ABSTRACT
Enzyme linked immunosorbent assay (ELISA) was used for the detection of ABH blood group substances in body fluids. The original procedure was modified to provide maximum sensitivity and reliability. The modified ELISA method proved to be more sensitive and required much less sample than previously described techniques.
Subject(s)
ABO Blood-Group System/analysis , Enzyme-Linked Immunosorbent Assay , Saliva/chemistry , Semen/chemistry , Vagina/chemistry , Female , Humans , Male , Predictive Value of TestsABSTRACT
It was observed during the course of routine casework that different bloodstains from the same individual could produce anomalies in the glyoxalase I band patterns. Bloodstains were heated at different temperatures for periods of 4 and 6 h and then examined using electrophoretic techniques. It was demonstrated that upon heating, band alterations in the glyoxalase I Type 1 phenotype can occur, causing the analyst to render the results inconclusive.
