ABSTRACT
Antimicrobial peptide loaded poly(2-hydroxyethyl methacrylate) particles were synthesized in supercritical carbon dioxide via one-pot free-radical dispersion polymerisation of 2-hydroxyethyl methacrylate and a cross-linker. Discrete particles with a well-defined spherical morphology and a diameter as low as 450â¯nm have been obtained in mild conditions. The encapsulation and release of the peptide were confirmed by antimicrobial tests that demonstrated for the first time a sustained release of the peptide from poly(2-hydroxyethyl methacrylate) microgels prepared by one-pot dispersion polymerization in supercritical carbon dioxide and then dispersed in water.
Subject(s)
Anti-Bacterial Agents/chemistry , Antimicrobial Cationic Peptides/chemistry , Drug Carriers/chemistry , Nanoparticles/chemistry , Anti-Bacterial Agents/pharmacology , Antimicrobial Cationic Peptides/pharmacology , Bradykinin/chemistry , Bradykinin/pharmacology , Carbon Dioxide/chemistry , Cross-Linking Reagents/chemistry , Drug Liberation , Drug Resistance, Bacterial , Humans , Microbial Sensitivity Tests , Particle Size , Peptides/chemistry , Peptides/pharmacology , Polyhydroxyethyl Methacrylate/chemistry , Polymerization , Staphylococcus aureus/drug effects , Surface PropertiesABSTRACT
Sulindac loaded poly(HEMA) cross-linked microparticles were synthesized via one-pot free-radical dispersion polymerisation in supercritical carbon dioxide (scCO2) in presence of photocleavable diblock stabilisers based on polyethylene oxide (PEO) and poly(heptadecafluorodecyl acrylate) (PFDA) bearing a o-nitrobenzyl photosensitive junction (hv) (PEO-hv-PFDA), and ethylene glycol dimethacrylate (EGDMA) as cross-linker. Poly(HEMA) cross-linked microparticles either empty or sulindac loaded were obtained with well-defined spherical morphology with the sizes between 250 and 350â¯nm. Additionally, upon UV-photolysis the stabiliser on the surface was cleaved which permits to microparticles to be redispersed in water leading to water swollen microgels about 2.1-3.6⯵m. Moreover, the release behaviour from obtained microgels indicated the sustained release of sulindac over 10â¯days. Besides, the surface modification after UV-photolysis was studied and proved that the particles can be functionalised with further chemistries.
