Impact of the Oral Administration of Polystyrene Microplastics on Hepatic Lipid, Glucose, and Amino Acid Metabolism in C57BL/6Korl and C57BL/6-Lepem1hwl/Korl Mice.

The impact of microplastics (MPs) on the metabolic functions of the liver is currently unclear and not completely understood. To investigate the effects of the administration of MPs on the hepatic metabolism of normal and obese mice, alterations in the lipid, glucose (Glu), and amino acid regulation pathways were analyzed in the liver and adipose tissues of C57BL/6Korl (wild type, WT) or C57BL/6-Lepem1hwl/Korl mice (leptin knockout, Lep KO) orally administered polystyrene (PS) MPs for 9 weeks. Significant alterations in the lipid accumulation, adipogenesis, lipogenesis, and lipolysis pathways were detected in the liver tissue of MP-treated WT and Lep KO mice compared to the vehicle-treated group. These alterations in their liver tissues were accompanied by an upregulation of the serum lipid profile, as well as alterations in the adipogenesis, lipogenesis, and lipolysis pathways in the adipose tissues of MP-treated WT and Lep KO mice. Specifically, the level of leptin was increased in the adipose tissues of MP-treated WT mice without any change in their food intake. Also, MP-induced disruptions in the glycogenolysis, Glu transporter type 4 (GLUT4)-5' AMP-activated protein kinase (AMPK) signaling pathway, levels of lipid intermediates, and the insulin resistance of the liver tissues of WT and Lep KO mice were observed. Furthermore, the levels of seven endogenous metabolites were remarkably changed in the serum of WT and Lep KO mice after MP administrations. Finally, the impact of the MP administration observed in both types of mice was further verified in differentiated 3T3-L1 adipocytes and HepG2 cells. Thus, these results suggest that the oral administration of MPs for 9 weeks may be associated with the disruption of lipid, Glu, and amino acid metabolism in the liver tissue of obese WT and Lep KO mice.

Identification of an Antagonist Targeting G Protein and ß-Arrestin Signaling Pathways of 5-HT7R.

In consideration of the limited number of FDA-approved drugs for autism spectrum disorder (ASD), significant efforts have been devoted to identifying novel drug candidates. Among these, 5-HT7R modulators have garnered considerable attention due to their potential in alleviating autism-like behaviors in ASD animal models. In this study, we designed and synthesized biphenyl-3-ylmethylpyrrolidines 3 and biphenyl-3-yl-dihydroimidazoles 4 as 5-HT7R modulators. Through extensive biological tests of 3 and 4 in G protein and ß-arrestin signaling pathways of 5-HT7R, it was determined that 2-(2'-methoxy-[1,1'-biphenyl]-3-yl)-4,5-dihydro-1H-imidazole 4h acted as a 5-HT7R antagonist in both signaling pathways. In in vivo study with Shank3-/- transgenic (TG) mice, the self-grooming behavior test was performed with 4h, resulting in a significant reduction in the duration of self-grooming. In addition, an immunohistochemical experiment with 4h restored reduced neurogenesis in Shank3-/- TG mice, which is confirmed by the quantification of doublecortin (DCX) positive neurons, suggesting the promising therapeutic potential of 4h.

Complement C3-Deficiency-Induced Constipation in FVB/N-C3em1Hlee/Korl Knockout Mice Was Significantly Relieved by Uridine and Liriope platyphylla L. Extracts.

Complement component 3 (C3) deficiency has recently been known as a cause of constipation, without studies on the therapeutic efficacy. To evaluate the therapeutic agents against C3-deficiency-induced constipation, improvements in the constipation-related parameters and the associated molecular mechanisms were examined in FVB/N-C3em1Hlee/Korl knockout (C3 KO) mice treated with uridine (Urd) and the aqueous extract of Liriope platyphylla L. (AEtLP) with laxative activity. The stool parameters and gastrointestinal (GI) transit were increased in Urd- and AEtLP-treated C3 KO mice compared with the vehicle (Veh)-treated C3 KO mice. Urd and AEtLP treatment improved the histological structure, junctional complexes of the intestinal epithelial barrier (IEB), mucin secretion ability, and water retention capacity. Also, an improvement in the composition of neuronal cells, the regulation of excitatory function mediated via the 5-hydroxytryptamine (5-HT) receptors and muscarinic acetylcholine receptors (mAChRs), and the regulation of the inhibitory function mediated via the neuronal nitric oxide synthase (nNOS) and inducible NOS (iNOS) were detected in the enteric nervous system (ENS) of Urd- and AEtLP-treated C3 KO mice. Therefore, the results of the present study suggest that C3-deficiency-induced constipation can improve with treatment with Urd and AEtLP via the regulation of the mucin secretion ability, water retention capacity, and ENS function.

