ABSTRACT
AIM: The aim of this study was to investigate the relationship between inter-individual global DNA methylation and diabetes predisposing factors. METHODS: The 5-methyl cytosine content was assessed by reverse phase high pressure liquid chromatography (RP-HPLC) of peripheral blood leukocytes obtained from 178 type 2 diabetes patients to determine individual global DNA methylation status. RESULTS: There was a positive significant correlation between diabetes duration and DNA methylation levels (P=0.002) with increasing levels of DNA methylation associated with age (P=0.047). There was no significant correlation between DNA methylation levels and HbA1c (P=0.15). No significant differences were observed between patients with and without diabetes predisposing factors including: hypertension (P=0.772), dyslipidemia (P=0.617), insulin resistance (homeostatic model assessment index) (P=0.156) and obesity (P=0.609). As such, the duration of diabetes (>10 years) was the most important predictor of global DNA methylation levels in diabetic patients after adjusting for age and sex (P=0.023). CONCLUSION: Our findings indicate that chronic hyperglycemic exposure plays an independent role in global DNA methylation levels in type 2 diabetes patients.
Subject(s)
Chromatography, High Pressure Liquid , DNA Methylation , Diabetes Mellitus, Type 2/blood , Diabetes Mellitus, Type 2/diagnosis , Albuminuria/urine , Biomarkers/blood , Body Mass Index , Cross-Sectional Studies , Diabetes Mellitus, Type 2/complications , Diabetes Mellitus, Type 2/urine , Disease Progression , Dyslipidemias/complications , Female , Glycated Hemoglobin/metabolism , Humans , Hypertension/complications , Leukocytes/metabolism , Male , Middle Aged , Obesity/complications , Predictive Value of Tests , Reproducibility of Results , Risk Factors , Sensitivity and Specificity , Severity of Illness IndexSubject(s)
Chromosomes, Human, Pair 18 , Olfaction Disorders/genetics , Chromosome Mapping , Female , Genetic Linkage , Haplotypes , Humans , Male , Olfaction Disorders/congenital , PedigreeABSTRACT
Oxidative stress status and acetylcholinesterase (AChE) activity were studied in blood samples obtained from 45 organophosphorous (OP)-formulating pesticide workers with a minimum work history of 1 year in the age range of 23-55. Controls were age-matched workers of a food-making factory. They were evaluated for oxidative stress markers, including thiobarbituric acid-reactive substances (TBARS) indicator of lipid peroxidation (LPO), ferric-reducing ability of plasma (FRAP) indicator of total anti-oxidant capacity, total thiol (SH) groups and gamma glutamyl transpeptidase (GGT) levels in blood and AChE activity in erythrocytes. The results show marked inhibition of AChE activity, increased TBARS, decreased FRAP and decreased thiol group levels in workers. The reduction in activity of AChE correlated well with increased TBARS and decreased FRAP in OP formulators. It is concluded that OP-formulating workers are exposed to more oxidative stress. The measurement of erythrocyte AChE activity in pesticide workers who formulate OPs can be a good monitoring factor and is recommended to be performed in a regular manner.
