ABSTRACT
We studied mobilization of bone marrow stem cells the under conditions of boost-therapy with granulocytic CSF and erythropoietin. Immunophenotyping of the peripheral blood was carried out in patients after transplantation of tissue-engineered trachea during the perioperative period. We observed an increase in hemopoietic stem cell pool and appearance of multipotent mesenchymal stromal cells in systemic circulation, their maximum concentration being recorded on days 10-14 after surgery. These data indicated a positive effect of growth factors on mobilization of stem cells.
Subject(s)
Granulocyte Colony-Stimulating Factor/therapeutic use , Mesenchymal Stem Cells/physiology , Tracheal Stenosis/surgery , Erythropoietin/therapeutic use , Humans , Mesenchymal Stem Cells/drug effects , Tissue Scaffolds , Trachea/surgery , Tracheal Stenosis/blood , Tracheal Stenosis/drug therapyABSTRACT
The authors admit the risks of blood transfusion, as well as the fact that the blood is a limited resource. These conclusions became the basis of the research in order to make an analysis and develop transfusion strategies in the hospital. An assessment of blood components application was performed in specific cases. There was changed the management of blood transfusion and further monitoring was continued. It was shown that the efficacy of an introduction of a new transfusion strategy confirmed the decrease of the rate of inappropriate blood transfusions, the quantity of patients who obtained transfusion of allogenic blood components and as a result, the new methods reduced the number of blood transfusions.
