ABSTRACT
A new (3) and three known germacrane-type sesquiterpenoids were isolated from the chloroform-soluble fraction of the methanolic extract of the bark of Magnolia kobus (Magnoliaceae) through repeated silica gel and Sephadex LH-20 column chromatography. Their chemical structures were elucidated as costunolide (1), parthenolide (2), isobisparthenolidine (3), and bisparthenolidine (4) by spectroscopic analysis. Compounds 1-4 exhibited cytotoxicity against human A549, SK-OV-3, SK-MEL-2, and HCT15 tumor cells.
Subject(s)
Antineoplastic Agents, Phytogenic/chemistry , Antineoplastic Agents, Phytogenic/pharmacology , Magnolia/chemistry , Sesquiterpenes, Germacrane/chemistry , Sesquiterpenes, Germacrane/pharmacology , Sesquiterpenes/chemistry , Sesquiterpenes/pharmacology , Cell Line, Tumor , Cell Survival/drug effects , Chromatography, High Pressure Liquid , Drug Screening Assays, Antitumor , Humans , Magnetic Resonance Spectroscopy , Plant Bark/chemistry , Spectrophotometry, Infrared , Spectroscopy, Fourier Transform InfraredABSTRACT
The methanolic extract of the roots of Polygonum multiflorum (Polygonaceae) was found to show inhibitory activity towards farnesyl protein transferase (FPTase). Bioassay-guided fractionation of the methanolic extract resulted in the isolation of two anthraquinone glycosides, as inhibitors of FPTase. These compounds inhibited the FPTase activity in a dose-dependent manner.
Subject(s)
Alkyl and Aryl Transferases/antagonists & inhibitors , Anthraquinones/pharmacology , Enzyme Inhibitors/pharmacology , Glycosides/pharmacology , Polygonum , Anthraquinones/chemistry , Anthraquinones/isolation & purification , Biological Assay , Chemical Fractionation , Dose-Response Relationship, Drug , Enzyme Inhibitors/chemistry , Enzyme Inhibitors/isolation & purification , Glycosides/chemistry , Glycosides/isolation & purification , Methanol/chemistry , Molecular Structure , Plant Roots , Polygonum/chemistry , Solvents/chemistryABSTRACT
We have isolated a new prenylated chalcone from the roots of Sophora flavescens (Leguminosae). We determined that structure of this compound is 7,9,2',4'-tetrahydroxy-8-isopentenyl-5-methoxychalcone (1) on the basis of spectroscopic analysis (1D and 2D NMR data). Compound 1 exhibited potent cytotoxicity against human acute promyelocytic (HL60), mouse lymphocytic (L1210) and human histiocytic (U937) leukemia cells.
Subject(s)
Antineoplastic Agents, Phytogenic/isolation & purification , Chalcones/isolation & purification , Sophora/chemistry , Animals , Antineoplastic Agents, Phytogenic/pharmacology , Cell Line, Tumor , Chalcones/chemistry , Chalcones/pharmacology , Humans , Magnetic Resonance Spectroscopy , Mice , Protein PrenylationABSTRACT
Three known isoquinoline alkaloids were isolated from the chloroform-soluble fraction of the methanolic extract of the aerial parts of Corydalis incisa (Papaveraceae) through repeated column chromatography. Their chemical structures were elucidated as corynoline (1), corynoloxine (2) and 6-oxocorynoline (3) using spectroscopic analysis. Compounds 1-3 exhibited cytotoxicity against human A549, SK-OV-3, SK-MEL-2 and HCT15 tumor cells.
Subject(s)
Alkaloids/pharmacology , Antineoplastic Agents, Phytogenic/pharmacology , Corydalis/chemistry , Isoquinolines/pharmacology , Alkaloids/isolation & purification , Antineoplastic Agents, Phytogenic/isolation & purification , Cell Adhesion/drug effects , Cell Line, Tumor , Cell Survival/drug effects , Dose-Response Relationship, Drug , Drug Screening Assays, Antitumor , Humans , Isoquinolines/isolation & purification , Molecular Structure , Neutrophils/cytology , Plant Components, Aerial/chemistry , Structure-Activity RelationshipABSTRACT
The methanolic extract of the aerial parts of Persicaria thunbergii was found to show inhibitory activity on Farnesyl Protein Transferase (FPTase). Bioassay-guided fractionation of the methanolic extract resulted in the isolation of isorhamnetin, as an inhibitor on FPTase. This compound inhibited FPTase activity in a dose-dependent manner, and the IC50 value of isorhamnetin was 37.5 microM.
Subject(s)
Alkyl and Aryl Transferases/antagonists & inhibitors , Enzyme Inhibitors/pharmacology , Flavonols/pharmacology , Polygonaceae/chemistry , Chromatography, Thin Layer , Dose-Response Relationship, Drug , Enzyme Inhibitors/isolation & purification , Farnesyltranstransferase , Flavonols/isolation & purification , Magnetic Resonance Spectroscopy , Plant Extracts/pharmacology , Quercetin/analogs & derivativesABSTRACT
Eight compounds were isolated from the methanolic extract of the twigs of Celtis sinensis through repeated silica gel and Sephadex LH-20 column chromatography. Their chemical structures were elucidated as two triterpenoids, germanicol and epifriedelanol, two amide compounds, trans-N-caffeoyltyramine and cis-N-coumaroyltyramine, two lignan glycoside, pinoresinol glycoside and pinoresinol rutinoside, and two steroids by spectroscopic analysis.
Subject(s)
Anti-Inflammatory Agents, Non-Steroidal/chemistry , Anti-Inflammatory Agents, Non-Steroidal/isolation & purification , Antineoplastic Agents/chemistry , Antineoplastic Agents/isolation & purification , Ulmaceae , Plant Extracts/chemistry , Plant Extracts/isolation & purification , Plant StemsABSTRACT
In a bioassay-guided search for acetylcholinesterase inhibitors from Korean natural resources, four isoquinoline alkaloids, corynoxidine (1), protopine (2), palmatine (3), and berberine (4) have been isolated from the methanolic extract of the aerial parts of Corydalis speciosa. Structures of these compounds were elucidated on the basis of spectroscopic techniques. These compounds inhibited acetylcholinesterase activity in a dose-dependent manner, and the IC50 values of compounds 1-4 were 89.0, 16.1, 5.8, and 3.3 microM, respectively.
Subject(s)
Cholinesterase Inhibitors/isolation & purification , Corydalis/chemistry , Plant Extracts/isolation & purification , Acetylcholinesterase/metabolism , Animals , Benzophenanthridines , Berberine/chemistry , Berberine/pharmacology , Berberine Alkaloids/chemistry , Berberine Alkaloids/isolation & purification , Berberine Alkaloids/pharmacology , Cholinesterase Inhibitors/chemistry , Cholinesterase Inhibitors/pharmacology , Dose-Response Relationship, Drug , Kinetics , Magnetic Resonance Spectroscopy/methods , Male , Mice , Mice, Inbred ICR , Plant Extracts/chemistry , Plant Extracts/pharmacology , Plant Leaves/chemistry , Plant Stems/chemistryABSTRACT
All ten compounds were isolated from the methanolic extract of the whole plants of Diodia teres through repeated silica gel and Sephadex LH-20 column chromatography. Their chemical structures were elucidated as three iridoid glycosides, asperuloside, geniposidic acid and asperulosidic acid, a coumarin glycoside, scopolin, and six flavonoids, rutin, kaempferol-3-O-rutinoside, quercitrin, astragalin, isoquercitrin and quercetin by spectroscopic analysis.
