ABSTRACT
BACKGROUND: Glycation is known to play a key role in complications of many pathophysiological processes. The present study was carried out to assess whether there are abnormalities of non-enzymatic glycation of proteins and hemoglobin in acute Myocardial Infarction (MI) patients. METHODS: Eighteen acute Myocardial Infarction (MI) patients and 20 healthy controls were enrolled for the present study. Fasting plasma glucose, fructosamine, glycated hemoglobin were evaluated. RESULTS: A significant rise in the mean values of fructosamine and glycated hemoglobin was found in acute myocardial patients when compared with controls. When Pearson's correlation analysis was performed, no significant correlation was found between fasting plasma glucose with either fructosamine or glycated hemoglobin levels. CONCLUSION: This data suggests an increased glycation of both plasma proteins and glycated hemoglobin in acute myocardial patients, which might be independent of prevailing glucose concentration.
ABSTRACT
BACKGROUND: Collective evidences reveal that malondialdehyde (MDA), reduced glutathione (GSH) and ascorbic acid can modulate protein glycation. We investigated the concentrations of MDA, GSH, ascorbic acid and protein glycation in asthma patients to delineate the possible association among these parameters. METHODS: Blood was collected from 18 asthma patients and 16 age and sex matched control subjects. Glycated hemoglobin (HbA1C), GSH, MDA, vitamin C, fructosamine and glucose were assessed in both groups. The effect of H2O2 on glycation of hemoglobin was studied by incubating normal healthy erythrocytes with either 5 or 50 mmol/l glucose concentration. RESULTS: Plasma of asthma patients revealed significantly higher concentrations of lipid peroxides and fructosamine concentrations than the matched controls. Glycated hemoglobin concentrations were also found to be significantly increased. Ascorbic acid and GSH concentrations were decreased significantly in the test group when compared with the healthy control group. When the effects of fasting glucose, GSH and ascorbic acid on the concentrations of HbA1C and fructosamine were refuted by partial correlation analysis, MDA was found to be a significant determinant of HbA1c and fructosamine in patients with asthma. The in vitro model with human erythrocytes showed an enhancement of protein glycation by H2O2. CONCLUSION: An increased glycation of proteins was found in asthma patients. These data also support the premise that lipid peroxides per se do have a role to play in glycation of hemoglobin and plasma proteins.
