Segmented flow generator for serial crystallography at the European X-ray free electron laser.

Serial femtosecond crystallography (SFX) with X-ray free electron lasers (XFELs) allows structure determination of membrane proteins and time-resolved crystallography. Common liquid sample delivery continuously jets the protein crystal suspension into the path of the XFEL, wasting a vast amount of sample due to the pulsed nature of all current XFEL sources. The European XFEL (EuXFEL) delivers femtosecond (fs) X-ray pulses in trains spaced 100 ms apart whereas pulses within trains are currently separated by 889 ns. Therefore, continuous sample delivery via fast jets wastes >99% of sample. Here, we introduce a microfluidic device delivering crystal laden droplets segmented with an immiscible oil reducing sample waste and demonstrate droplet injection at the EuXFEL compatible with high pressure liquid delivery of an SFX experiment. While achieving ~60% reduction in sample waste, we determine the structure of the enzyme 3-deoxy-D-manno-octulosonate-8-phosphate synthase from microcrystals delivered in droplets revealing distinct structural features not previously reported.

Implant Rehabilitation According to the Biologically Oriented Preparation Technique (BOPT): A Medium-Term Retrospective Study.

Clinical records of patients who underwent implant-supported rehabilitation according to the biologically oriented preparation technique (BOPT) principles were retrospectively analyzed. Records of 189 nonconsecutive patients who received 502 implants were reviewed. At the last follow-up visit (occurring on average 5.11 years after prosthesis delivery), 466 (92.8%) implants had a Gingival Index of 0, and 491 (97.8%) presented no bleeding on probing. Four hundred eighty-nine crowns on as many implants (97.4%) showed no sign of gingival recession. Technical complications occurred with 10 implants (2.0%) and 6 patients (3.2%). Biologic complications were detected with 14 implants (2.8%) and 6 patients (3.2%). When the BOPT approach is applied to rehabilitate patients using implant-supported prostheses, excellent medium-term results concerning soft tissue health may be achieved.

Prevalence of Peri-Implantitis: A Multi-Centered Cross-Sectional Study on 248 Patients.

The aim of this multicenter cross-sectional study was to determine the prevalence of peri-implantitis and to assess its association with several patient- and implant-related factors. Patients with at least one implant, who came for a recall visit to one of the four centers over a period of five months, were enrolled. Presence of peri-implantitis (defined as bleeding on probing, exudate/suppuration, bone loss > 0.2 mm/year and increased pocket depth) and several other variables (e.g., smoking habits, history of periodontitis, diabetes) were recorded. Out of 248 enrolled patients (1162 implants), 10 patients had at least one implant with peri-implantitis (4.03%); a total of 14 implants were affected (1.20%). A statistically significant association between peri-implantitis and diabetes was found (OR 8.65; CI: 1.94-38.57). Smoking more than 10 cigarettes per day (OR: 0.53; CI 0.03-9.45) and history of periodontitis (OR: 2.42; CI: 0.49-11.89) were not found to be statistically associated with peri-implantitis. Even if implant therapy is a consolidated treatment, biological complications do happen. Strict supportive therapy recalls could lead to lower rates of peri-implantitis and earlier diagnosis.

The Karabo distributed control system.

The Karabo distributed control system has been developed to address the challenging requirements of the European X-ray Free Electron Laser facility, including complex and custom-made hardware, high data rates and volumes, and close integration of data analysis for distributed processing and rapid feedback. Karabo is a pluggable, distributed application management system forming a supervisory control and data acquisition environment as part of a distributed control system. Karabo provides integrated control of hardware, monitoring, data acquisition and data analysis on distributed hardware, allowing rapid control feedback based on complex algorithms. Services exist for access control, data logging, configuration management and situational awareness through alarm indicators. The flexible framework enables quick response to the changing requirements in control and analysis, and provides an efficient environment for development, and a single interface to make all changes immediately available to operators and experimentalists.

Giant reed genotypes from temperate and arid environments show different response mechanisms to drought.

Studies at the root level and how the root-shoot interactions may influence the whole crop performance of giant reed (Arundo donax L.) under limited water conditions are largely missing. In the present study, we illustrate the effects of water stress on some phenotypic traits at the root-shoot levels of two giant reed genotypes (from Morocco and Northern Italy) that were reported to have different adaptive hydraulic stem conductivities despite the limited genetic variability of the species. The trial was carried out in 1 m3 rhizotrons (1 × 1 × 1 m) for two consecutive growing seasons. As expected, both genotypes showed an effective behavior to contrast water shortage; however, the Moroccan genotype showed a higher leaf water potential, a lower root length density (RLD) and thinner roots in the upper soil layer (0-20 cm), and similar to control RLD values at deep soil layers (40-60 cm). On the other hand the Italian genotype showed the opposite pattern; that is no drought (DR) effects in RLD and root diameter at upper soil layers and reduced RLD in deep layers, thus revealing different DR adaptation characteristics between two genotypes. This DR adaptation variability might bring new insights on DR tolerance of giant reed identifying potential traits aimed to improve the integral plant functioning, to a more efficient use of water resources, and to a more effective crop allocation to targeted stressful conditions under a climate change scenario that foresees the increase of DR periods.

Assessment of Long-Term Survival of Immediately Loaded Tilted Implants Supporting a Maxillary Full-Arch Fixed Prosthesis.

