbons represent about 74% of the total PFAS load until 2005. However, from 2005 to 2013, the relative contribution of long chain- (C > 8) PFCAs with an odd number of perfluorinated carbons reaches 80%. Such changes in the PFAS pattern likely highlight a major shift in the industrial production process. This spatial and retrospective study provides valuable insights into the long-term contamination patterns of PFAS chemicals in river basins impacted by both urban and industrial activities.


Subject(s)
Environmental Monitoring , Fluorocarbons/analysis , Geologic Sediments/chemistry , Water Pollutants, Chemical/analysis , Water Pollution, Chemical/statistics & numerical data , Alkanesulfonic Acids , Carboxylic Acids , France , Manufacturing and Industrial Facilities , Retrospective Studies , Rivers/chemistry , Sulfonic Acids/analysis
5.
Chemosphere ; 87(11): 1335-40, 2012 Jun.
Article in English | MEDLINE | ID: mdl-22405722

ABSTRACT

In the context of massive summer mortality events of the Pacific oyster Crassostrea gigas, the aim of this study was to investigate the early effects on genes, enzymes and haemocyte parameters implicated in immune defence mechanisms in C. gigas oysters exposed to a potentially hostile environment, i.e. to an herbicide alone or within a mixture. Following 2 h of exposure to the herbicide diuron at 1 µg L(-1), the repression of different genes implicated in immune defence mechanisms in the haemocytes and the inhibition of enzyme activities, such as laccase-type phenoloxidase (PO) in the plasma, were observed. The inhibition of superoxide dismutase (SOD) activity in the plasma was also observed after 6 and 24 h of exposure. In the mixture with the herbicides diuron and isoproturon, and the pharmaceutical ibuprofen, catecholase-type PO activity in the plasma and the percentage of phagocytosis in the haemocytes were reduced after 6 h of exposure. Our results showed that early effects on molecular, biochemical and cellular parameters can be detected in the presence of diuron alone or within a mixture, giving an insight of its potential effect in situations that can be found in natural environments, i.e. relatively high concentrations for short periods of time.


Subject(s)
Crassostrea/drug effects , Diuron/toxicity , Herbicides/toxicity , Ibuprofen/toxicity , Phenylurea Compounds/toxicity , Water Pollutants, Chemical/toxicity , Animals , Crassostrea/enzymology , Hemocytes/drug effects , Hemocytes/immunology , Monophenol Monooxygenase/metabolism , Phagocytosis , Seawater/chemistry , Superoxide Dismutase/antagonists & inhibitors , Superoxide Dismutase/blood
6.
J Microbiol Methods ; 65(2): 324-34, 2006 May.
Article in English | MEDLINE | ID: mdl-16185781

ABSTRACT

A method to determine the individual lag time (lag) distributions of immobilized bacteria was presented. The method was based on the image analysis of the bacterial colony growth. The lag distributions were retrieved from the distributions of the detection times (Td) required to form macroscopically visible colonies. Using this method, the lag distributions on agar for Listeria monocytogenes cells previously subjected to two situations reproducing conditions encountered during the contamination of cheese, were determined. The results were presented and compared with lag distributions obtained with an established method based on the time to detection of turbidity in broth. An original method to retrieve lag in broth and agar without any knowledge of the growth rate was also proposed. In order not to bias the distributions of lag on agar the impact of spatial separation between colonies on colony growth rates was quantified. Means and standard deviations of lag distributions for the two different stresses were found to be similar in broth and on agar. Extreme Value type II distributions fitted the best the different datasets of lag distributions.


