ABSTRACT
BACKGROUND: Caries is a public health problem, given that it prevails in 60 to 90% of the school-age global population. Multiple factors interact in its etiology, among them dental plaque is necessary to have lactic acid producing microorganisms like Streptococcus from he Mutans group. Existing prevention and treatment measures are not totally effective and generate adverse effects, which is why it is necessary to search for complementary strategies for their management. AIM: The study sought to evaluate the eradication capacity of Streptococcus mutans biofilms and the toxicity on eukaryotic cells of Lippia alba and Cymbopogon citratus essential oils. METHODOLOGY: Essential oils were extracted from plant material through steam distillation and then its chemical composition was determined. The MBEC-high-throughput (MBEC-HTP) (Innovotech, Edmonton, Alberta, Canada) assay used to determine the eradication concentration of S. mutans ATCC 35668 strain biofilms. Cytotoxicity was evaluated on CHO cells through the MTT cell proliferation assay. RESULTS: The major components in both oils were Geraniol and Citral; in L. alba 18.9% and 15.9%, respectively, and in C. citratus 31.3% and 26.7%. The L. alba essential oils presented eradication activity against S. mutans biofilms of 95.8% in 0.01mg/dL concentration and C. citratus essential oils showed said eradication activity of 95.4% at 0.1, 0.01mg/dL concentrations and of 93.1% in the 0.001mg/dL concentration; none of the concentrations of both essential oils showed toxicity on CHO cells during 24h. CONCLUSION: The L. alba and C. citratus essential oils showed eradication activity against S. mutans biofilms and null cytotoxicity, evidencing the need to conduct further studies that can identify their active components and in order to guide a safe use in treating and preventing dental caries.
Subject(s)
Biofilms/drug effects , Cymbopogon/chemistry , Lippia/chemistry , Oils, Volatile/pharmacology , Plant Oils/pharmacology , Plant Preparations/pharmacology , Acyclic Monoterpenes , Animals , CHO Cells , Cell Line , Cricetulus , Dental Caries/drug therapy , Dental Caries/microbiology , Microbial Sensitivity Tests/methods , Monoterpenes/chemistry , Monoterpenes/pharmacology , Oils, Volatile/chemistry , Plant Oils/chemistry , Plant Preparations/chemistry , Streptococcus mutans/drug effects , Terpenes/chemistry , Terpenes/pharmacologyABSTRACT
La degeneración de las articulaciones axiales y periféricas va unida al propio envejecimiento del ser humano. Es responsable del dolor e impotencia funcional, y su fisiopatología es compleja y no del todo conocida. El denominador común en la artrosis es la degeneración del cartílago articular, junto a otras alteraciones secundarias. Su abordaje y manejo del dolor supone todo un reto para las Unidades del Dolor. Historicamente, el tratamiento intervencionista se llevo a cabo mediante infiltraciones a ciegas de diferentes sustancias que alivian el dolor. En la actualidad, la fluoroscopia, y más recientemente los ultrasonidos, son los métodos de elección para la guía de nuestros procedimientos. En cuanto a los fármacos utilizados más comunmente en nuestras técnicas invasivas articulares destacaremos el ácido hialurónico (AH) y el plasma rico en plaquetas (PRP). Los esteroides siguen siendo, en prácticamente todos los estudios, el estándar de oro o fármaco de referenda y sobre los que se comparan el resto de familias de fármacos. Una exploración exhaustiva del paciente, y un algoritmo de decisiones en cuanto a la decisión de realización de estas técnicas, se antoja necesario, adecuandose a cada lugar de trabajo (AU)
The degeneration of the axial and peripheral joints are linked to the aging of the human being. It is responsible for local pain, and its pathophysiology is complex and not fully understood. The common denominator in osteoarthritis is the degeneration of articular cartilage, along with other secondary changes. His approach and pain management is a challenge for pain units. Historically the interventional treatment was carried out by blind injections of different substances that relieve pain. Currently, fluoroscopy, and more recently ultrasound, are the methods of choice for the guidance of our procedures. Regarding the drugs most commonly used in our joint invasive techniques we highlight hyaluronic acid (HA) and platelet rich plasma (PRP). Steroids remain in almost all studies the gold standard or reference drug, and on the other families of drugs are compared. A thorough examination of the patient, and an algorithm of decisions regarding the decision to carry out these techniques seems necessary, adapting to each workplace (AU)
Subject(s)
Humans , Male , Female , Osteoarthritis/drug therapy , Hyaluronic Acid/therapeutic use , Platelet-Rich Plasma , Pulsed Radiofrequency Treatment , Adrenal Cortex Hormones/therapeutic use , Osteoarthritis/complications , Quality of Life , Algorithms , Knee Joint , Osteoarthritis, Knee/drug therapy , Osteoarthritis, KneeABSTRACT
No disponible
Subject(s)
Humans , Male , Female , Pain Clinics , Pain Clinics/organization & administration , Nursing Assessment/ethics , Nursing Assessment/methods , Physician Assistants/education , Physician Assistants/standards , Patient Care Team/standards , Public Health/ethics , Public Health/methods , Pain Clinics/standards , Pain Clinics , Nursing Assessment/standards , Nursing Assessment , Physician Assistants/psychology , Physician Assistants , Patient Care Team/classification , Public Health , Public Health/standardsABSTRACT
El hombro doloroso es una entidad que encontramos en nuestra práctica diaria con relativa frecuencia. Con la introducción de la ultrasonografía, se abre un campo novedoso ya que podemos explorar, localizar e infiltrar exactamente la zona lesionada. La ecografía permite una exploración en la misma consulta, así como la capacidad de realizar una exploración dinámica. El hombro se compone de 5 articulaciones: 3 verdaderas (esternoclavicular, acromioclavicular y glenohumeral) y 2 denominadas falsas (subacromial y escapulotorácica). Siempre que sea posible, intentaremos realizar la punción en plano, intentando visualizar el trayecto completo de la aguja y la punta de la misma. En el intervencionismo del hombro esto es bastante fácil de conseguir puesto que se trata de estructuras muy superficiales. Es muy poco probable que se produzcan daños graves durante la infiltración del hombro. Tendremos especial cuidado de no puncionar estructuras vasculares (AU)
Painful shoulder is an entity that we find in our daily practice with relative frequency. With the introduction of ultrasound, opens a new field that we can explore, locate and exactly infiltrate the injured area. Ultrasound allows an exploration in the same act, as well as the ability to make a dynamic scanning. The shoulder is made up of 5 joint: true 3 (sternoclavicular, acromioclavicular and glenohumeral) and 2 known as false (subacromial and thoracic scapula). Wherever possible, we will try to puncture in plane, trying to display the full path of the needle and the tip of it. In the interventionism of the shoulder, this is quite easy to get since its very superficial structures. It is highly unlikely any serious damage during the infiltration of the shoulder. Take special care of non piercing vascular structures (AU)
Subject(s)
Humans , Male , Female , Shoulder Joint , Shoulder Pain/therapy , Shoulder Pain , Shoulder/pathology , Shoulder , Tendons/pathology , Tendons , Pulsed Radiofrequency Treatment/trends , Pulsed Radiofrequency TreatmentABSTRACT
The percutaneous, intraluminal placement of an expandable arterial stent is a new method for reducing stenosis in atherosclerotic arteries. This method, used in conjunction with balloon angioplasty, involves the placement of metallic mesh stent at the sight of the stenosis. Use of this technique has resulted in various improvements over angioplasty alone.
