ABSTRACT
BACKGROUND: Published studies assessing the association of human papillomavirus (HPV) and oral cancer, often include cases with additional confounders such as alcohol, tobacco, thus, precluding the assessment of HPV as a independent risk factor. Further apart from eliciting the mere presence of HPV, it is vital that the nature of the association is delineated for eliciting a causal inference. Thus, the present study assessed the presence and nature of the association of HPV in oral cancer cases with and without known risk factors. METHODS: The study compares the prevalence of HPV in oral cancer cases with known risk factors (group 1, n=15) to oral cancer (group 2, n=15) and oral epithelial dysplasia (group 3, n=15) cases with no known risk factors using PCR. Cases which are positive for HPV were subjected to p16 and p53 immunostaining to determine potential causal inference. RESULTS: HPV 16 was detected in only 1 case of group I, 3 cases of group II, and was absent in group III. HPV 18 was negative in all the 3 groups. All the HPV positive cases were negative for p16 and positive for p53 immunostaining. CONCLUSIONS: Only a minor proportion of oral cancer cases without risk factors were positive for HPV 16. Even among these HPV 16 positive cases, the immunostaining profile (p53 positive and p16 negative) excludes the involvement of E6 and E7 mediated carcinogenesis. Thus even the weak HPV association noted in the present study cases may not be of causal nature.
ABSTRACT
INTRODUCTION: To assess the knowledge and extent of WhatsApp usage for dentistry related purposes among general dental practitioners (GDPs). MATERIALS AND METHODS: A cross-sectional study was conducted among 105 randomly selected GDPs from Pune, Maharashtra, India. Data was collected in a personalized manner by means of validated questionnaire. Results and observations: A total of 105 dentists participated in the survey: 96.19% of dentists had WhatsApp installed in their phones; 67.32% of dentists sought second opinion on WhatsApp; 60.29% of dentists received prompt replies, while 38.23% received late replies; 98.52% of dentists sent clinical photographs and radiographs for second opinion. 88.11% of dentists were a part of various 'dentistry related groups' and 72.27% of dentists told that patients ask their queries on WhatsApp. 36.76% of GDPs obtained verbal consent from the patients for sending clinical materials for second opinion. Majority of population of GDPs 63.23% (43) did not obtain any form of consent from the patients. CONCLUSION: Majority of GDPs uses WhatsApp for 'dentistry related purposes' and it has become an integral part of their day-to-day practice. GDPs should obtain written consent before sending clinical materials for second opinion. CLINICAL SIGNIFICANCE: Till date, the extent of WhatsApp usage by general dental practitioners was not reported in the literature. It appears that, WhatsApp application has become an integral part of general dental practice in India. By virtue of this, obtaining second opinion, taking appointments and solving queries of patients are no longer a time consuming events. In future, instant messaging services might play major role in providing efficient services in health care industry.