Subject(s)
Image Processing, Computer-Assisted/methods , Listeria monocytogenes/cytology , Listeria monocytogenes/growth & development , Agar , Cells, Immobilized , Culture Media , Heat-Shock Response
7.
Appl Environ Microbiol ; 71(6): 2940-8, 2005 Jun.
Article in English | MEDLINE | ID: mdl-15932988

ABSTRACT

The effects of nine common food industry stresses on the times to the turbidity (T(d)) distribution of Listeria monocytogenes were determined. It was established that the main source of the variability of T(d) for stressed cells was the variability of individual lag times. The distributions of T(d) revealed that there was a noticeable difference in response to the stresses encountered by the L. monocytogenes cells. The applied stresses led to significant changes of the shape, the mean, and the variance of the distributions. The variance of T(d) of wells inoculated with single cells issued from a culture in the exponential growth phase was multiplied by at least 6 and up to 355 for wells inoculated with stressed cells. These results suggest stress-induced variability may be important in determining the reliability of predictive microbiological models.


Subject(s)
Heat-Shock Response , Listeria monocytogenes/growth & development , Listeria monocytogenes/physiology , Culture Media , Food-Processing Industry , Models, Biological , Time Factors
8.
Avian Dis ; 47(1): 163-8, 2003.
Article in English | MEDLINE | ID: mdl-12713172

ABSTRACT

In order to estimate the contribution of Salmonella in the persistence of this bacterium in chicks, we compared the persistence of a Salmonella enteritidis strain and its plasmid-cured variant in a chicken asymptomatic carrier state model. After oral inoculation, colonization with the plasmid-cured strain was significantly reduced (P < 0.001) in the ceca of chicks from the third week postinoculation and persisted for a shorter period than the wild-type strain. Moreover, numbers of S. enteriditis-infected livers were also significantly lower (P < 0.01) for the plasmid-cured strain compared with the wild-type strain from the third to the seventh week postinoculation. No difference in spleen colonization was observed. These results did not correlate with any in vitro difference in attachment, entry to, or intracellular multiplication of bacteria within intestinal or macrophage avian cell lines.


Subject(s)
Cecum/microbiology , Chickens/microbiology , Plasmids/analysis , Plasmids/genetics , Salmonella enteritidis/genetics , Salmonella enteritidis/isolation & purification , Animals , Bacterial Adhesion , Cell Line , Enterocytes/microbiology , Liver/microbiology , Macrophages/microbiology , Phagocytosis , Salmonella enteritidis/classification , Spleen/microbiology , Time Factors
9.
Int J Food Microbiol ; 68(1-2): 33-44, 2001 Aug 15.
Article in English | MEDLINE | ID: mdl-11545218

ABSTRACT

Some Listeria monocytogenes strains not related to clinical cases have been found to exhibit a low virulence level in mice as well as in an in vitro test using Caco-2 cells. The purpose of this study was to validate a new in vitro test of virulence based on a plaque-forming assay (PFA) using a HT-29 cell monolayer with 118 Listeria strains. The use of HT-29 cells in 96-well tissue culture plates allowed the testing of 30 strains per day and providing results in 24 h. In addition. statistical analyses demonstrated the reproducibility and repeatability of the PFA. No quantitative relationship was observed between the virulence of the strains and the hemolytic titer or the cytotoxic effects on HT-29 cells. In contrast, good agreement was observed between virulence assessed after subcutaneous (SC) infection and virulence obtained by PFA. Three groups of L. monocytogenes strains (avirulent, hypovirulent and fully virulent) were established by comparison of the clinical origin of the strains, the number of immunocompetent contaminated mice and the numbers of Listeria strains recovered in the spleen after SC infection. With one exception, i.e. a clinical case of L. seeligeri (sensitivity 0.98), the PFA successfully detected the virulent strains only (specificity 1). Decision-tree algorithms performed by SAS and S-Plus demonstrated that this tissue culture assay discriminated between the avirulent and hypovirulent strains and the virulent strains. This test could therefore be an alternative to in vivo tests, allowing grading of virulence.


