Subject(s)
Coronary Disease/etiology , Hyperlipoproteinemia Type II/enzymology , Pancreatic Elastase/blood , alpha 1-Antitrypsin Deficiency , Adolescent , Adult , Coronary Disease/enzymology , Coronary Disease/genetics , Enzyme Activation , Female , Humans , Hyperlipoproteinemia Type II/complications , Male , Middle Aged , Pancreatic Elastase/genetics , Sex Factors , alpha 1-Antitrypsin/geneticsSubject(s)
Aortitis/diagnosis , Arteritis/diagnosis , Clinical Enzyme Tests , Pancreatic Elastase/blood , Adolescent , Adult , Blood Sedimentation , Female , Humans , MaleABSTRACT
The data obtained allow to consider urine acid-stable proteinase inhibitor (ASPI) activity as an early and informative index of renal parenchyma damage in patients with renal symptomatic arterial hypertensions without significant urine changes. Urine ASPI activities seems to be useful in differentiation of patients with chronic glomerulonephritis without significant urine changes and patients with hypertensions.
Subject(s)
Chymotrypsin/blood , Glycoproteins/urine , Hypertension, Renal/metabolism , Pancreatic Elastase/blood , Adolescent , Adult , Child , Female , Humans , Hypertension, Renal/etiology , Kidney Diseases/complications , Male , Middle Aged , Toxins, Biological/bloodSubject(s)
Peptides/metabolism , Toxemia/diagnosis , Anemia/etiology , Anemia/metabolism , Animals , Hemoperfusion , Humans , Kidney Failure, Chronic/diagnosis , Kidney Failure, Chronic/metabolism , Kidney Failure, Chronic/therapy , Molecular Weight , Peptides/analysis , Peptides/toxicity , Toxemia/metabolism , Toxemia/therapy , Toxins, Biological/analysis , Toxins, Biological/metabolism , Uremia/diagnosis , Uremia/metabolismABSTRACT
The method for the preparation of a specific proteinase adsorbent (acid-resistant human urinary proteinase inhibitor--UPI--immobilized on sepharose) has been developed. Proteinase adsorption using this adsorbent has proved highly effective in the treatment of acute pancreatitis with plasmasorption. High adsorption capacity of UPI-sepharose from plasma samples of patients with acute nonspecific aortoarteritis has been demonstrated in vitro. Determination of UPI activity in the human urine is an informative test for early tissue damage, as compared to plasma middle molecules assay. The former test may be used as a diagnostic technique in patients with parenchymal kidney injury and increased arterial pressure.
Subject(s)
Protease Inhibitors/therapeutic use , Acute Disease , Adult , Animals , Aortitis/enzymology , Aortitis/therapy , Arteritis/enzymology , Arteritis/therapy , Dogs , Drug Evaluation , Drug Evaluation, Preclinical , Drug Stability , Female , Hemoperfusion/methods , Humans , Pancreas/enzymology , Pancreatitis/enzymology , Pancreatitis/therapy , Peptide Hydrolases/blood , Protease Inhibitors/isolation & purification , Protease Inhibitors/urine , Sepharose , Takayasu Arteritis/enzymology , Takayasu Arteritis/therapyABSTRACT
The determination of urine acid-stable proteinase inhibitor activity proved to be more informative at the early stages of renal tissue lesions, as compared to measurement of intermediate molecule blood level. It can be used as a diagnostic test in patients with parenchymatous renal diseases accompanied by blood pressure elevation, and essential hypertension.