Effect of Gate Bias Stress on the Electrical Characteristics of Ferroelectric Oxide Thin-Film Transistors with Poly(Vinylidenefluoride-Trifluoroethylene).

We investigated the effect of gate bias stress (GBS) on the electrical characteristics of ferroelectric oxide thin-film transistors (FeOxTFTs) with poly(vinylidenefluoride-trifluoroethylene). Generally, conventional oxide thin-film transistors (OxTFTs) with dielectric gate insulators exhibit a small negative shift under negative gate bias stress (NBS) and a large positive shift under positive gate bias stress (PBS) in transfer characteristic curves. In contrast, the FeOxTFTs show a small positive shift and a large negative shift under NBS and PBS, respectively. It was confirmed that sufficient changes in the electrical characteristics are obtained by 10 min NBS and PBS. The changed electrical characteristics such as threshold voltage shift, memory on- and memory off-current were maintained for more than 168 h after NBS and 24 h after PBS. It is deduced that, since the dipole alignment of the ferroelectric layer is maximized during GBS, these changes in electrical properties are caused by the remnant dipole moments still being retained during the gate sweep. The memory on- and memory off-current are controlled by GBS and the best on/off current ratio at 107 was obtained after NBS. By repeatedly alternating NBS and PBS, the electrical characteristics were reversibly changed. Our results provide the scientific and technological basis for the development of stability and performance optimization of FeOxTFTs.

Facial Soft Tissue Thickness Differences among Three Skeletal Classes in Korean Population Using CBCT.

Studies related to facial soft tissue thickness (FSTT) have been conducted since the late 19th century. Soft tissue is any tissue in the body that is not hardened by ossification or calcification processes, such as bones and teeth; and varies according to sex, age, race, and nutritional status. Forensically, soft tissue thickness plays an important role in cases where a cadaver has no unique characteristics; and the remains cannot be identified through DNA analysis, fingerprints, or examination of dental records. Therefore, the results of the current study suggest that the average thickness of the three skeletal classes (i.e., straight, concave, and convex) should be used for face restoration and forensic art research. It is thought that the current study's results will be invaluable in the fields of forensic science, forensic art, anthropology, and dentistry. As a result, gender differences were observed in all classes, and the facial tissue thickness in Korean adults differed according to gender and occlusion type.

Pulse Broadening Effects on Ranging Performance of a Laser Altimeter with Return-to-Zero Pseudorandom Noise Code Modulation.

A laser altimeter using code modulation techniques receives a backscattered pulse wider than the transmitted rectangular pulse when scanning a rough or sloped target surface. This leads to degrading the ranging performance in terms of signal-to-noise ratio (SNR) and detection probability. Unlike the pulsed techniques, little work has focused on the pulse broadening effect of the code modulation techniques. In this study, mathematical models were derived to investigate the pulse broadening effect on the ranging performance of a return-to-zero pseudorandom noise (RZPN) laser altimeter. Considering that the impulse response can be approximated by a Gaussian function, the analytical waveform was derived using a new flat-topped multi-Gaussian beam (FMGB) model. The closed-form expressions were also analytically derived for a peak cross-correlation, SNR, and detection probability in terms of the pulse broadening effect. With the use of a three-dimensional model of asteroid Itokawa for practical surface profiles, the analytical expressions were validated by comparing to the results obtained from numerical simulations. It was also demonstrated that the pulse broadening effect dropped down the peak cross-correlation and then deteriorated the ranging performance. These analytical expressions will play an important role in not only designing a laser altimeter using the RZPN code modulation technique but also analyzing its ranging performance.

Analysis of Space Debris Orbit Prediction Using Angle and Laser Ranging Data from Two Tracking Sites under Limited Observation Environment.