Subject(s)
Immunocompromised Host/immunology , Listeria monocytogenes/pathogenicity , Listeriosis/microbiology , Virulence/physiology , Animals , Caco-2 Cells , Female , HT29 Cells , Humans , Listeria monocytogenes/classification , Listeria monocytogenes/physiology , Male , Mice , Reproducibility of Results , Sensitivity and Specificity , Tumor Cells, Cultured , Viral Plaque Assay
10.
J Food Prot ; 64(4): 470-5, 2001 Apr.
Article in English | MEDLINE | ID: mdl-11307881

ABSTRACT

Edible cellulosic films made with hydroxypropylmethylcellulose (HPMC) have proven to be inadequate moisture barriers. To improve its water vapor barrier properties, different hydrophobic compounds were incorporated into the HPMC matrix. Some fatty acids and derivatives were included into the film-forming solution prior to film formation. Stearic acid was chosen because of its high capacity to reduce significantly the water vapor transmission rate. Antimicrobial activity of edible HPMC film was obtained by the incorporation of nisin into the film-forming solution. Nisin is an antimicrobial peptide effective against gram-positive bacteria. The inhibitory activity of this bacteriocin was tested for inhibition of Listeria innocua and Staphylococcus aureus. The use of stearic acid was observed to reduce the inhibitory activity of active HPMC film against both selected strains. This phenomenon may be explained by electrostatic interactions between the cationic nisin and the anionic stearic acid. Further studies showed that antimicrobial activity of film varied with the nature of the hydrophobic compound incorporated, in decreasing order: film without lipid, methylstearate film, and stearic acid film. This corroborated the idea of electrostatic interactions.


Subject(s)
Anti-Bacterial Agents/pharmacology , Food Packaging , Food Preservation , Listeria/drug effects , Staphylococcus aureus/drug effects , Ethers/pharmacology , Fatty Acids/pharmacology , Nisin/pharmacology , Static Electricity
11.
FEMS Microbiol Lett ; 174(2): 265-72, 1999 May 15.
Article in English | MEDLINE | ID: mdl-10339818

ABSTRACT

Expression of the PrfA-controlled virulence gene hly (encoding the pore-forming cytolysin listeriolysin) is down-regulated by readily metabolized carbon sources in Listeria monocytogenes. We isolated a Tn917-insertional mutant of L. monocytogenes (strain LO28), which expressed a hemolytic phenotype in the presence of cellobiose. Using hly fusions to luxAluxB genes, we show that hly expression was derepressed in the presence of cellobiose at the transcriptional level. Surprisingly, hly expression was still repressed by glucose, as observed for the parental strain. Genetic analysis of the Tn917-flanking regions indicated that the transposon had inserted in a non-coding region located between two genes in opposite orientations. These two newly identified genes were designated orfA and mdrL. The insertion occurred immediately upstream of orfA, likely into its promoter region. Transcriptional analysis of orfA and mdrL revealed that Tn917 had abolished orfA expression whereas it had activated expression of mdrL. orfA encodes a putative protein of 176 amino acids homologous to YfiO of Bacillus subtilis (28% identity), a protein of unknown function. mdrL codes for a putative protein of 398 amino acids homologous to Bmr and Blt of B. subtilis (21-24% identity), two members of the multidrug resistance efflux pump family. Our results indicate that we have identified a new locus which plays a crucial role in the cellobiose-dependent repression of hly expression.


Subject(s)
Bacterial Toxins , Cellobiose/pharmacology , Gene Expression Regulation, Bacterial , Genes, Bacterial , Heat-Shock Proteins/genetics , Listeria monocytogenes/genetics , Base Sequence , Cellobiose/metabolism , DNA Transposable Elements , DNA, Bacterial , Drug Resistance, Microbial/genetics , Drug Resistance, Multiple/genetics , Glucose/pharmacology , Heat-Shock Proteins/biosynthesis , Hemolysin Proteins , Listeria monocytogenes/metabolism , Molecular Sequence Data , Mutagenesis, Insertional , Open Reading Frames , Sequence Analysis, DNA , Transcription, Genetic
12.
Avian Pathol ; 28(2): 131-5, 1999 Apr.
Article in English | MEDLINE | ID: mdl-26911499