PURPOSE: To analyze the long-term outcome of fixed prostheses supported by six implants, two of which were tilted, placed in the maxilla and immediately loaded more than 10 years earlier. MATERIALS AND METHODS: A retrospective review of implants placed between May 29, 2003 and February 12, 2005 and used to support immediately loaded fixed dental prostheses in the maxilla was conducted. The features of failed implants were analyzed. In the most recent follow-up visits, survival of individual implants and prostheses was verified, and modified Plaque Index as well as modified Sulcular Bleeding Index were assessed. Patients also filled out a questionnaire requiring graded responses from 0 (poor) to 10 (excellent) that was designed to assess their quality of life. RESULTS: A total of 162 implants were placed between May 29, 2003 and February 12, 2005 to support immediately loaded maxillary fixed prostheses of 27 totally edentulous patients (19 female, 8 male). Three patients (1 male, 2 female) dropped out, so 144 implants were followed up. Seven of the 144 original implants failed, corresponding to a survival rate of 95.1% over 10 years. All the failures occurred within 2 years after surgery. Patients' responses to the questionnaire produced an average score of 8.4 to 8.8, showing a relevant degree of satisfaction. CONCLUSION: Based upon this study of 27 patients who received immediately loaded maxillary full-arch fixed implant-supported prostheses supported by two tilted and four axial implants, it appears that this is a reliable procedure with a high long-term survival rate and a high level of patient satisfaction.

Evaluation of accuracy and precision of a new guided surgery system: a multicenter clinical study.

Computer-aided design/computer-assisted manufacture (CAD/CAM) guides for surgery are becoming a widespread tool in implant dentistry. This study sought to evaluate the accuracy and precision of a new guided surgery system. Twenty-five patients were treated in eight centers, and a total of 117 implants were placed using CAD/CAM surgical guides supported by bone, mucosa, and/or teeth. A postoperative computed tomographic (CT) scan of each patient was taken and superimposed on a preoperative CT scan to evaluate any discrepancies between the planned and actual implant positions (apex and platform positions), as well as the implant tilt. Implant placement using bone- and mucosa-supported guides was found to be more precise compared to using guides supported by teeth or a combination of teeth and mucosa. However, the differences were not statistically significant. The accuracy of the guided surgery system is in line with the data found in the literature. Considering the mean positioning discrepancies between the planned and actual implant outcomes, clinicians are advised to maintain a safe distance between implants and anatomical structures of at least 2 mm. In immediate loading cases, relining a provisional prosthesis to compensate for any discrepancies between the virtual and clinical implant positions is recommended.

Prevention and treatment of postoperative infections after sinus elevation surgery: clinical consensus and recommendations.

Introduction. Maxillary sinus surgery is a reliable and predictable treatment option for the prosthetic rehabilitation of the atrophic maxilla. Nevertheless, these interventions are not riskless of postoperative complications with respect to implant positioning in pristine bone. Aim. The aim of this paper is to report the results of a clinical consensus of experts (periodontists, implantologists, maxillofacial surgeons, ENT, and microbiology specialists) on several clinical questions and to give clinical recommendations on how to prevent, diagnose, and treat postoperative infections. Materials and Methods. A panel of experts in different fields of dentistry and medicine, after having reviewed the available literature on the topic and taking into account their long-standing clinical experience, gave their response to a series of clinical questions and reached a consensus. Results and Conclusion. The incidence of postop infections is relatively low (2%-5.6%). A multidisciplinary approach is advisable. A list of clinical recommendation are given.

Multicenter retrospective analysis of 201 consecutively placed camlog dental implants.

Camlog tube-in-tube implants were introduced several years ago. This multicenter retrospective analysis evaluated the success rate of Root-Line and Screw-Line tube-in-tube implants in daily use. A total of 201 implants were placed in 96 patients with different indications and implantation procedures. Implants were analyzed retrospectively after a functional loading period of 12 to 78 months. At the end of the observation period, all but 1 implant fulfilled the success criteria, resulting in an implant survival rate of 99.5%. Individual case analysis of implants in special indications, such as immediate loading, short implants, and tilted implants, did not indicate any increased risk of implant failure.

Hyaluronic acid to improve healing of surgical incisions in the oral cavity: a pilot multicentre placebo-controlled randomised clinical trial.

PURPOSE: To evaluate the efficacy of hyaluronic acid to improve the healing of surgical incisions in the oral cavity. MATERIALS AND METHODS: Six Italian private practices participated in this trial, each centre provided 12 patients. After suturing, patients were randomised to receive either a single application of 0.8% hyaluronic acid or a placebo (the carrier). Outcome measures were: assessment of wound healing 10 days post-operatively on a Likert scale by the blind operators and by an independent and blinded outcome assessor on the photographs, adverse events and post-operative complications. Reproducibility was assessed by evaluating agreement between operators and the independent outcome assessor using the weighted Kappa statistic. RESULTS: Thirty-six patients were evaluated in each group, at ten days none had dropped-out. No post-operative complications or adverse events occurred. There were no statistically significant differences for wound healing, assessed clinically by the blinded operators or on photographs evaluated by a blinded and independent outcomes assessor. There was a substantial agreement between operators and the independent outcome assessor in the wound scoring. CONCLUSIONS: Hyaluronic acid placed over surgical incisions in the oral cavity does not appear to improve wound healing. Further trials are needed to better understand the potential role of hyalulonic acid in dental applications.