ABSTRACT
The shells of freshwater snails are discarded as waste, which qualify as biological materials with prospective multiple uses. To substantiate this proposition, an attempt was made to elucidate the physical and chemical properties of the shells of three freshwater snails, namely, Bellamya bengalensis, Pila globosa, and Brotia costula. The shells were prepared for electron microscopy and assessment of the calcium carbonate content, apart from the Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), energy-dispersive X-ray spectroscopy (EDS), and nanoindentation studies. The results indicated that the calcium carbonate content (y) of the shells ranged between 87 and 96% of the total weight (x) and complied with a power regression equation: y = 0.801x 1.016; R 2 = 0.994; r = +0.998; P < 0.001. Observations through SEM depicted different snail species-specific arrangement patterns of calcium carbonate crystals in the diverse layers of shells. The XRD, FTIR, and EDS observations revealed the dominance of the aragonite form of the calcium carbonate crystal in the microstructures of each snail shell with the occurrence of different shell surface functional groups. The Brunauer-Emmett-Teller analysis elucidated the surface textures of shell dust taken from each snail species; in addition, the nanohardness properties indicate the shells as a tough biocomposite exoskeleton. Species-specific variations in the shell morphology, microstructure, and calcium carbonate content were prominent for the three freshwater snails considered for the study. Nonetheless, the physical and chemical properties substantiate that the shells of B. bengalensis, P. globosa, and B. costula qualify as biological materials for sustainable use in various fields including bioremediation, biocatalyst, biomedical applications, and a source of lime. Since the shells of the freshwater snails are discarded as aquaculture waste, subsequent use as a biological material will support the "waste made useful" paradigm in sustainability, both from ecological and economic perspectives.
ABSTRACT
The shell of the freshwater mussel (Mollusca: Bivalvia) is a composite biological material linked with multifunctional roles in sustaining ecosystem services. Apart from providing mechanical strength and support, the shell is an important site for adherence and growth of multiple types of algae and periphyton. Variations in the shell architecture are observed in the mussels both within a species and among different species. Considering the prospective utility of the shell of the freshwater mussels as a biological material, an assessment of the shell characteristics was accomplished using Corbicula bensoni and Lamellidens marginalis as model species. The calcium carbonate (CaCO3) content of the shells, physical features and mechanical strength were assessed along with the morphometric analysis. The CaCO3 content of the shell (upto 95% to 96% of the shell weight) of both the mussels was positively correlated with the shell length, suggesting increased deposition of CaCO3 in shells with the growth of the species. The cross sectioned views of FE-SEM images of the shells exhibited distinct layered structure with external periostracum and inner nacreous layer varying distinctly. In the growing region, the growth line was prominent in the mussel shells revealed through the FESEM images. In addition XRD, FTIR and EDS studies on the mussel shells confirmed the existence of both aragonite and calcite forms of the calcium carbonate crystals with the incidence of various functional groups. The mechanical strength of the mussel shells was explored through nanoindentation experiments, revealed significant strength at the nanoparticle level of the shells. It was apparent from the results that the shell of the freshwater mussel L. marginalis and C. bensoni qualify as a biological material with prospective multiple applications for human well-being and sustaining environmental quality.