ABSTRACT

The heritability of resistance of poultry to Salmonella enteritidis (SE) was investigated. Three measurements of resistance were made: survival after intramuscular inoculation of 419 day-old chicks, absence versus presence of Salmonella in spleens and caeca 4 weeks after oral inoculation of 304 hens at peak of laying, and antibody response of 228 hens following two inoculations of an aroA mutant of this serotype. In the first two models of infection, resistance appeared to be heritable. The heritability was estimated from the sire and dam components, respectively, at 0.14 ± 0.10 and 0.62 ± 0.16 for chick mortality, 0.47 ± 0.21 and 0.13 ± 0.26 for resistance to spleen contamination, and 0.24 ± 0.15 and 0.53 ± 0.26 for resistance to caecal contamination in laying hens. By contrast the estimated heritability of antibody response was very low (0.03 ± 0.08 and 0.10 ± 0.08 when estimated from the sire and dam components, respectively). These results suggest that a selection for increased resistance to SE may be efficient.

13.
J Appl Microbiol ; 85(2): 337-46, 1998 Aug.
Article in English | MEDLINE | ID: mdl-9750307

ABSTRACT

Within the group of Listeria sp., only L. monocytogenes is pathogenic for humans and numerous studies of L. monocytogenes strains have described non-virulent isolates. In this study, the potential value of two tissue culture assays (TCA) was analysed to ascertain the virulence properties of L. monocytogenes strains, initially typed for virulence using the immunocompromised mouse model (ICMM). The first assay assessed both the penetration into, and multiplication within, Caco-2 cells (PM assay): the second was a plaque-forming assay (PF assay). All the clinical isolates (nine strains) were virulent in both TCA. Conversely, all the non-pathogenic species (seven strains) were non-virulent in PM and PF assays. Compared with the virulence obtained in the ICMM with 29 Listeria strains, including 12 non-virulent L. monocytogenes strains, the sensitivity of both TCA was equal to1. Specificity was 0.89 and 0.84 for the PF and PM assays, respectively. However, a study of strains exhibiting virulence differences in three other in vivo virulence models showed that ICMM only detected highly virulent strains. The specificity of the PF test could, therefore, be higher, and close to that obtained by the enumeration of viable bacteria in the spleen of mice infected by subcutaneous injection in the footpad and by intravenous injection. Taken together, this study confirms the existence of low-virulence L. monocytogenes strains and shows that the virulence status of some non-clinical L. monocytogenes isolates depends on the virulence models used. The data suggest that the PF assay could be used as a primary test to evaluate the virulence of Listeria strains in order to reduce the cost of testing all strains in vivo.


Subject(s)
Listeria monocytogenes/classification , Listeria monocytogenes/pathogenicity , Animals , Caco-2 Cells , Humans , Immunocompetence , Immunocompromised Host , Kinetics , Mice , Tumor Cells, Cultured , Virulence
14.
Poult Sci ; 77(6): 797-801, 1998 Jun.
Article in English | MEDLINE | ID: mdl-9628525

ABSTRACT

Previously, we have shown differences in susceptibility to the cecal carrier state in chicks orally infected with Salmonella enteritidis (SE) at 1 wk of age for four outbred lines: L2, B13, PA12, and Y11. The egg-type line L2 was one of the most susceptible lines and presented a large variability in cecal SE colonization. The heritability (h2) of the resistance to SE colonization in ceca was estimated in L2 chickens to determine whether genetic factors might be involved in its control. In three independent trials, a total of 819 L2 chicks produced from 88 sires and 232 dams were challenged orally with SE at 1 wk of age. Each week after inoculation, the frequency of cecal colonization was estimated. When this value had fallen to 50%, all the remaining animals were killed. The extent of cecal colonization by SE was estimated directly by counting the viable organisms in organs and determining the numbers of positive ceca. Enrichment culture was used in Trials 2 and 3. The effects of trial, of room within trial, and of cage within room on the frequency of SE contaminated ceca were often significant. No significant effect of sex was observed. Estimation of h2 using the frequency of SE positive ceca was low, 0.06 +/- 0.07, when results of direct culture were considered. In contrast, when considering the frequency obtained after enrichment, the h2 was estimated at 0.20 +/- 0.12. This result suggests a genetic basis for the expression of the resistance to colonization. An experiment of selection for resistance to SE carrier state in the chicken ceca should definitively confirm the genetic origin of the resistance.


