ABSTRACT
Foam cell formation is the most important process in atherosclerosis, and low density lipoprotein oxidation by reactive oxygen species (ROS) is the key step in the conversion of macrophages to foam cells. This study reveals the control mechanism of the gene for NADPH oxidase 1 (Nox1), which produces ROS in the formation of foam cells by stimulating TLR4. Treatment of macrophages by the TLR4 agonist LPS stimulated ROS production and ROS-mediated macrophage to foam cell conversion. This LPS-induced ROS production and foam cell formation could be abrogated by pretreatment of macrophages with N-acetyl cysteine or apocynin. LPS increased Nox1 promoter activity, and resultant expression of mRNA and protein. Small interfering RNA mediated inhibition of Nox1 expression decreased LPS-induced ROS production and foam cell formation. LPS-mediated Nox1 expression and the responses occurred in a calcium-independent phospholipase A(2) (iPLA(2))-dependent manner. The iPLA(2)beta-specific inhibitor S-BEL or iPLA(2)beta small interfering RNA attenuated LPS-induced Nox1 expression, ROS production, and foam cell formation. In addition, activation of iPLA(2)beta by LPS caused Akt phosphorylation and was followed by increased Nox1 expression. These results suggest that the binding of LPS and TLR4 increases Nox1 expression through the iPLA(2)beta-Akt signaling pathway, and control ROS production and foam cell formation.
Subject(s)
Foam Cells/immunology , Gene Expression Regulation/immunology , Group IV Phospholipases A2/immunology , NADH, NADPH Oxidoreductases/immunology , Proto-Oncogene Proteins c-akt/immunology , Signal Transduction/immunology , Animals , Atherosclerosis/immunology , Atherosclerosis/metabolism , Blotting, Western , Calcium Signaling/immunology , Cell Line , Flow Cytometry , Foam Cells/metabolism , Gene Expression , Group IV Phospholipases A2/metabolism , Lipopolysaccharides/immunology , Mice , Microscopy, Confocal , NADH, NADPH Oxidoreductases/metabolism , NADPH Oxidase 1 , Proto-Oncogene Proteins c-akt/metabolism , RNA, Small Interfering , Reactive Oxygen Species/immunology , Reactive Oxygen Species/metabolism , Reverse Transcriptase Polymerase Chain Reaction , Toll-Like Receptor 4/immunology , Toll-Like Receptor 4/metabolismABSTRACT
In this study, we show that activation of toll-like receptor (TLR)4 by lipopolysaccharide (LPS) induces cyclooxygenase-2 (COX-2) expression, which results in prostaglandin (PG)I2 formation in macrophages. The LPS-stimulated COX-2 expression and PGI2 release were accompanied by production of the potent angiogenic cytokine, vascular endothelial growth factor (VEGF), and these effects were suppressed by NS-398, which is a COX-2 inhibitor. Direct addition of iloprost (an analogue of PGI2) for IP receptor also induced the production of VEGF, whereas DP, FP, and TP receptor agonists did not. Inhibition of IP protein expression by micro interfering RNA blocked LPS-induced VEGF production. Additionally, macrophages transiently caused Akt phosphorylation after stimulation with LPS, and inhibition of Akt phosphorylation blocked the production of VEGF and COX-2 expression in response to LPS. Overall, this study demonstrated that engagement of TLR4 with LPS induces production of PGI2 via Akt and generates VEGF through IP receptor.