The global electro-optical (EO) and laser tracking sensor network was considered to investigate improvements to orbit prediction (OP) accuracy of space debris by combining angle and laser ranging data. However, it is worth noting that weather, schedule and visibility constraints can frequently limit the operations of such sensors, which may not result in sufficient tracking data for accurate OP. In this study, several 1-day OP results for low Earth orbit (LEO) space debris targets were demonstrated under a limited observation environment to verify the OP accuracy through the combination of angle and laser ranging data from two sites. For orbit determination (OD) processes, it was considered to analyze the OP accuracy by one site providing both 2-day arc angle data and 1-day arc laser ranging data, while the other was limited to 1-day arc angle data. In addition, the initial ballistic coefficient ( B C ) application method was proposed and implemented for the improvement of OD/OP accuracy, which introduces the modified correction factor depending on the drag coefficient. In the cases of combining the angle and laser ranging data, the OP results show the 3D position difference values are below 100 m root mean square (RMS) with small position uncertainty. This value satisfies the target OP accuracy for conjunction assessments and blind laser ranging (about 50-100 m at 1000 km altitude). The initial B C application method also shows better OP accuracy than the method without the correction factor.

Orbit Determination of Korean GEO Satellite Using Single SLR Sensor.

Geostationary Earth Orbit (GEO)-Korea Multi-Purpose Satellite (KOMPSAT)-2B (GK-2B) is a Korean geostationary Earth orbit (GEO) satellite that is scheduled to be launched in 2020 for meteorological and ocean monitoring. While the primary orbit determination (OD) for GK-2B is by ground-based radar observations and the expected orbit precision is less than 1 km, a satellite laser ranging (SLR) technique has been selected as a subsidiary OD method to verify/complement/enhance primary OD results. In general, the available time and equipment for observing GEO satellites with SLR are limited. Furthermore, because the optical sensors mounted on GK-2B may be defected by laser, only a domestic single SLR station would obtain the tracking data. This research presents the mitigation of these drawbacks to improve orbit precision. Observation data generation and the associated OD of GK-2B are performed by considering numerical SLR data analysis on Compass-G1, a Chinese GEO navigation satellite, and Chinese SLR station at Changchun. With the OD performed for two scenarios with the varying number of observations, the 3D position error is 24.01 m when 13 observations per day are obtained, while the error becomes 43.46 m when 9 observations per day are obtained. To verify these results, the OD of Compass-G1 using actual SLR data from Changchun station is performed to yield 31.89 m for 3D error, which is favorable compared with the external precise ephemeris by GeoForschungsZentrum (GFZ) analysis center. Therefore, the OD based on single SLR station is applicable to estimating the orbit within less than 100 m.

Synthesis of Dicyclopentadiene Oligomer Over Nanoporous Al-MCM-41 Catalysts.

One step reaction composed of DCPD oligomerization and DCPD oligomer isomerization was investigated over nanoporous Al-MCM-41 catalysts. The effects of aluminum grafting over MCM-41 on the catalyst characteristics were studied with respect to the synthesis of TCPD isomer. Physical and chemical properties of the catalysts were analyzed by N2 adsorption, temperature-programmed desorption of ammonia, and infrared spectroscopy of adsorbed pyridine. The overall number of acid sites as well as the number of Lewis acid sites increased with increasing of aluminum content over MCM-41. When utilizing MCM-41 and Al-MCM-41 as the catalyst, DCPD oligomerization reaction activity greatly increased compared to the thermal reaction. The highest TCPD isomer selectivity over the Al-MCM-41 catalyst with the highest aluminum content could be ascribed to the largest amount of acid sites. This study showed an increased level of TCPD isomer selectivity by an increasing level of Lewis acid sites through aluminum addition over MCM-41.

Synthesis of Tricyclopentadiene Over Nanoporous MCM-41 Catalysts.

The objective of this study is to evaluate the catalytic potential of metal oxide/MCM-41 catalysts in dicyclopentadiene oligomerization/dicyclopentadiene oligomer isomerization. Molybdenum oxide, tungsten oxide, and titanium oxide were loaded on MCM-41 using the modified atomic layer deposition method. The amount of the acid site with weak strength has been increased through metal oxide deposition. The oligomer yield in dicyclopentadiene oligomerization/dicyclopentadiene oligomer isomerization did not change with increasing of the amount of acid site. The highest tricyclopentadiene isomer selectivity over the MoO3/MCM-41 catalyst could be attributed to having the highest overall number of acid sites among the catalysts.