Subject(s)
Carrier State/immunology , Cecum/microbiology , Salmonella Infections, Animal/immunology , Salmonella enteritidis , Animals , Chickens , Crosses, Genetic , Drug Resistance, Microbial , Female , Immunity, Innate/genetics , Male , Nalidixic Acid , Salmonella Infections, Animal/genetics , Salmonella enteritidis/genetics , Streptomycin
15.
Rev Sci Tech ; 16(2): 415-25, 1997 Aug.
Article in English | MEDLINE | ID: mdl-9501355

ABSTRACT

Foodborne diseases, in particular those related to meat and meat products, have recently become a matter of great public concern. Sheep and goat meat can transmit infections and diseases either through handling during preparation procedures or as a result of ingestion by the consumer. The authors highlight the second route of contamination in relation to meat and meat products from small ruminants in European countries. Among the most important diseases transmitted by mutton and goat meat, toxoplasmosis remains the greatest threat, particularly in immuno-compromised people and in pregnant women. Other pathogens which may be associated with the consumption of meat from small ruminants include: Clostridium perfringens, Cryptosporidium parvum and Campylobacter jejuni. As with other ruminant species, Escherichia coli O157:H7 can be considered as an emerging pathogen, for which control efforts must be made. The classical zoonoses (brucellosis, Q fever, hydatidosis) are also presented here, although the major source of contamination for these diseases remains contact with infected animals or the handling of carcasses. The fact that the association of foodborne diseases with mutton and goat meat is less frequent than with the meat of other animal species should be noted, for the following reasons: a) lower levels of production; b) less intensive production, leading to a weaker microbial contamination; c) mutton and goat meat are subjected to processing less often than other meats; d) the usual boiling or cooking processes.


Subject(s)
Bacterial Infections/transmission , Food Microbiology , Food Parasitology , Foodborne Diseases/epidemiology , Meat/microbiology , Parasitic Diseases/transmission , Toxoplasmosis/transmission , Animals , Europe/epidemiology , Female , Goats , Humans , Immunocompromised Host , Meat/parasitology , Pregnancy , Sheep
16.
Avian Dis ; 41(3): 559-67, 1997.
Article in English | MEDLINE | ID: mdl-9356700

ABSTRACT

Four chicken lines, L2, B13, PA12 (egg-type), and Y11 (meat-type), were tested for experimental carrier state of Salmonella enteritidis (SE) in two identical trials. After oral inoculation of SE at 1 wk of age with 5 x 10(4) SE colony-forming units (CFU), 10 chickens per line were necropsied weekly for 6 wk and then every 8 or 15 days until the 12th week postinoculation (PI). Liver, spleen, ovary, and ceca were examined for level of SE colonization. Numbers of positive livers and spleens and levels of the challenge strain in these organs differed little between the four chicken lines. Only three positive ovaries were detected. According to the chicken line, ceca exhibited generally significant (P < 0.05) differences in the number of positive organs during weeks 5-11 PI, in the SE CFU levels (P < 0.05) in the first 5 wk PI and during weeks 8 and 10 PI, and in the duration of colonization. L2 and B13 chickens generally carried SE in their ceca at higher levels, in more animals, and for a longer time than PA12 and Y11 chickens. Y11 chickens were the most resistant to SE cecal colonization.


