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Methods Mol Biol ; 1224: 37-45, 2015.
Article in English | MEDLINE | ID: mdl-25416247

ABSTRACT

Sesame (Sesamum indicum L.) is an important oilseed crop grown in India, China, Korea, Russia, Turkey, Mexico, South America, and several countries of Africa. Sesame seeds are rich in oil, proteins, unsaturated fatty acids, vitamins, minerals, and folic acid. Nearly 70% of the world's sesame is processed into oil and meal, while the remainder is channeled to food and confectionery industries. Production of sesame is limited by several fungal diseases, water logging, salinity, and shattering of seed capsules during harvest. Introgression of useful genes from wild species into cultigens by conventional breeding has not been successful due to postfertilization barriers. The only alternative for the improvement of S. indicum is to transfer genes from other sources through genetic transformation techniques. Here, we describe a simple, fast, and reproducible method for the Agrobacterium-mediated genetic transformation of S. indicum which may be employed for the transfer of desirable traits into this economically important oilseed crop.


Subject(s)
Agrobacterium tumefaciens/genetics , Genetic Engineering/methods , Sesamum/growth & development , Sesamum/genetics , Acclimatization , Agrobacterium tumefaciens/growth & development , Coculture Techniques , Plant Roots/genetics , Plant Roots/growth & development , Plants, Genetically Modified , Sesamum/physiology , Time Factors , Transformation, Genetic
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