Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 3 de 3
Filter
Add more filters










Database
Language
Publication year range
2.
Am J Physiol Regul Integr Comp Physiol ; 284(2): R564-73, 2003 Feb.
Article in English | MEDLINE | ID: mdl-12388439

ABSTRACT

The trefoil factor family peptides TFF1, TFF2, and TFF3 are important for gut mucosal protection and restitution. Keratinocyte growth factor (KGF) stimulates proliferation and differentiation of epithelial cells with potent effects on goblet cells. To investigate interactions between food intake and KGF, rats were fed ad libitum (control), fasted for 72 h, or fasted for 72 h and then refed for 72 h with or without KGF (3 mg. kg(-1). day(-1)). With fasting, goblet cell number in duodenum increased, TFF3 mRNA in duodenum and jejunum decreased, and TFF3 protein did not change or increased. KGF during fasting stimulated colonic growth, normalized TFF3 mRNA in duodenum and jejunum, and broadly upregulated gut goblet cell number and TFF3 protein expression. With fasting-refeeding, KGF increased small bowel and colonic mucosal growth, goblet cell number, and TFF3 protein but had variable effects on TFF3 mRNA. KGF induced TFF2 mRNA and protein in duodenum and jejunum with both nutritional regimens. We conclude that nutrient availability modifies rat intestinal goblet cell number, TFF3 mRNA, and the gut-trophic effects of KGF in a region-specific manner. KGF enhances TFF2 expression in proximal small bowel and increases goblet cell number and TFF3 protein content throughout the intestine independent of food intake.


Subject(s)
Fasting/physiology , Fibroblast Growth Factors/pharmacology , Gene Expression Regulation/drug effects , Goblet Cells/cytology , Goblet Cells/drug effects , Growth Substances/metabolism , Intestinal Mucosa/drug effects , Mucins , Muscle Proteins , Neuropeptides , Peptides/metabolism , Animals , Blotting, Western , Body Weight , Cell Count , Eating , Fibroblast Growth Factor 7 , Food Deprivation , Goblet Cells/metabolism , Growth Substances/genetics , Immunohistochemistry , Intestinal Mucosa/cytology , Intestinal Mucosa/growth & development , Intestinal Mucosa/metabolism , Male , Peptides/genetics , RNA, Messenger/genetics , RNA, Messenger/metabolism , Rats , Rats, Sprague-Dawley , Trefoil Factor-2 , Trefoil Factor-3
3.
Am J Clin Nutr ; 75(5): 922-30, 2002 May.
Article in English | MEDLINE | ID: mdl-11976168

ABSTRACT

BACKGROUND: Intestinal adaptation after massive bowel resection in animal models is characterized by increased gut-mucosal growth and expression of nutrient transporters. Few data about these indexes exist in humans with short-bowel syndrome (SBS). OBJECTIVE: The objective was to compare small-bowel and colonic mucosal growth and expression of the peptide transporter PepT1 in adults with or without SBS. DESIGN: Mucosal biopsy specimens were obtained from the small bowel and colon of 33 control subjects with intact intestine and from 13 SBS patients dependent on parenteral nutrition because of chronic malabsorption. Gut-mucosal crypt depth, villus height, and villus width were measured, and expression of PepT1 was determined by Northern blotting, in situ hybridization, and immunohistochemistry. RESULTS: The indexes of small-bowel and colonic mucosal growth were not significantly different between the 2 groups. PepT1 expression was high in the apical region of duodenal, jejunal, and ileal villus epithelial cells; low in absorptive colonocytes; and not significantly different in the distal small intestine of the 2 groups. However, the abundance of PepT1 mRNA in the colon of SBS patients was more than 5-fold that in control subjects (P < 0.01). CONCLUSIONS: Gut adaptation in SBS patients does not appear to involve an increase in gut-mucosal crypt depth or villus size. PepT1 is abundant along the small-bowel brush border in humans; expression in the colon indicates that the large intestine has a mechanism for luminal di- and tripeptide transport. Up-regulation of colonic PepT1 in SBS may adaptively improve accrual of malabsorbed di- and tripeptides, independent of changes in the mucosal surface area.


Subject(s)
Carrier Proteins/metabolism , Colon/metabolism , Intestinal Mucosa/metabolism , Short Bowel Syndrome/metabolism , Symporters , Adult , Carrier Proteins/genetics , Colon/pathology , Female , Humans , Intestinal Absorption , Intestinal Mucosa/pathology , Intestine, Small/pathology , Male , Middle Aged , Peptide Transporter 1 , RNA, Messenger/metabolism , Reference Values , Short Bowel Syndrome/pathology , Tissue Distribution , Up-Regulation
SELECTION OF CITATIONS
SEARCH DETAIL
...