Subject(s)
Carrier State/veterinary , Cecum/microbiology , Intestinal Mucosa/microbiology , Poultry Diseases , Salmonella Infections, Animal/transmission , Salmonella enteritidis , Animals , Carrier State/microbiology , Chickens , Female , Liver/microbiology , Ovary/microbiology , Salmonella Infections, Animal/physiopathology , Salmonella enteritidis/growth & development , Salmonella enteritidis/isolation & purification , Species Specificity , Spleen/microbiology , Time Factors
17.
Br Poult Sci ; 37(2): 329-39, 1996 May.
Article in English | MEDLINE | ID: mdl-8773842

ABSTRACT

1. Four groups of hens, each of a different line, were inoculated at peak of lay, per os in the crop with 1 ml of a suspension containing 10(9) cfu/ml Salmonella enteritidis PT4 (SE). The kinetics of SE contamination in the environment, egg shell and yolk were studied during the first 28 d post inoculation. On the day of slaughter, intestines, caeca, spleen, liver, ovary, oviduct and content were investigated for SE contamination. 2. The commercial egg-type line L2 was found to be the most susceptible to SE. It laid many SE-positive yolks (13.8%) and internal and faecal organs were frequently infected. 3. Certain lines are found to exhibit a degree of resistance to SE; the cause of which is unknown and might be attributed to major genes.


Subject(s)
Salmonella Infections, Animal/immunology , Salmonella enteritidis , Abattoirs , Animals , Chickens , Disease Susceptibility , Egg Shell/microbiology , Egg Yolk/microbiology , Female , Humans , Immunity, Innate , Intestines/microbiology , Kinetics , Liver/microbiology , Ovary/microbiology , Oviducts/microbiology , Salmonella Infections/prevention & control , Salmonella enteritidis/isolation & purification , Spleen/microbiology
18.
FEMS Microbiol Lett ; 135(2-3): 161-7, 1996 Jan 15.
Article in English | MEDLINE | ID: mdl-8595853

ABSTRACT

Salmonella serotype typhimurium transpositional mutants altered in resistance to biliary salts and detergents were isolated previously. We have characterized further the LX1054 mutant strain, the most sensitive of them. The chromosomal DNA segment flanking transposon insertion was cloned and sequenced. The highest level of identity was found for the acrB (formerly acrE) gene of Escherichia coli, a gene encoding a drug efflux pump of the Acr family. LX1054 exhibited a reduced capacity to colonize the intestinal tract. After passages in mice, the mutant strain lost the sensitive phenotype. In vitro, a resumption of growth appeared after 17 h of culture in medium with cholate or other tested biological or chemical detergents. Then, the acquired resistant phenotype seemed stable. The data suggested a role of S. typhimurium acrB-like gene in resistance to biliary salts and detergents and in mice intestinal colonization. However, the local and transient sensitivity observed in vivo, and the in vitro adaptations suggest that several detergent-resistance mechanisms operate in S. typhimurium.


Subject(s)
Bacterial Proteins/genetics , Carrier Proteins , Cholic Acids/pharmacology , Escherichia coli Proteins , Genes, Bacterial , Membrane Proteins/genetics , Salmonella Infections, Animal/microbiology , Salmonella typhimurium/physiology , Amino Acid Sequence , Animals , Bacterial Proteins/physiology , Base Sequence , Cloning, Molecular , Drug Resistance, Microbial , Female , Intestines/microbiology , Membrane Proteins/physiology , Mice , Mice, Inbred C57BL , Mice, Mutant Strains , Microbial Sensitivity Tests , Molecular Sequence Data , Multidrug Resistance-Associated Proteins , Mutation , Salmonella typhimurium/drug effects , Salmonella typhimurium/genetics , Salmonella typhimurium/pathogenicity , Sequence Analysis, DNA , Sodium Dodecyl Sulfate/pharmacology
19.
Res Microbiol ; 146(8): 659-70, 1995 Oct.
Article in English | MEDLINE | ID: mdl-8584789

