ABSTRACT
We investigated the pharmacokinetic profile of (R)- and (S)-zaltoprofen (ZPF) in rats using rapid and selective liquid chromatography with solid-phase extraction (SPE). The ZPF enantiomers were extracted from a small volume of plasma (0.2 mL) by means of SPE using cartridges and were analyzed on a Chiralcel OJ-H (4.6 mm × 150 mm, 5 µm) column with ultraviolet detection at 244 nm. The lower limit of quantification of the ZPF enantiomers in plasma was 0.1µg/mL. The validated method was successfully applied to chiral pharmacokinetic studies of oral administration of racemic ZPF to rats. (S)-ZPF showed significantly higher AUC, T(max), and C(max) and a longer half-life than (R)-ZPF, indicating higher bioavailability of the (S)-isomer. A total of 8 samples (about 12% of the total number of samples) were selected for incurred sample reanalysis (ISR). The % difference between the re-assay concentrations and the original concentrations were all less than 15% of their mean values and met the acceptance criteria for ISR.
Subject(s)
Benzopyrans/pharmacokinetics , Chromatography, High Pressure Liquid , Propionates/pharmacokinetics , Solid Phase Extraction , Administration, Oral , Animals , Area Under Curve , Benzopyrans/administration & dosage , Benzopyrans/blood , Benzopyrans/chemistry , Biological Availability , Calibration , Cellulose/analogs & derivatives , Cellulose/chemistry , Chromatography, High Pressure Liquid/standards , Drug Stability , Half-Life , Isomerism , Limit of Detection , Linear Models , Metabolic Clearance Rate , Propionates/administration & dosage , Propionates/blood , Propionates/chemistry , Rats , Rats, Sprague-Dawley , Reference Standards , Reproducibility of Results , Sensitivity and Specificity , Solid Phase Extraction/standards , Spectrophotometry, UltravioletABSTRACT
The method for the chiral analysis of (-)-yatein was developed and the distribution of this component in the plants of three genera like Juniperus, Thuja and Chamaecyparis belonging to Cupressaceae family was examined. The chiral analysis of (-)-yatein from the plants was carried out by high performance liquid chromatography on (R,R)-Whelk-O1 column using 81 v/v% methanol as mobile phase. The yatein content in the leaves of Juniperus was the highest in compare with that of the other two genera, providing the possibility of the chemical discrimination of the plants in Juniperus from the other plants in the Cupressaceae family. In general, the yatein content in the leaves was much higher than that in the twigs. This method could be applied for the quality control of (-)-yatein in the plants belonging to Cupressaceae family.
