[Placental morphology in inherited thrombophilia].

OBJECTIVE: To study placental morphological changes in inherited thrombophilia. SUBJECT AND METHODS: A morphometric method was used to examine placentas from 37 patients divided into 3 groups: 1) 13 pregnant women with verified inherited thrombophilia; 2) 14 pregnant women with inherited thrombophilia and signs of placental inflammatory manifestations (basal deciduitis, intervillositis, placentitis); 3) 10 women with normal pregnancy (a control group). RESULTS: In inherited thrombophilia, the placentas exhibited morphological manifestations of chronic placental insufficiency as pathological immaturity with a predominance of intermediate differentiated villi and as dissociated maturity of cotyledones, with a substantial reduction in the specialized terminal villi, excessive intervillous fibrinoid formation and villous immuring, evolving pseudoinfarctions, fewer capillaries in the terminal villi, and their increased stromal proportion. At the same time, no significant differences were found in the morphological pattern in inherited thrombophilia concurrent with placental inflammatory changes. CONCLUSION: In inherited thrombophilia, there is chronic placental insufficiency caused by delayed development of the shaggy chorion.

Mixed lymphocyte reaction blocking factors (MLR-Bf) as potential biomarker for indication and efficacy of paternal lymphocyte immunization in recurrent spontaneous abortion.

OBJECTIVES: The majority of cases of unexplained recurrent spontaneous abortion (RSA) remains unclear and is found to be associated with alloimmune antibodies termed as mixed lymphocyte reaction blocking factor (MLR-Bf). The decreased production of MLR-Bf may play major role in the immunologic failure of pregnancy and can lead to abortion. The present study was aimed at evaluating MLR-Bf as potential biomarker of indication and the efficacy of immunotherapy with paternal lymphocytes (LIT) in women with RSA. MATERIALS AND METHODS: A total of 97 women with history of unexplained RSA were recruited for this prospective study. These women showed negative for MLR-Bf and registered for lymphocyte immunotherapy with husband cells. Women with autoimmune pathology or anti-phospholipid syndrome were excluded. All individuals gave their consent to participate in the study. RESULTS: We have analyzed MLR proliferative response and MLR-Bf in nonpregnant women with history of RSA before and after LIT. Following LIT, the initially low MLR proliferative response was restored at 76.6 % of women, and MLR-Bf activity in blood serum could be detected in 74 % of women. The rate of successful pregnancy was shown to be significantly higher in women positive for MLR-Bf (50/72) as compared with the MLR-Bf negative women (7/25; χ (2) = 0.0003). CONCLUSION: The data obtained demonstrate that LIT with the paternal lymphocytes in MLR-Bf negative women is accompanied by increased proliferative cell response to the paternal alloantigens and by enhanced production of soluble suppressor activity factors (MLR-Bf) that is associated with improved pregnancy outcome in women with history of RSA.

[Structural-functional transformations of the ileal lymph nodes in pregnancy complicated by blood loss]. / Strukturno-funktsional'nye preobrazovaniia podvzdoshnykh limfaticheskikh uzlov pri beremennosti, oslozhnennoi krovopoterei.

The microanatomic organization of iliac lymph nodes was studied in 24 pregnant Wistar rats (pregnancy term 21 days) after simulation of acute hemorrhage in them. A considerable decrease of a relative area occupied by a marginal sinus was revealed. This shows transport functional changes of lymph nodes in pregnancy complicated by hemorrhage.

[The morphofunctional characteristics of the thymus in rat fetuses and progeny in venous occlusion]. / Morfofunktsional'naia kharakteristika timusa u plodov i potomstva krys pri flebookkliuzii.

Using histological and morphometrical methods, morphofunctional state of the thymus was studied in fetuses and offspring of rats in physiological pregnancy and under conditions of phlebo-occlusion. Phlebo-occlusion resulted in hypertrophy of the fetus, thymus and offspring, changes in structural zones and cellular reactions.