ABSTRACT

Salmonella typhimurium is a ubiquitous pathogenic bacterium able to sustain the environmental conditions of the gastrointestinal tract, including biliary salts. To understand the mechanisms involved in bile salt resistance and, more generally, detergent resistance, we investigated S. typhimurium mutants produced with the random mutagenic TnphoA transposon. A total of 3,000 transpositional mutants were isolated. Three strains among the 1,432 first mutants lost the ability to grow in the presence of biological and chemical detergents. They were prototrophic and exhibited normal lipopolysaccharide and outer membrane protein profiles after SDS-PAGE. They did not show sensitivity to dyes but showed very different sensitivities to antibiotics. For each mutant strain, Southern blotting analysis revealed a unique TnphoA insertion at different chromosomal locations. These observations were confirmed by transduction experiments.


Subject(s)
Anti-Bacterial Agents/pharmacology , Bile Acids and Salts/pharmacology , DNA Transposable Elements/genetics , Detergents/pharmacology , Salmonella typhimurium/drug effects , Bacterial Outer Membrane Proteins/analysis , Blotting, Southern , DNA, Bacterial/chemistry , Drug Resistance, Microbial , In Vitro Techniques , Lipopolysaccharides/biosynthesis , Mutagenesis, Insertional , Salmonella typhimurium/genetics , Salmonella typhimurium/growth & development , Salmonella typhimurium/metabolism
20.
Avian Dis ; 39(4): 796-803, 1995.
Article in English | MEDLINE | ID: mdl-8719213

ABSTRACT

Quantification of the carrier state of Salmonella enteritidis in chicks (i.e., persistent asymptomatic association of S. enteritidis with the host), should provide an optimized means for further investigations into this problem. We therefore developed an experimental carrier state model by oral inoculation of low doses (10(2)-10(4)) of S. enteritidis in B13 chicks at different ages. Liver, spleen, and ceca colonizations by the challenge strains were measured weekly by enumeration of S. enteritidis colony-forming units (CFU) for 7-12 weeks. High mortality rates, incompatible with the carrier state, were observed in chicks inoculated with 10(2) organisms of either a parental strain of S. enteritidis (5556) or a mutant resistant to streptomycin (Smr) and nalidixic acid (Nalr) (strain 1009) at 1 day old. Both strains colonized organs similarly, allowing us to use subsequently the SmrNalr mutant strain. The selected low doses of S. enteritidis induced no deaths in chicks inoculated at 1 or 3 weeks of age. However, inoculation of 3-week-old chicks did not induce a satisfactory carrier state; organ colonization by S. enteritidis was weak and transient, even after inoculation of 10(8) SE. In contrast, some birds infected at 1 week of age presented the challenge strain in the liver and spleen for 3 weeks after inoculation and in the ceca for 12 weeks postchallenge. Most of these birds were colonized by S. enteritidis in the liver and in the ceca for 3 weeks and 10 weeks, respectively, following inoculation. Generally, CFU levels were highest during the first week(s) after inoculation and then decreased progressively. Levels of S. enteritidis were lower in the liver and spleen than in the ceca. Oral inoculation of 1-week-old birds with 5 x 10(4) S. enteritidis provided the required model, allowing quantification of the carrier state of S. enteritidis in chicks.


Subject(s)
Carrier State/veterinary , Poultry Diseases , Salmonella Infections, Animal/diagnosis , Salmonella enteritidis , Animals , Carrier State/diagnosis , Carrier State/immunology , Chi-Square Distribution , Chickens , Cloaca/microbiology , Immunity, Innate , Liver/microbiology , Salmonella Infections, Animal/immunology , Salmonella enteritidis/isolation & purification , Species Specificity , Spleen/microbiology , Time Factors
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