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J Microbiol Biotechnol ; 27(7): 1331-1335, 2017 Jul 28.
Article in English | MEDLINE | ID: mdl-28478659

ABSTRACT

In this study, the feasibility of introducing calcite-forming bacteria into concrete pavements to improve their mechanical performance was investigated. Lysinibacillus sphaericus WJ-8, which was isolated in a previous study and is capable of exhibiting high urease activity and calcite production, was used. When analyzed via scanning electron microscopy (SEM) and X-ray diffraction, WJ-8 showed a significant amount of calcite precipitation. The compressive strength of cement mortar mixed with WJ-8 cells and nutrient medium (urea with calcium lactate) increased by 10% compared with that of the controls. Energy dispersive x-ray spectroscopy analyses confirmed that the increase in strength was due to the calcite formed by the WJ-8 cells.


Subject(s)
Bacillaceae/metabolism , Bacterial Physiological Phenomena , Calcium Carbonate/chemistry , Chemical Precipitation , Construction Materials/microbiology , Bacillaceae/enzymology , Bacillaceae/growth & development , Calcium Compounds , Compressive Strength , Lactates , Microscopy, Electron, Scanning , Spectrometry, X-Ray Emission , Urea/metabolism , Urease/biosynthesis , X-Ray Diffraction
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