ABSTRACT
Vitamin-E-loaded nanoemulsion (Vit E-NE) was produced, and the effects of repeated oral administration of Vit E-NE (2 g/kg/day) for five days on hepatic gene expression and serum metabolites were investigated in rats. The mean particle diameter and zeta potential of Vit E-NE was 112 nm and 56 mV, respectively. Vit E-NE administered rats showed significantly higher triglyceride content than of standard diet (control) or Vit E control emulsion (Vit E-CE) group but no toxicity symptoms were found in blood biochemical analysis. Next generation sequencing analysis of rat liver revealed that several genes related to energy and xenobiotic metabolism (CYP1A1 and glutathione S-transferase) were significantly altered. Serum metabolites (B-hydroxybutyrate and palmitoleic acid) indicating ketone body production and activation of stearoyl-CoAdesaturase were significantly increased by administration of Vit E-NE. The results of this study suggest that excessive consumption of edible nano-sized food ingredients can possibly cause adverse effects.
Subject(s)
Liver/drug effects , Vitamin E/adverse effects , Administration, Oral , Animals , Cytochrome P-450 CYP1A1/genetics , Cytochrome P-450 CYP1A1/metabolism , Emulsions/administration & dosage , Emulsions/adverse effects , Glutathione Transferase/genetics , Glutathione Transferase/metabolism , High-Throughput Nucleotide Sequencing , Liver/enzymology , Male , Rats , Vitamin E/administration & dosageABSTRACT
The hypocholesterolemic effects of Lactobacillus acidophilus 43121 (43121) and a mixture of Lactobacillus casei and Bifidobacterium longum (MIX) were studied in hypercholesterolemia-induced pigs. Serum total cholesterol was decreased by supplementation of either 43121 or MIX, although, high-density lipoprotein cholesterol was not changed. The hypocholesterolemic effect of 43121 and MIX was mainly due to bile acid dehydroxylation, this effect being supplementation-time dependent.
Subject(s)
Bifidobacterium/physiology , Cholesterol/blood , Feces , Hypercholesterolemia/microbiology , Lacticaseibacillus casei/physiology , Lactobacillus acidophilus/physiology , Sterols/metabolism , Animals , Hypercholesterolemia/blood , Hypercholesterolemia/metabolism , SwineABSTRACT
This study examined the effects of Lactobacillus acidophilus ATCC 43121 (LAB) on cholesterol metabolism in hypercholesterolemia-induced rats. Four treatment groups of rats (n = 9) were fed experimental diets: normal diet, normal diet+LAB (2 x 10(6) CFU/day), hypercholesterol diet (0.5% cholesterol, w/w), and hypercholesterol diet + LAB. Body weight, feed intake, and feed efficiency did not differ among the four groups. Supplementation with LAB reduced total serum cholesterol (25%) and VLDL + IDL + LDL cholesterol (42%) in hypercholesterol diet groups, although hepatic tissue cholesterol and lipid contents were not changed. In the normal diet group, cholesterol synthesis (HMG-CoA reductase expression), absorption (LDL receptor expression), and excretion via bile acids (cholesterol 7 alpha-hydroxylase expression) were increased by supplementation with LAB, and increased cholesterol absorption and decreased excretion were found in the hypercholesterol diet group. Total fecal acid sterols excretion was increased by supplementation with LAB. With proportional changes in both normal and hypercholesterol diet groups, primary bile acids (cholic and chenodeoxycholic acids) were reduced, and secondary bile acids (deoxycholic and lithocholic acids) were increased. Fecal neutral sterol excretion was not changed by LAB. In this experiment, the increase in insoluble bile acid (lithocholic acid) reduced blood cholesterol level in rats fed hypercholesterol diets supplemented with LAB. Thus, in the rat, L. acidophilus ATCC 43121 is more likely to affect deconjugation and dehydroxylation during cholesterol metabolism than the assimilation of cholesterol into cell membranes.
