ABSTRACT
UNLABELLED: The aim of this paper is to describe the prospective 10-year follow-up study of prognostic value of p53 protein accumulation in cancer cells in relation to the localisation of tumour in colorectum. METHODS: The p53 protein accumulation has been assessed immunohistochemically in tumour tissue samples obtained from 80 selected sporadic colorectal cancer patients. RESULTS: p53 protein accumulation was detected more often in rectum than in colon tumours. Lower survival rates were observed in patients with p53 protein accumulation in cancer cells independent of primary tumour site. Significantly lower cumulative survival rate was detected in the group of patients with left-sided colon cancer. The p53 protein accumulation was an independent prognostic factor in the left-sided colon tumours. CONCLUSIONS: The right-sided colon tumours may be developed in p53-independent manner and p53 protein accumulation in cancer cells has prognostic value only in the left-sided colorectal tumours.
Subject(s)
Adenocarcinoma, Mucinous/genetics , Colorectal Neoplasms/genetics , Tumor Suppressor Protein p53/pharmacokinetics , Adenocarcinoma, Mucinous/pathology , Adult , Aged , Colorectal Neoplasms/pathology , Female , Follow-Up Studies , Functional Laterality , Humans , Immunohistochemistry , Male , Middle Aged , Predictive Value of Tests , Prognosis , Prospective Studies , Survival Analysis , Tumor Suppressor Protein p53/analysisABSTRACT
To assess the clinico-prognostic relevance of the cell surface carbohydrate glycoprotein in normal and pathological conditions of human endometrium, Sialosyl-Tn (STn) antigen was immunohistochemically studied in normal (n = 10), hyperplastic (n = 18), and neoplastic (n = 60) endometrial lesions. There was no STn antigen reactivity in the proliferative endometrial slides, while weak staining was observed in all secretory endometria. STn expression was noted in 8/18 (44%) hyperplastic endometrial cases and in 40/60 (67%) endometrial carcinomas. Positive staining was observed throughout the cytoplasm of the glandular cancer cells, at the cell membranes, and in an intraluminar mucus. This antigen was mostly expressed heterogeneously as far as the distribution of positive cells is concerned. There was a statistically significant association between STn expression and the histological grading of cancer (p = 0.019). Advanced clinical stage (III-IV; p = 0.014) and infiltration of the myometrial wall (more than 1/2 of the myometrial wall; p = 0.004), but no STn immunoreactivity, were reported to be independent prognostic variables during follow-up. Our study shows that a) STn is not constantly expressed during the menstrual cycle, and is increased at the secretory phase of the cycle; b) Sialosyl-Tn reactivity decreases with the degree of tumor differentiation, but there was no relationship with other clinicopathological variables of cancer; c) this cell surface carbohydrate glycoprotein does not appear to predict the outcome of endometrial cancer patients.
