ABSTRACT
The influence of UV-light (240-390 nm) in doses 151-3020 J/m2 on the nature of the death of lymphocytes cells of donor's blood (using markers of apoptotic and necrotic death of cells) and on the level of expression of the marker of apoptotic pre-preparation--CD95-receptor has been investigated. We have shown that UV-radiation increases expression of CD95-receptors which is caused mainly by de novo synthesis of the receptors. It has been revealed that during daily incubation of photo-modified lymphocytes (151 and 755 J/m2) without autological blood cell death occurs by receptor-involved apoptosis. Exposure to high doses of radiation (1510 and 3020 J/m2) causes massive necrotic death of immunocytes. The use of autologous blood plasma during incubation of photo-modified lymphocytes allows decreasing the number of both apoptotic and necrotic cells.
Subject(s)
Apoptosis/radiation effects , Lymphocytes/radiation effects , fas Receptor/biosynthesis , Apoptosis/drug effects , Apoptosis/genetics , Biomarkers/metabolism , Cells, Cultured , Culture Media/chemistry , Culture Media/pharmacology , Dose-Response Relationship, Radiation , Gene Expression , Humans , Lymphocytes/drug effects , Lymphocytes/metabolism , Lymphocytes/pathology , Necrosis/genetics , Necrosis/metabolism , Necrosis/pathology , Plasma , Ultraviolet Rays , fas Receptor/agonists , fas Receptor/geneticsABSTRACT
Structural and functional state of human blood lymphocytes after exposure to UV light (240-390 nm) in doses of 151-1359 J/m(2) was studied by methods of laser flow cytofluorometry, indirect immunofluorescence, and fluorescent probes. Using a combination of these methods, we have showed that UV light in the specified doses induced changes in the surface phenotype of T cells: stimulation or suppression of the expression of antigen-recognizing receptor complex molecules (CD3, CD4, and CD8 markers) and their redistribution on the surface of immunocompetent cells (capping effect) with the formation of receptor clusters of various types.
Subject(s)
B-Lymphocytes/radiation effects , CD3 Complex/genetics , CD4 Antigens/genetics , CD8 Antigens/genetics , Cell Membrane/radiation effects , T-Lymphocytes/radiation effects , Anilino Naphthalenesulfonates , B-Lymphocytes/immunology , B-Lymphocytes/metabolism , CD3 Complex/immunology , CD4 Antigens/immunology , CD8 Antigens/immunology , Cell Membrane/metabolism , Cells, Cultured , Flow Cytometry , Fluorescent Antibody Technique, Indirect , Fluorescent Dyes , Gene Expression/radiation effects , Humans , Spectrometry, Fluorescence , T-Lymphocytes/immunology , T-Lymphocytes/metabolism , Ultraviolet RaysABSTRACT
The influence of a wide range of doses of UV-light (240-390 nm) on the expression level of receptor complex molecules (CD3, CD4, CD8 markers) on the membrane surface of human blood T-lymphocytes has been studied using flow cytometry and enzyme linked immunosorbent assay. UV-light at small and medium doses (15 1, 453 and 906 J/m2) has been established to have a unidirectional (activating) effect on the expression level of receptor complex molecules, and at a high dose of 1359 J/m2 it can either increase (CD4 and CD8 markers) or reduce (CD3 complexes) the quantity of the analyzed molecules on the membrane surface of T-lymphocytes.
Subject(s)
CD3 Complex/biosynthesis , CD4 Antigens/biosynthesis , CD8 Antigens/biosynthesis , Receptors, Antigen, T-Cell/biosynthesis , T-Lymphocytes/radiation effects , Ultraviolet Rays , Cell Membrane/immunology , Cell Membrane/metabolism , Cell Membrane/radiation effects , Cells, Cultured , Dose-Response Relationship, Radiation , Flow Cytometry , Humans , Immunoenzyme Techniques , T-Lymphocytes/immunology , T-Lymphocytes/metabolismABSTRACT
Serum activity of tartrate-resistant acid phosphatase 5b (TRAP 5b) in patients with breast cancer and prostate cancer having bone metastases was much higher than in healthy donors and patients without skeletal injuries. TRAP 5b activity in patients with breast cancer and multiple bone metastases surpassed that in patients with single bone metastases. The mean activity of TRAP 5b and range of enzyme activity in women treated with bisphosphonates were significantly lower than in patients not receiving antiresorptive therapy. Diagnostic sensitivity and specificity of TRAP 5b as a marker of skeletal metastases in patients with breast cancer were 82 and 87%, respectively. In patients with prostate cancer these indexes were 71 and 83.4%, respectively. Detection of this marker in tumor patients holds much promise for early diagnostics of bone metastases, estimation of the severity of skeletal metastases, and monitoring of the efficiency of bisphosphonate therapy.
